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Enhancement of neural regeneration and neural survival in peripheral nervous system by Novel Liver Derived Neuronal Activator

Research Project

Project/Area Number 07680849
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurochemistry/Neuropharmacology
Research InstitutionYokohama City Univ., School of Medicine

Principal Investigator

HORIE Hidenori  Yokohama City Univ., School of Medicine, Lecturer, 医学部, 講師 (80046135)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordstissue culture / collagen gel / neural regeneration / adult rat / hepatocyte / enhancement factor / autonomic nerve / cell death / 迷走神経 / 交感神経 / 脊髄後根神経節
Research Abstract

Novel liver derived neuronal activator (LDNA) has been proved to enhance neurite regeneration from transected-nerve terminals of adult rat DRG with associated nerve bundles and support their survival. In this project we proved that LDNA promotes neuronal cell survival in adult rat DRG explant after in serum-free culture. After 6 days in culture the explants were rapidly frozen in liquid nitrogen and then sectioned by a cryostat with 10 micron thickness. Nissle bodies in the sections were stained with either Nissle method or Giemsa method. LDNA improved the relative number of Nissle-positive neurons to total neurons from 33% to 40%. These results suggest that LDNA improves neurite regeneration and their survival resulting in inhibition of neuronal cell death. We demonstrated another important function of LDNA that LDNA promotes neuronal regeneration from either vague nerve or > sympathetic nerve both of which contacts with liver. When LDNA was applied to the vagus nerve, this factor enhanced neurite regeneration from the transected-vagus nerve terminals in comparison with that in LDNA-free culture. Similar enhancement by LDNA was seen in neurite regeneration from transected-nerve terminals of superior cervical ganglion. These autonomic nerves are thought to play important roles in either metabolism or regeneration in liver. It is plausible that liver may secrete LDNA and activate either its metabolic function or regeneration capability by promoting autonomic nerve regeneration to contact liver itself.
On the other hand we advances the project to determine amino acid sequence of LDNA from 1000 L of hepatocyte conditioned medium. Purification of the factor is going well and it is expected that the amino acid sequence will be determined in an early opportunity. Then we will start to cloning the factor.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Sango,K.: "Biophysical and biochemjcal properties of diabetle neurons in culture;What do we learn about diabetic neuropathy?" J.Peripher.Sys.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horie,H.: "IL-1β enhances neurite regeneration from transected-nerve terminals of adult rat DRG" Neuro Report. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sango,K.: "Diabetes impairs DRG neuronal attachment to extracellular matrix proteins in VItro." Brain Res.Bull.37. 533-537 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zhang,H.: "Mitogen induced proliteration of isolated adult mouse Schwann cells." J.Meurosci.Res.41. 648-654

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 堀江秀典: "肝由来神経活性化因子" 精神神経薬理. 18. 875-883 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 堀江秀典: "視神経再生促進因子-肝由来神経活性化因子-" 神経眼科. 12. 288-292 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 堀江秀典: "肝由来神経栄養因子" CLINICAL NEUROSCI.13. 836-838 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horie, H., Sasaki, I., Akahori, Y.and Kadoya, T.: "IL-1beta enhances neurite regeneration from transected-nerve terminals of adult rat DRG" Neuro Report. 8 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sango, K., Horie, H.and Inoue, S.: "Biophysical and biochemical properties of diabetic neurons in culture : What do we learn about diabetic neuropathy?" J.Peripher.Sys.(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sango, K., Horie, H., Okamura, A., Inoue, S.and Takenaka, T.: "Diabetes impairs DRG neuronal attachment to extracellular matrix proteins in vitro" Brain Res Bull.37. 533-537 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zhang, H., Hikawa, H., Horie, H.and Takenaka T.: "Mitogen induced proliferation of isolated adult mouse Schwann cells" J.Neurosci.Res.41. 648-654 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horie, H.: "Liver derived neuronal activator" J.J.Neuropsychopharmacology. 18. 875-883 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horie, H.: "Enhancement factor of optic nerve regeneration -Liver derived neuronal activator" Neuro-ophthalmol.Jpn.12. 288-292 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Horie, H.: "Liver derived neurotrophic factor" Clinical Neurosci.13. 836-838 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.HORIE: "IL-1β enhances neurite regeneration from transected-nerve terminals of adult rat DRG" NeuroReport. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] "Biophysical and biochemical properties of diabetic neurons in culture : What do we learn about Diabetic Neuropathy?" J.Peripher.Sys.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 堀江秀典: "肝由来神経活性化因子" 精神神経薬理. 18. 875-883 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.TAKANO: "Neurotrophins enhance neurite outgrowth from adult rat retina in vitro." Soc.Neurosci.22. 998 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.HORIE: "Liver derived neuronal activator(LDNA)enhances neurite regeneration from agend human retina." Soc.Neurosci. 22. 744 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Zhang,B.T.: "Mitogen induced proliferation of isolated adult mouse Schwann cells" J.Neurosci.Res. 41. 648-654 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 堀江秀典: "肝由来神経栄養因子" Clinical Neuroscience. 13. 836-838 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Fukuda,N.: "Elasticity of mouse hepatocyte membraneis regulated by microfilaments." Yokohama Medical Bull.44. 185-190 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sango,K.: "Diabetes impairs,DRG neuronal attachment to extracellular matrix proteins in vitro" Brain Res.Bull.37. 533-537 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 堀江秀典: "視神経再生促進因子-肝由来神経活性化因子-" 神経眼科. 12. 288-292 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 堀江秀典: "老化神経再生促進・生存維持物質の探索-肝細胞由来神経活性化因子-" 横浜医学. 46. 708-710 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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