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A chronologic study of apoptotic cells in the cerebral hypoplasia induced by intra-uterine infection

Research Project

Project/Area Number 07680829
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionJikei University School of Medicine

Principal Investigator

TANAKA Jun-ichi  Jikei Univ.Sch.of Med., Neuropathol., Professor, 医学部, 教授 (60079712)

Co-Investigator(Kenkyū-buntansha) MAKIOKA Asao  Jikei Univ.Sch.of Med., Tropical Med., Lecturer, 医学部, 講師 (90119850)
WATABE Kazuhiko  Tokyo Metropolitan Inst.for Neurosci., Researcher, 研究員 (30240477)
MINAMITANI Motoyuki  Jikei Univ.Sch.of Med., Pediatrics, Assistant, 医学部, 助手 (00229775)
FUKUDA Takahiro  Jikei Univ.Sch.of Med., Neuropathol., Lecturer, 医学部, 講師 (60228913)
MATSUSHIMA Hiroshi  Jikei Univ.Sch.of Med., Pediatrics, Lecturer, 医学部, 講師 (70190460)
Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Keywordstoxoplasma / intra-uterine infection / mouse / fetal brain / cerebral cortical hypoplasia / apoptosis / TUNEL method / 胎生期感染 / 皮質低形成 / DNA断片
Research Abstract

One of the developing disorders in the fetal brain with toxoplasma infection is cortical hypoplasia to which apoptosis may contribute in its process. Congenital toxoplasmosis was induced by intra-peritoneal injection of Toxoplasma gondii (ME49 strain) to the pregnant mice (C57BL/6CrSlc) on gestational day 5 (E5). On E10, E12, E14, E16 and E18 apoptotic cells were demonstrated in the cerebral layrs in both infected and control groups using an in situ end-labeling (TUNEL) method. The cerebral cortex in the infected mice was thinner than in the controls and disclosed an immature laminar architecture. Apoptotic cells distributed mainly in the subependymal layr and occasionally in the intermediate zone. Numbers of the apoptotic cell were counted in the frontal lobes. The cell counts in the control group were 1.67(]SY.+-。[)1.00 (n=9) on E10,1.88(]SY.+-。[)0.99 (n=8) on E12,1.83(]SY.+-。[)1.17(n=6) on E14,1.44(]SY.+-。[)1.75(n=16) on E 16 and 1.56(]SY.+-。[)1.32(n=16) on E18, and in the infected group 3.63(]SY.+-。[)3.70(n=8), 3.50(]SY.+-。[)1.76 (n=6), 3.08(]SY.+-。[)2.11(n=12), 2.18(]SY.+-。[)2.40(n=11) and 1.00(]SY.+-。[)1.71(n=16), respectively. There were considerable differences among individual mice in both groups and statistically a low significance except on E12 (p<0.05). In conclusion an increased number of apoptotic cells in the infected brians could be preceded to that in the controls. This evedence suggests that cell damage might be resulted by a direct protozoal affection to in the fetal brain or by an ischemic influence from placentitis of the infected mother and, therefore, cortical hypoplasia would be induced.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (57 results)

All Other

All Publications (57 results)

