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Study on Biomaterials for Drug Delivery Sysytem Based on Dynamic Structural Change of Molecular Assembly of Elastin Peptides

Research Project

Project/Area Number 07680946
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKyushu Institute of Technology

Principal Investigator

OKAMOTO Kouji  Kyushu Institute of Technology, Department of Biochemical Engineering and Science, Professor, 情報工学部, 教授 (40122618)

Co-Investigator(Kenkyū-buntansha) UEMURA Yuko  Kyushu Institute of Technology, Department of Biochemical Engineering and Scienc, 情報工学部, 助手 (80203488)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsElastin / Molecular assembly / Dynamic structural change / Drug delivery system
Research Abstract

<1995>A high polymer based on the repeating pentapeptide sequence Val-Pro-Gly-Val-Gly which has the property of salf-assembly, a peptide Arg-Gly-Tyr-Ser-Leu-Gly which can be phosphorylated by protein kinase, and two peptides Gly-Ser-Asn-His-Gly and Gly-Arg-Asn-Thr-Gly in which Asn residue can be converted to Asp residue, were synthesized. Arg-Gly-Tyr-Ser-Leu-Gly was phosphorylated by cyclic AMP dpendent protein kinase. The half-lives for Asn-Asp conversion were 6 days and 28 days for Gly-Ser-Asn-His-Gly and Gly-Arg-Asn-Thr-Gly.
<1996>Poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser-Leu-Gly)] which consists of the indicated molar ratios was synthesized. This polymer was phosphorylated by cyclic AMP dependent protein kinase for 24 hr at 30゚C.The resulting phosphorylated polymer poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser (PO_4^<2->) -Leu-Gly)] was studied for self-assembly. The temperature for the inverse temperature transition (Tm) of the phosphorylated polymer was shifted to higher temperature giving a shift of 14゚C.The phosphorylated polymer was incubated with alkaline phosphatase for 4 hr at 30゚C to yield a dephosphorylated poly [15 (Val-Pro-Gly-Val-Gly), (Arg-Gly-Tyr-Ser-Leu-Gly)]. Tm was shifted to lower temperature giving a shift of 13゚C.This result indicated complete dephosphorylation of phosphorylated polymer.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] 神里早月: "Involvement of intracellular cyclic GMP and cyclic GMP-dependent protein kinase in α-elastin induced macrophage chemotaxis" Journal of Biochemistry. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上村祐子: "The role of repeating peptide sequences of extracellular matrix elastin on cell proliferation and cell migration" Peptide Chemistry 1996. 329-332 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 甲斐原 梢: "Elastin peptide coacervate as a model of extracellular elastomeric matrix : jonic transport study" Peptide Chemistry 1996. 493-496 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上村祐子: "Elastin-derived peptide enduces monocyle chemotaxis by increasing intracellular cyclic GMP level and activating cyclic GMP dependent protein kinase" Biochem.Mol.Biol.Intl.41. 57-64 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 甲斐原 梢: "Characteristic interaction of Ca^<2+> ions with elastin coacervate : ion transport study across coacervate layers of α-elastin and elastin model polypeptide,(Val-Pro-Gly-Val-Gly)n" Biopolymers. 39. 189-198 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 上村祐子: "Elastin peptides induce monocyte chemotaxis by increasing the level of cyclic GMP,an intracellular second messenger" Peptides : Chemistry,Structure and Biology. 412-413 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 甲斐原 梢: "Biological self-assembly model of elastomeric protein : characterization of critical processes in coacervation of elastin peptides" Peptide Chemistry 1995. 345-348 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 児玉浩明: "Structural change in elastin model peptides upon interaction with biologically important metal ions" Peptide Chemistry 1995. 433-436 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 杉尾正則: "Coacervation properties of chemically modified elastin peptides as new functinality biomaterials" Peptide Chemistry 1994. 453-456 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 輪干浩史: "Stimulation of cell proliferation and autoregulation of elastin expression by elastin peptide VPGVG in cultured chick vascular smooth muscle cells" FEBS Letters. 368. 215-219 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 岡元孝二: "新タンパク質応用工学(旗野昌弘監修)" フジ・テクノシステム, 950 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 岡元孝二: "Data Book on Mechanical Properties of Living Cells,Tissues,and Organs (H.Abe,K.Hayashi,and M.Sato,eds.)" Springer, 429 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Sugio, Y.Uemura, K.Kaibara, M.Kondo, and K.Okamoto: "Coacervation Properties of Chemically Modified Elastin Peptides as New Functionality Biomaterials" Peptide Chemistry 1994 (M.Ohno, ed.), Protein Research Foundation. 453-456 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Wachi, Y.Seyama, S.Yamashita, H.Suganami, Y.Uemura, K.Okamoto, H.Yamada, and S.Tajima: "Stimulation of Cell Proliferation and Autoregulation of Elastin Expression by Elastin" Peptide VPGVG in Cultured Chick Vascular Smooth Muscle Cells FEBS Letters. 