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Comparative genome mapping of experimental animals by FISH

Research Project

Project/Area Number 07680926
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionNagoya University (1996)
National Institute of Radiological Sciences (1995)

Principal Investigator

MATSUDA Yoichi  Nagoya University, School of Agricultural Sciences, Associate Professor, 農学部, 助教授 (70165835)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsFISH / chromosome mapping / genome map / cDNA / mouse / rat / hamster / Suncus / 遺伝子マッピング / R-バンディング / DNAクローン / シリアンハムスター
Research Abstract

Fluorescence in situ hybridization (FISH) is an effective technique for localization of cloned DNA probes directly onto metaphase chromosomes. Human and mouse genome mapping using FISH has been significantly enhanced by development of new techniques, especially high-resolution gene mapping with direct R-banding FISH.
In this study we applied direct R-banding FISH to some map-poor experimental animals, rat, Syrian hamster and Suncus murinus, and established high-resolution sytogenetical mapping system for construction of genome map in these species. The hybridization efficiency of DNA clones on the replication R-banded chromosomes was sufficient for mapping of small sizes of cDNA clones and genomic DNA clones in all the species.
The advantage of FISH method allows direct mapping of mouse and human cDNA probes to choromosomes of different species. We tried chomosome mapping of functional genes in rat and Syrian hamster using mouse and human cDNA clones to construct comparative genome map in these species. We mapped 70 and 46 genes to rat and Syrian hamster chromosomes, respectively, and we could identified a new syntenic region between mouse Chromosome 13 and rat Chromosome 1, and new 17 syntenic regions between mouse and Syrian hamster chromosoes. We colud also map three functional genes to Suncus chromosomes with human, mouse and rat cDNA clones.
We found at least two chromosome inversions between mouse ans rat X chromosomes by comarative mapping with 21 mouse X chromosome-specific cDNA clones. This result suggests that comparative genome mapping between mouse and other rodent species is very useful to study genomic rearrangement on the process of evolution in the rodent species