  • [Publications] 田中 順一, 福田 隆浩: "Parkinson病における大脳基底核の神経病理-特にその責任病巣を中心に-" 日本臨牀. 55. 32-38 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中 順一: "多発性硬化症の病理" Clin Neurosci. 15. 160-164 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中 順一: "老年者の白質とは" 老年期痴呆. 11. 249-256 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中 順一: "視床下部-下垂体の病理" Clin Neurosci. 15. 1157-1261 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Obotani T, Asanuma M, Tanaka J, Takashima S: "Evidence of brain ischemia in early neonatal sudden death syndrome." Neuropediatrics. 28. 145-148 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 牧岡 朝夫: "トキソプラズマ" Today's Therapy. 21. 102-104 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J, Minamitani M, Takada K: "Congenital Muscular Dystrophies" Elsevier,Amsterdam, 9 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 田中 順一, 岩田誠: "グラフィック神経学" 医歯薬出版,東京, 219 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukuda T,Tanaka J.: "Parkinsonism and meurotoxic substances. Immunohistochemistry of 2-methyl-1,2,3,4-tetrahydroisoquinoline-treated mice (in Japanese)." Igaku No Ayumi (J Clin Exp Med). 172. 145-8 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukuda T,Tanaka J,Watabe K,Minamitani M.: "Granulo-reticular spheroid bodies appearing in various neurodegenerative diseases are immunohistochemically made up of neuronal components" Jikeikai Med J. 42. 87-92 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukuda T,Watabe K,tanaka J.: "An Immunohistochemical study of macrophages in spinal cord lesions of amyotrophic lateral sclerosis." Tokyo Jikeikai Ikadaigaku Zasshi (Tokyo Jikekai Med J). 111. 183-8 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Minamitani M,Tanaka J,Suzuki Y.: "Pathomechanism of cerebral hypoplasia in experimental toxoplasmosis in murine fetuses." Early Hum Dev. 44. 37-50 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mstsushima H,Bogenmann E.: "NGF induces terminal differentiation in trk-A expressing neuroblastoma cells." Prog Clin Biol Res. 385. 177-83 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makioka A,Ohtomo H.: "An increased DNA polymerase activity associated with virulence of Toxoplasma gondii." J Parasitol. 81(6). 1021-2 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Watabe K,Fukuda T,Tanaka J,Honda H,Toyohara K,Sakai O.: "Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth-promoting activities." J.Neurosci Res. 41. 279-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukuda T,Tanaka J.: "Pathology and treatment of parkinson's disease (in Japanese)." J Med Represent. 4. 1-5 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J.: "Nasu-Hakola's disease (in Japanese)." Brain Med. 7. 453-6 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] TAnaka J,Ueno E.: "Parkinson's disease in advanced ages (1) : its allied disorders with dementia (in Japanese)." Ronenki Chiho (J Sen Dement). 9. 123-6 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J,Ueno E.: "Parkinson's disease in advanced ages(2) : its allied disorders with dyskinesia (in Japanese)." Ronenki Chiho (J Sen Dement). 9. 219-22 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J,Suematsu N.: "Centenarian brain (in Japanese)." Ronenki Chiho (J Sen Dement). 9. 327-30 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fukuda T,Watabe K,Tanaka J.: "Effects of bromocriptine and /or L-DOPA on neurons in substantia nigra of MPTP-treated C57BL/6 mice." Brain Res. 728. 274-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Minamitani M,Tanaka J,Suzuki Y.: "Pathomechanism of cerebral hypoplasia in experimental toxoplasmosis in murine fetuses." Early Hum Dev. 44. 37-50 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J.: "Enlargement of the perivascular space in brain (in Japanese)." Clin Neurosci. 14. 610-1 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J.: "Pathology of multiple sclerosis (in japanese)." Clin Neurosci. 15. 160-4 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J,Fukuda T.: "Pathology of Parkinson's disease (in Japanese)." Prog Med. 16. 2768-72 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J,Fukuda T.: "Neuropathology of the basal ganglia in Parkinson's disease with a special reference to its responsible lesion (in Japanese)." Nihon Rinsho (Jpn J Clin Med). 55. 32-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J.: "Pathology of multiple sclerosis (in Japanese)." Clin Neurosci. 15(2). 160-4 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J.: "What happens in the cerebral white matter of the elderly ? (in Japanese)" Ronenki Chiho (J Sen Dement). 11. 249-56 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J.: "Pathology of the subthalamus and potuitary (in Japanese)." Clin Neurosci. 15. 1157-61 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Makioka S.: "Toxoplasma (in Japanese)." Today's Therapy. 21. 