| Project/Area Number |
07680833
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Fujita Health University |
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Principal Investigator |
ARAI Ryohachi Fujita Health University, Anatomy, Professor, 医学部, 教授 (20159487)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Parkinoson's disease / Serotonin neuron / L-dopa / Dopamine / Aromatic L-amino acid decarboxylase / Monoamine oxidase / 若香族アミノ酸脱炭酸酵素 / 線条体 |
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| Research Abstract |
The aim of the present study is to examine by immunohistochemistry whether dopamine produced from L-dopa plus a peripheral decarboxylase inhibitor, carbidopa, a cluster of dopamine-immunoreactive neurons was found in the dorsal raphe nucleus (DR). In L-dopa/carbidopa-injected rats that were pretreated with an intraperitoneal injection of a MAO inhibitor, pargyline, whem compared with the L-dopa/carbidopa-injected rats without the pargyline pretreatment, neurons of the cluster of the DR became much darker in dopamine staining. The distribution of dopamine-stained neurons in the DR of these rats corresponded very closely to the previously reported distribution of serotonin-immmunoreactive neurons of normal rats. In normal or only pargyline-injected rats, dopamine-stained neurons were scarcely observed in the DR.We previously showed that serotonin neurons of the rat DR were induced to contain dopamine by the injection of L-dopa plus carbidopa. These findings suggest that the newly produced dopamine from L-dopa in serotonin neurons of the rat DR is degraded by endogenous MAO.
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