• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Phylogenetic relationship of Galliform subfamilies by hemoglobin

Research Project

Project/Area Number 07804061
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 系統・分類
Research InstitutionUniversity of the Ryukyus

Principal Investigator

EGUCHI Yukinori  University of the Ryukyus Faculty of Medicine Research Laboratory Center, Assistant Professor, 医学部, 助教授 (50160354)

Co-Investigator(Kenkyū-buntansha) KAYO Susumu  University of the Ryukyus Faculty of Medicine Research Laboratory Center, Assist, 医学部, 助手 (30233993)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsHemoglobin / Amino Acid Sequence / Molecular Evolution / Phylogenetic Tree / キジ亜科 / キジ科
Research Abstract

Phylogenetic relationships of Galliform subfamilies analyzed by morphological classification, DNA hybridization, Lysozyme c amino acid sequence data and cytochrome b nucleic acid sequence data. However, these results were showed the different phylogenetic trees. We approach phylogenetic relationship of subfamilies of Galliforms by hemoglobin amino acid sequence data. In the first plan, it considered that the acquirement of the avian blood was difficult, it planned to use PCR.The blood of California quail and Guinea fowls could be luckily, acquired. It considered that we got the amino acid sequence data of some spices of Galliforms. We determined the hemoglobin amino acid sequence of California quail and Guinea fowls. The amino acid sequence data of some spices of Galliforms, added two new data of California quail and Guinea fowls, analyzed by maximum likelihood method and neighbor joining method. As a result, phylogenetic tree from hemoglobin data resembled tree from the mitochondria DNA data and DNA hybridization. These two trees were reversed the relationship between California quail and Guinea fowls. In parallel of these study, we developed the PCR application for the rare species which difficult to get the blood. We improved a new method of DNA extraction from the feather which got high yield as the conventional method. Recently, we extracted DNA from the chicken feather and the PCR reaction can amplify of globin gene.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 江口 幸典: "キジ亜科ヘモグロビンの分子進化について" 生化学. 67. 724- (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 池原 強: "ハトα^Dグロビン遺伝子のクローニング" 生化学. 68. 1772- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 池原 強: "ハトα^Dグロビン遺伝子の構造解析" 生化学. 68. 1090- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 江口 知子: "エラブウミヘビのグロビン遺伝子構造の解析" 生化学. 68. 1090- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 江口 幸典: "シギダチョウヘモグロビンの一次構造について" 生化学. 68. 627- (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yukinori Eguchi: "Molecular evolution of Galliform subfamilies by hemoglobin" Seikagaku. 67. 724 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tuyoshi Ikehara: "Cloning of pigeon a^D-globin gene" Seikagaku. 68. 1772 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tuyoshi Ikehara: "Structure analysis of pigeon a^D-globin gene" Seikagaku. 68. 1090 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomoko Eguchi: "Structure analysis of Erabu sea snake a-globin gene" Seikagaku. 68. 1090 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yukinori Eguchi: "Amino acid sequence of Tinamou hemoglobin" Seikagaku. 68. 627 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 江口幸典: "キジ亜科ヘモグロビンの分子進化について" 生化学. 67. 724 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 池原 強: "ハトα^D-グロビン遺伝子のクローニング" 生化学. 68. 1772- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 池原 強: "ハトα^D-グロビン遺伝子の構造解析" 生化学. 68. 1090- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 江口知子: "エラブウミヘビのαグロビン遺伝子構造の解析" 生化学. 68. 1091- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 江口幸典: "シギダチョウヘモグロビンの一次構造について" 生化学. 68. 627- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 江口幸典: "キジ亜科ヘモグロビンの分子進化について" 生化学. 67. 724 (1994)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi