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Enzymatic polymevization in reversed micelles

Research Project

Project/Area Number 07804045
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 機能・物性・材料
Research InstitutionUniversity of Shizuoka

Principal Investigator

GOTO Ayako  School of Administration and Informatics associated Prof., 経営情報学部, 助教授 (80046284)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordsreversed micelle / poly (A) / polymerization / Nano-structure / AFM / metal ion / solid / Liquid interface / polynucleotidephosphate / Polynucleotide phosphorylase / 固 / 液界面 / 核酸 / 重合反応 / 原子間力顕微鏡(AFM) / クラスター / polyA / PNPase / water pool / ナノ構造体 / ガラス表面 / 生体高分子
Research Abstract

1) The dynamic state of sodium bis (2-ethylhexyl) sulfosuccinate (AOT) reversed micellar solution was studied by NMR.Concerning the state of water and AOT at small water content, I proposed a new model. Furthermore, AOT reversed micelles was visually observed by freeze-fracture and Cryo- transmission electron microscopy. The AOT reversed micelles were observed visually at first and the flocculation of AOT reversed micelles was direcly proved.
2) The enzymatic polymerizaiton of ADP was carried out in AOT reversed micellar solutions containing Mg^<2+> ion. The poly (adenylic acid) being formed in the water pools precipitated out of the AOT solution together woth the enzyme, whose activity wa maintained for a long time. The mechanism of the precipitation was studied. The growning process of the precipitate aggregates was observed by atomic force microscopy.
3) We tried the enzymatic polymerization in the presence of Fe^<3+> ion in stead of Mg ^<2+> ion. The polymerization was not proceeded in AOT solution, but it was proceeded in nonionic reversed micellar solution of heptaoxyethylene dodecylether. Furthermore, the precipitation was also accelerated as temperature increased.
4) The polymerization of ADP in the presence of metal ion in cationic surfactant reversed micelles without enzymes was stuied. We are examing the product by capillary electrophoresis.
In conclusion, the polymerizaiton of nucleic acid in reversed micellar solution was of interest from the viewpoint of chemical engineering as well as of origin of life.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 五島 綾子: "化学的自己創出系の構築" 表面. 35. 31-40 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 五島 綾子: "逆ミセル溶液の構造と機能" 液膜研究会誌. 32. 7-14 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Goto, H.Yoshioka, M.Manabe, R.Goto: "NMR Spectroscopy Study on the Dissolution of Water in Sodium Bis (2-ethylhexyl) Sulfosuccinate/Toluene Solution" Langmuir. 11. 4837-4875 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Goto, Y.Kuwahara, A.Suzuki, H.Yoshioka, R.Goto, T.Iwamoto: "Flocculation of Sodium Bis (2-ethylhexyl) Sulfosuccinate Reversed Micelles in lsooctane" J.Mol. Liquid. 72. 137-144 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Goto, H.Hakamata, Y.Kuwahara, R.Goto, P.Walde, P.L.Luisi, and T.Imae: "Functional Nano-Structure of Aggregate Self-Organized on the Liquid/Solid Interface-Enzymatic Polymerization of ADP-" Progr.Colloid Polym Sci. 106. 245-248 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Goto, R.Goto.Y.Kuwahara, H.Hakamata, P.Walde, P.L.Luisi, and T.Imae: "AFM Observation of Poly (A) Proliferated on the Liquid/Solid Interface from Sodium Bis (2-ethylhexyl) Sulfosuccinate/Isooctane Reversed Micellar Solution" Langmuir. (submittied).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A.Goto, T.Imae, R.Goto., .Walde, and P.L.Luisi: "Visual Observation of Aerosol OT Reversed Micelles nad Enzymatic Synthesized Poly (adenylic acid) in Reversed Micelles." Chemical Engeneering. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A. Goto et al.: "NMR spectrospoic study on the dissolution of water in sodium bis (2-ethylhexyl) sulfosuccinate/toluene solution" Langmuin. 11. 4873-4875 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 五島綾子: "化学的自己創出系の構築" 表面. 35. 31-40 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] A. Goto et al.: "Flocculation of sodium bis (2-ethylhexyl) sultosuccinate reversed micelle in isooctne" Journal of Moleular Liquid. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] A. Goto et al.: "Visual Obsercation of Aerosol OT Reversed micelles and enzymatic synthesized poly (adenylic acid) in reversed micelles" Chemical. Engineering. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 五島綾子: "逆ミセルの構造と機能" 液膜研究会誌. (印刷中).

    • Related Report
      1996 Annual Research Report
  • [Publications] 五島綾子(分担): "21世紀へのものづくりにむけて-分子化学から自己組織化へ-" 編者小島茂,文眞堂, 26 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Goto H.Yoshioka.M.Manabe and R.Goto: "NMR Spectro soopic study on the pissolution of Water in sodium Bis(2-ethylhexyl)Sulfosuccinatel Toluene Solution" Langmuir. 11. 4873-4875 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 五島綾子・中垣正幸: "化学的自己創出系" 表面. (予定). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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