368. 215-219 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H.Kodama, Y.Higashimoto, S.Kajiyama, Y.Uemura, K.Kaibara, K.Okamoto, M.Jelokhani-Niaraki, and M.Kondo: "Structural Change in Elastin Model Peptides upon Interaction with Biologically Important Metal Ions" Peptide Chemistry 1995 (N.Nishi, ed.), Protein Research Foundation. 433-436 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.kaibara, K.Okamoto, K.Miyakawa, and M.Kondo: "Biological Self-Assembly Model of Elastomeric Protein : Characteristics of Critical Processes in Coacervation of Elastin Peptides" Peptide Chemistry 1995 (N.Nishi, ed.), Protein Reserach Foundation. 345-348 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Uemura, S.Kamisato, M.Arima, N.Takami, and K.Okamoto: "Elastin Peptides Induce Monocyte Chemotaxis by Increasing the Level of Cyclic GMP" Peptides : Chemistry, Structure and Biology (P.T.P.Kaumaya and R.S.Hodges, ed.), Mayflower Scientific Ltd.412-413 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kaibara, T.Akinari, K.Okamoto, Y.Uemura, S.Yamamoto, H.Kodama, and M.Kondo: "Characteristic Interaction of Ca^<2+> Ions with Elastin Coaceravates : Ion Transport Study Across Coaceravate Layrs of alpha-Elastin and Elastin Model Polypeptides. (Val-Pro-Gly-Val-Gly) n" Biopolymers. 39. 189-198 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Okamoto, Y.Murakami: "DaI15Setu Koaserubashonn to Tannpakusitukinouseigyo" Sinntannpakusituouyoukougaku(M.HatanoKannsuu), Fuji.Tekunosisutemu. 478-486 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Okamoto: "4.2.Biological Proteins and Polymers Data Book on Mechanical Properties of Living Cells, Tissues, and Organs (H.Abe, K.Hayashi, and M.Sato, eds.)" Springer. 383-409 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Uemura, S.Kamisato, and K.Okamoto: "The Role of Repeating Peptide Sequences of Extracellular Matrix Elastin on Cell Proliferation and Cell Migration" Peptide Chemistry 1996 (C.Kitada, ed.), Protein Reserach Foundation. 329-332 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Kaibara, K.Okamoto, and M.Kondo: "Elastin Peptide Coacervate as a Model of Extracellular Elastomeric Matrix : Ionic Tranport Study" Peptide Chemistry 1996 (C.Kitada, ed.), Protein Reserach Foundation. 493-496 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Uemura and K.Okamoto: "Elastin-Derived Peptide Induces Monocyte Chemotaxis by Increasing Intracellular Cyclic GMP Level and Activating Cyclic GMP Dependent Protein Kinase" Biochem.Mol.Biol.Int.41 (1). 57-64 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S.Kamisato, Y.Uemura, N.Takami, and K.Okamoto: "Involvement of Intracellular Cyclic GMP and Cyclic GMP-Dependent Protein Kinase in alpha-Elastin -Induced Macrophage Chemotaxis" J.Biochem.121 (5) (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 神里早月: "Involvement of intracellular cyclic GMP and cyclic GMP-dependent protein kinase in α-elastin induced macrophage chemotaxis" Journal of Biochemistry. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上村(山下)祐子: "The role of repeating peptide sequences of extracellular matrix elastin on cell proliferation and cell migration" Peptide Chemistry 1996. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 甲斐原 梢: "Elastin peptide coacevate as a model of extracellular elastomeric matrix : ionic transport study" Peptide Chemistry 1996. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上村(山下)祐子: "Elastin-derived peptide induccs monocyte chemotaxis by increasing intracellular cyclic GMP level and activating cyclic GMP dependent protein kinase" Biochem.Mol.Biol.Intl.41. 57-64 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 神里早月: "Characteristic interaction of Ca^<2+> ions with elastin coacervate : ion transport study across coacervate layers of α-elastin and elastin model polypeptide,(Val-Pro-Gly-Val-Gly)n" Biopolymers. 39. 189-198 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 上村祐子: "Elastin peptides induce monocyte chemotaxis by increasing the level of cyclic GMP,an intracellular second messenger" Peptides : Chemistry,Structure & Biology. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 甲斐原 梢: "Biological self-assembly model of elastomeric protein : characterization of critical processes in coacervation of elastin peptides" Peptide Chemistry 1995. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 児玉浩明: "Structural change in elastin model peptides upon interaction with biologicallly important metal ions" Peptide Chemistry 1995. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 杉尾正則: "Coacervation properties of chemically modified elastin peptides as new functionality biomaterials" Peptide Chemistry 1994. 453-456 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 輪干浩史: "Stimulation of cell proliferation and autoregulation of elastin expression by elastin peptide VPGVG in cultured chick vascular smooth muscle cells" FEBS Letters. 368. 215-219 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岡元孝二: "新タンパク質応用工学(旗野昌弘監修)" フジ・テクノシステム, 950 (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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