| Project/Area Number |
07680921
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Tokai University |
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Principal Investigator |
UEYAMA Yoshito Tokai University School of Medicine Associate Professor, 医学部, 助教授 (30072408)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Hitoshi Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (20191273)
NAKAMURA Masato Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (00164335)
TAMAOKI Norikazu Tokai University, School of Medicine, Professor, 医学部, 教授 (50055860)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | immune deficient mouse / lung cancer xenograft / metastasis / nm23 gene / 皮下移植 / NM23遺伝子 / 腫瘍Xenograft |
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| Research Abstract |
In this study, we established an experimental model for in vivo distant metastasis of human xenografts in severe combined immune deficient (SCID) mice. We examined non-metastatic (NM23) gene expression levels in the total 50 of various kinds of human tumor xenograts. We isolated human lung cancer xenografts LC-11JCK and LC-17JCK.The LC-17JCK did not express NM23 gene transcripts, whereas the LC-11JCK showed remarkable expression of NM23 gene. The xenogarafts were subcutaneously transplanted into the SCID mice. The mice were autopsied after transplantation to detect distant metastases into the lung or liver. We also examined the expression levels of vascular endothelial growth factor.
The human lung cancer xenograft LC-17JCK showed remarkable liver and lung metastases 3 months after transplantation. However, the LC-11JCK did not show any distant metastasis in the mice. We also examined the metastatic incidence of the LC-17 in the SCID/beig mice. The distant metastasis is significantly increased in the SCID/beige mice. The function of natural killer cells are additionally suppressed in the SCID/beige mice. The LC-17 xenografts also showed an increased level of VEGF gene transcripts.
These results suggest the expression levels of NM-23 gene and VEGF gene may related to distant metastases of the human lung cancer xenografts in vivo. This experimental model using severe combined immune deficient mice are potent system for the research of cancer metastasis.
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