| Project/Area Number |
07680878
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | SAPPRORO MEDICAL UNIVERSITY |
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Principal Investigator |
AOKI Mamoru Sapporo Medical University, School of Medicine, Department of Physiology, Professor, 医学部, 教授 (50001871)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Yasumitu Sapporo Medical University, School of Medicine, Department of Physiology, Instru, 医学部, 助手
NAKAZONO Yoshimi Sapporo Medical University, School of Medicine, Department of Physiology, Instru, 医学部, 助手 (30180300)
SATOMI Hajime Sapporo Medical University, School of Medicine, Department of Physiology, Assist, 医学部, 講師 (10045445)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Gervical respiratory neuron / Phrenic motoneuron / Neurobiotin / WGA-HRP / Cat / 上部頸髄呼吸性ニューロン / 呼吸運動 |
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| Research Abstract |
Functional morphology of upper cervical respiratory neurons (UCINs) were studied in cats by use of electrophysiological and histological methods in the period from 1995,4 to 1997,3. Following important findings were obtained. In the first year, extracellular and intracellular recordings from UCINs in C_1-C_2 segments were carried out and UCINs were synchronized with phrenic nerve discharge (C_5 branch). In the following year, histologcial identification of UCINs was attempted. WGA-HRP solution (5%) was applied to the central cut-end of the phrenic nerve and retrograde transynaptically labeled UCINs were observed in the intermediate gray matter of C_1 and C_2 segments. Labelled UCINs were multipolar with soma diameters of 20-30mum. Also Neurobiotin was applied to UCINs by intophoresis near extracellular recording sites. They were similar to those of WGA-HRP labeled cells.
These results demonstrated funcional morphology of UCINs in cats.
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