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] Suzuki, H., Sakurai, S. & Matsuda, Y.: "Rat 5S rDNA spacer sequences and chromosomal assignment of the gene to the extreme terminal region of chromosome 19" Cytogenetics and Cell Gentics. 72. 1-4 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y. et al: "Chromosome mapping of human (ZNF179), mouse and rat genes for brain finger protein (bfp), a member of the RING finger protein" Genomics. 33. 325-327 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y. et al: "Comparative genome mapping of ataxia telangiectasia region in the mouse, rat and hamster" Genomics. 34. 347-352 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y. et al: "Comparative mapping of the reeler gene on human chromosome 7q22, rat chromosome 4q11.2 and mouse chromosome 5A3-B1" Mammalian Genome. 7. 468-469 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y. et al: "Chromosomal assignment of the Bdl2-related genes, Bcl2l abd Bax, in the mouse and rat" Cytogenetics and Cell Genetics. 74. 107-110 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada S., Okazaki, Y. & Matsuda, Y.: "Exclusion of candidate genes for hamster cardiomyopathy by FISH analysis" DNA Research. 3. 273-276 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 松田洋一(分担): "染色体のin situハイブリダイゼーション,「組織培養の技術第3版」" 朝倉書店, 11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y.and Chapman, V.M.: "Application of fluorescence in situ hybridization in genome analysis of the mouse." Electrophoresis. 16. 261-272 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Harada, Y,-N., Matsuda, Y.Shiomi, N.and Shiomi, T.: "Complementary DNA sequence and chromosomal localization of xpg, the mouse counterpart of human repair gene XPG/ERCC5." Genomics. 28. 59-65 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Honma, N., Matsuda, Y.Ishii, Y., Sato, M., Tomura, T., et al: "Molecular cloning and chromosomal localization of the mouse glycosylation inhibiting factor gene and four homologous genes." Mammal.Genome. 7. 51-59 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki, H., Sakurai, S.and Matsuda, Y.: "Rat 5S rDNA spacer sequences and chromosomal assignment of the gene to the extreme terminal region of chromscome 19." Cytogenet.Cell Gent.72. 1-4 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Nara, K., Watanabe, Y., Saito, T.and Sanai, Y.: "Chromosome mapping of the GD3 synthase gene in man and mouse." Genomics. 32. 137-139 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Inoue, S., Seki, N., Hosoi, T., Orimo, A., et al.: "Chromosome mapping of human(ZNF179), mouse and rat genes for brain finger protein(bfp), a member of the RING finger protein." Genomics. 39. 325-327 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Takahara, T., Kusakabe, M.and Hayashizaki, Y.: "Comparative mapping of the reeler gene on human chromosome 7q22, rat chromosome 4q11.2 and mouse chromosome 5A3-B1." Mammal.Genome. 7. 468-469 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Imai, T., Shiomi, T., Saito, T., Yamauchi.W., et al.: "Comparative genome mapping of ataxia telangiectasia region in the mouse, rat and hamster." Genomics.34. 347-352 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Enomoto, H., Saito, T.and Sakiyama, S.: "Chromosome mapping of the mouse and rat homologs of the human DAN gene, DlSl733E." Mammal.Genome. 7. 709-710 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Kusano, H.and Tsujimoto, Y.: "Chromosomal aasignment of the Bc12-related genes, Bcl2l abd Bax, in the mouse and rat." Cytogenet.Cell Genet.74. 107-110 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takada, S., Okazaki, Y., Kamiya, M., Ohsumi, T.and Matsuda, Y.: "Five candidate genes for hamster cardiomyopathy did not map to the cardiomyopathy locus by FISH analysis." DNA Research. 3. 273-276 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Hamatani, K., Itoh, M., Takahashi, E., Araki, R.and Abe, M.: "Localization of the importin-beta gene to mouse chromosome 11D and rat chromosome 10q32.1" Genomics. 36. 213-215 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Akao, Y.and Matsuda, Y.: "Identification and chromosome mapping of the mouse homologue of the human gene (DDX6) that encodes a putative RNA helicase of the DEAD box proteinfamily." Cytogenet. Cell Genet.75. 38-44 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Saito, T., Fujimoto, J.and Yamamoto, T.: "Chrmosomal mapping of the mouse and rat brt genes." Genomics. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuda, Y., Hori, T.and Morita, T.: "Chrmosome mapping of the mouse homologues of DMCl, the yeast meiosis-specific homologous recombination gene." Chromosome Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,H.,Sakurai,S.& Matsuda,Y.: "Rat 5S rDNA spacer sequences and chromosomal assignment of the gene to the extreme terminal region of chromosome 19" Cytogenetics and Cell Gentics. 72. 1-4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda,Y.et al: "Chromosome mapping of human (ZNF179),mouse and rat genes for brain finger protein (bfp),a member of the RING finger protein" Genomics. 33. 325-327 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda,Y.et al: "Comparative genome mapping of ataxia telangiectasia region in the mouse,rat and hamster" Genomics. 34. 347-352 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda,Y.et al: "Comparative mapping of the reeler gene on human chromosome 7q22,rat chromosome 4q11.2 and mouse chromosome 5A3-B1" Mammalian Genome. 7. 468-469 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda,Y.et al: "Chromosomal assignment of the Bc12-related genes,Bcl2l abd Bax,in the mouse and rat" Cytogenetics and Cell Genetics. 74. 107-110 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takada S.,Okazaki,Y.& Matsuda,Y.: "Exclusion of candidate genes for hamster cardiomyopathy by FISH analysis" DNA Research. 3. 273-276 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松田洋一(分担): "染色体のin situハイブリダイゼーション,「組織培養の技術 第3版」" 朝倉書店, 11 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Matsuda, Y. and Chapman, V. M.: "Application of fluorescence in situ hybridization in genome analysis of the mouse." Electrophoresis. 16. 261-272 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yen,C. -H., Matsuda, Y. et al.: "A genomic clone containing a telomere array maps near the centromere of mouse Chrmosome6." Mammalian Genome. 6. 96-102 (1955)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shimokawa, N., Matsuda, Y. and Yamaguchi,M.: "Genomic cloning and chromosomal assignment of rat regucalcin gene." Molecular and Cellular Biochemistry. 151. 57-163 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Harada, Y. -N., Matsuda, Y., Shiomi, N. and Shiomi, T.: "Complementary DNA sequence and chromosomal localization of xpg, the mouse counterpart of human repair gene XPG/ERCC5." Genomics. 28. 57-65 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] lnoue, S., Orimo, A., Matsuda,Y.,et al.: "Chromosomal mappings of human and mouse genes for estrogen-responsive finger protein (efp), a member of RING finger family." Genomics. 25. 581-583 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kaul, S.c., Wadhwa, R., Matsuda,Y. et al.: "Mouse and human chromosomal assignments of mortalin, a novel member of the murine hsp70 family of proteins." FEBS Letters. 361. 269-272 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 松田洋一: "染色体のin situハイブリダイゼーション,「組織培養の技術 第3版」" 朝倉書店(印刷中),

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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