| Project/Area Number |
07680847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Kumamoto University |
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Principal Investigator |
YAMAMOTO Hideyuki Kumamoto University School of Medicine, Pharmacology, Assistant Professor, 医学部, 講師 (60191433)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUNAGA Kohji Kumamoto University School of Medicine, Pharmacology, Associate Professor, 医学部, 助教授 (90136721)
MIYAMOTO Eishichi Kumamoto University School of Medicine, Pharmacology, Professor, 医学部, 教授 (50109659)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Alzheimer's disease / Ca^<2+> / Calmodulin / Neuronal differentiation / CaM kinase II / Microtubule-associated protein 2 / PC12 cells / Antiphosphopeptide antibody / 細胞骨格蛋白質 / 蛋白質燐酸化酵素 / 分子生物学 |
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| Research Abstract |
1) In order to investigate the physiological roles of Ca^<2+>/calmodulin-dependent protein kinase II (CaM kinase II) in neuronal differentiation, we transfected the cDNA of the alpha subunit of mouse CaM kinase II into PC12 cells. We have established the subclonal cell lines that constitutively express the transfected CaM kinase II.The phosphorylation of microtubule-associated protein 2 (MAP2) in these cell lines was higher than in the wild type cells, and was enhanced by treatment with ionomycin. 2) The two antiphosphopeptide antibodies to tau indicate that tau in the peripheral nervous system and Alzheimer's disease brain is highly phosphorylated at serine 199, serine 202 and serine 396.3) We synthesized a peptide (MAP2 peptide) which included serine 1562 of MAP2. MAP2 peptide was strongly phosphorylated by CaM kinase II.4) We prepared antiphosphopeptide antibody which reacted with MAP2 phosphorylated at serine 1562 by CaM kinase II.5) The phosphorylation of MAP2 in cerebellar culture cells was enhanced by treatment with ionomycin.
These results suggest that CaM kinase II regulates the functions of MAP2 in the cells and that phosphorylation of tau is increased in Alzheimer's disease brain. The antiphosphopeptide antibody to MAP2 is useful for the investigation of phosphorylation of MAP2 by CaM kinase II in the cells.
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