| Project/Area Number |
07680824
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Saga Medical School |
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Principal Investigator |
MASUKO Sadahiko Saga Medical School, Department of Anatomy, Professor, 医学部, 教授 (20015100)
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| Co-Investigator(Kenkyū-buntansha) |
FUJIYAMA Fumino Saga Medical School, Department of Anatomy, Research Associate, 医学部, 助手 (20244022)
KAWANO Hitoshi Saga Medical School, Department of Anatomy, Lecturer, 医学部, 講師 (10152985)
MURATA Yuzo Saga Medical School, Department of Anatomy, Research Associate, 医学部, 助手 (20128143)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Sympathetic post gangionic neuron / Spinal cord / gangliospinal projection / Intermediolateral nucleus / Retrograde neuronal tracing / Electron microscopy / Synapse / Immunohistochemistry |
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| Research Abstract |
1) In order to determine non-descending nerve terminals in the intermediolateral nucleus (IML), rat spinal cord was transected at T6 level. Seven days after the operation, serotonin (5-HT)-immunoreactive terminals disappeared completely from the IML of lower spinal cord. However, substance P-, enkephalin-, and tyrosine hydroxylase (TH)-immunoreactive nerve terminals remained in the IML.Electron microscopic observations revealed presence of synaptic contacts between these immunoreactive terminals and IML neurons. This result indicates presence of neuronal inputs from neurons in intrinsic or sub-spinal structures to the sympathetic preganglionic neurons.
2) To examine a possible origin of TH-immunoreactive non-descending terminals in the IML,retrograde neuronal tracing experiments were made. After injection of cholera toxin B (CTB) into the spinal cord of levels T2-13, postganglionic cells located in thoracic sympathetic ganglia (T2-11) were labeled preferentially with CTB.Injections into more rostral (C6) or caudal (L1, L5) spinal cord segments resulted in few labeled ganglion cells. This result suggest that postganglionic neurons in the thoracic sympathetic ganglion project to thoracic spinal cord.
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