102-4 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J.: Demyelinating disease and parkinsonism (in Japanese). In : Kitamura Y,Aozasa K,eds. Introduction to New Pathology. Tokyo. Nanzan-do, 288-93 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J,Iwata M.: Tokyo : Ishiyaku. Color Atlas of Graphic Neurology (in Japanese)., 1-219 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka J,Minamitani M,Takada K.: Amsterdam : Elsevier. Brain Pathology in Fukuyama type congenital muscular dystrophy with special reference to the cortical dysplasia and the occurrence of neurofibrillary tangles. In : Fukuyama Y,Osawa M,Saito K,Eds. Congenital Muscular Dystrophies., 189-97 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Minamitani M,Tanaka J,Suzuki Y: "Pathomechanism of cerebral hypoplasia in experimental toxoplasmosis in murine fetuses" Early Hum Dev. 44. 37-50 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Minamitani M,Tanaka J,Maekawa K: "Peculiar eosinophilic inclusions within astrocytes in a patient with malformed brain" Brain Dev. 16. 309-314 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] 南谷幹之,田中順一,野崎秀次,前川喜平: "慢性炎症を伴い,小多脳回,異所性灰白質,脳梁欠損,クモ膜嚢胞がみられた重症心身障害児の1剖検例" 脳と発達. 25. 359-363 (1993)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fukuda T,Tanaka J,Watabe K,Minamitani M: "Granulo-reticular spheroid bodies appearing in various neurodegenerative diseases are immunohistochemically made up of neuronal components" Jikeikai Med J. 42. 87-92 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Obotani T,Asanuma M,Tanaka J,Takashima S: "Evidence of brain ischemia in early neonatal sudden death syndrome." Neuropediatrics. 28. 145-148 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 牧岡朝夫: "トキソプラズマ" Today's Therapy. 21. 102-104 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tanaka J,Minamitani M,Takada K: "Congenital Muscular Dystrophies" Elsevier,Amsterdam, 9 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 田中順一,岩田 誠: "グラフィック神経学" 医歯薬出版,東京, 219 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsushima H: "NGF induces terminal differentiation in trk-A expressing neuroblastoma cells." Prog Clin Biol Res. 385. 177-183 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Watabe K: "Mitogenic effects of platelet-derived growth factor,fibroblast growth factor,transforming growth factor-β,and heparin-binding serum factor for adult mouse Schwann cells." J Neurosci Res. 39. 525-534 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Watabe K: "Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth-promoting activities." J Neurosci Res. 41. 279-290 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukuda T: "2-Methyl-1.2.3.4-tetrahydroisoquinoline dose dependently reduce the number of lyrosine hydroxylase-immunoreactive cells in the substantia nigra and locus ceruleus of C57BL/6J mice." Brain Res. 639. 325-328 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukuda T: "Granulo-reticular spheroid bodies appearing in various neurodegenerative diseases are immunohistochemically made up of uronal components." Jikei Med J. 42. 87-92 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Minamitani M: "Pathomechanism of cerebral hypoplasia in experimintal toxoplasmosis in murine fetuses." Early Hum Dev. 44. 37-50 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田中順一: "小児神経学の進歩 第22集" 診断と治療社, 11 (1993)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田中順一: "脊椎脊髄損傷の治療" 現代医療社, 21 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsushima H: "NGF induces terminal differentiation in trk-A expressing neuroblastoma cells." Prog Clin Biol Res. 385. 177-183 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watabe K: "Mitogenic effects of platelet-derived growth factor, fibroblast growth factor, transforming growth factor-β, and heparin-binding serum factor for adult mouse Schwann cells." J Neurosci Res. 39. 525-534 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watabe K: "Spontaneously immortalized adult mouse Schwann cells secrete autocrine and paracrine growth-promoting activities." J Neurosci Res. 41. 279-290 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukuda T: "2-Methyl-1, 2, 3, 4-tetrahydroisoquinoline does dependently reduce the number of tyrosine hydroxylase-immunoreactive cells in the substantia nigra and locus ceruleus of C57BL/6J mice." Brain Res. 639. 325-328 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukuda T: "Granulo-reticular spheroid bodies appearing in various neurodegenerative diseases are immunohistochemically made up of uronal components." Jikeikai Med J. 42. 87-92 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Minamitani M: "Pathomechanism of cerebral hypoplasia in experimental toxoplasmosis in murine fetuses." Early Hum Dev. 44. 37-50 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 田中順一: "神経病理学-基礎と臨床-" 朝倉書店, 17 (1992)

    • Related Report
      1995 Annual Research Report
  • [Publications] 田中順一: "小児神経学の進歩 第22集" 診断と治療社, 11 (1993)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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