| Project/Area Number |
07680911
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Niigata University |
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Principal Investigator |
FUJISAWA Nobuyoshi Niigata University, School of Medicine, Assistant, 医学部, 助手 (50199311)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Norimitsu L Niigata University, School of Medicine, Associate Professor, 医学部, 助教授 (00111716)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Mesenchymal Chondrosarcoma / Metastasis / Chinese Hamster / TGF-beta 1 / 軟骨肉腫 / 肺転移 / TGF-β_1 |
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| Research Abstract |
We made reseaches in the availability of clonal cell lines MCS-1 and MCS-8 from a chinese hamster spontaneous mesenchymal chondrosarcoma as an experimental model of cancer metastasis, and obtained the following some valuable findings.
1.Inoculation root and target organ : Both cells inoculated from the lateral tail vein of nude mice had high metastatic potential to lung. Only MCS-8 cells made pulmonary metastatic nodules by subcutaneous inoculation. It was considered as a model of spontaneous metastasis. From these results, the main target organ of them was considered to be lung.
2.Evaluation of experimental metastasis : By comparative study on the metastatic potentials of both the tumor cell lines, it was important to count the number of nodules at the stage when they grow as large as we can see under the dissecting microscope by isolating with the dissecting needles, because they have different growth rate in the metastasized organ.
3.Differences in susceptibility by strains or sexes of nude mice : CD-1 (closed colony) and BALB/c (inbred strain) nude mice were used. Both MCS-1 and MCS-8 made smaller number of nodules in the latter strain than in the former. But the metastatic potentials of the cell lines was same both in CD-1 and BALB/c, or males and females of the animals.
4.Effects of TGF-beta 1 inhibited experimental pulmonary metastasis of MCS-1 and cell growth in vitro, but no effect on in vivo growth rate and type ll collagen synthesis in MCS-1 was observed.
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