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Simultaneous Measurement of Photosynthetic Electron Transport and CO_2 uptake for the Analysis of Physiological Function of Tree Leaves.

Research Project

Project/Area Number 07806023
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 林学
Research InstitutionKyushu University

Principal Investigator

KOBAYASHI Yoshichika  Kyushu Univ., Dept.of Forestry, Asso.Prof., 農学部, 助教授 (90087594)

Co-Investigator(Kenkyū-buntansha) YAHATA Hisashi  Kyushu Univ., Inst.of Tropical Agri., Prof., 熱帯農学研究センター, 教授 (90038290)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsChlorophyll fluorescence / Electron transport / Photorespiration / Photosynthesis / Quantum yield / Intact leaves
Research Abstract

The purpose of this research was to develop a method for estimating rates of electron transport in intact leaves by the simultaneous measurement of quantum yield and CO_2 uptake. Quantum yield of photosynthesis was determined from the fluorescence parameter (Fm'-Fs) /Fm', where (Fm'-Fs) was defined as the difference between steady state chlorophyll fluorescence (Fs) and maximum fluroescence (Fm') elicited by a saturating light pulse. The rate of electron transport through photosystem II (total electron flow) was calculated from the product of quantum yield and A (PFD), where A is the rate of absorbed photons as given by leaf absorptance, and PFD is the photon flux density at the leaf surface. The rate of electron transport dependent on CO_2 uptake (assimilative electron flow) was calculated from the gross photosynthetic rate in a leaf. The difference between the rates of total and assimilative electron transport was denoted as the rate of non-assimilative electron transport which depends on photorespiration and oxygen reduction. Available data provided quantitative information on the rate of non-assimilative electron flow in intact leaves. We also found that tree leaves with low rates of photosynthesis have greater activities of photorespiration than the other plant leaves with high rates of photosynthesis. The method for estimating the rate of electron transport in intact leaves provided useful information for the analyzes of photorespiration and photosynthesis limitation in tree leaves.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Kobayashi,Y.: "Coupling ratios H^+/e=3 versus H^+/e=2 in chloroplasts and quantum requirements of net oxygen exchange during the reduction of nitrite,ferricyanide or methylviologen." Plant Cell Physiol.36(8). 1613-1620 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi,Y.: "Bioenergetics of carbon assimilation in intact chloroplasts : Coupling of proton to electron transport at the ratio H^+/e=3 is incompatible with H^+/ATP=3 in ATP synthesis." Plant Cell Physiol.36(8). 1629-1637 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Heber,U.: "Maximum H^+/hνPSII stoichiometry of proton transport during cyclic electron flow in intact chloroplasts is at least two,but probably higher than two." Plant Cell Physiol.36(8). 1639-1647 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Heber,U.: "Coupled cyclic electron transport in intact chloroplasts and leaves of C3 plants : Does it exist? If so,what is its function?" Photosynth.Res.,. 46. 269-275 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibata,M.: "Hydrogenation of chlorophyll alcohol side chain and its role on photosynthesis." Plant & Cell Physiol.III. 997-1000 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyama,M.: "Simultaneous measurements of quantum yield and CO_2 uptake for the assessment of non-assimilative electron flow in tree leaves" J.Forest Res.1(2). 79-85 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi, Y., Neimanis, S.and Heber, U.: "Coupling ratios H^+/e=3 versus H^+/e=2 in chloroplasts and quantum requirements of net oxygen exchange during the reduction of nitrite, ferricyanide or methylviologen." Plant Cell Physiol. 36(8). 1613-1620 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi, Y., Kaiser, W.and Heber, U.: "Bioenergetics of carbon assimilation in intact chloroplasts : Coupling of proton to electron transport at the ratio H^+/e=3 is incompatible with H^+/ATP=3 in ATP synthesis." Plant Cell Physiol. 36(8). 1629-1637 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Heber, U., Bukhov, N.G., Neimanis, S.and Kobayashi, Y.: "Maximum H^+/hvPSII stoichiometry of proton transport during cyclic electron flow in intact chloroplasts is at least two, but probably higher than two." Plant Cell Physiol. 36(8). 1639-1647 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Heber, U., Gerst, U., Krieger, A., Neimanis, S.and Kobayashi, Y.: "Coupled cyclic electron transport in intact chloroplasts and leaves of C3 plants : Does it exist? If so, what is its function?" Photosynth.Res.46. 269-275 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shibata, M., Tsuyama, M., Kitagawa, Y.and Kobayashi, Y.: "Hydrogenation of chlorophyll alcohol side chain and its role on photosynthesis." Photosynthesis (P.Mathis ed.). Vol.III. 997-1000 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tsuyama, M., Kobayashi, H., Shinya, M., Yahata, H.and Kobayashi, Y.: "Simultaneous measurements of quantum yield and CO_2 uptake for the assessment of non-assimilative electron flow in tree leaves" J.Forest Res.1(2). 79-85 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kobayashi,Y.: "Rates of vectorial proton transport supported by cyclic electron flow during oxygen reduction by illuminated intact chloroplasts" Photosynth.Res.41. 419-428 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Heber,U.: "Coupled cyclic electron transport in intact chloroplasts and leaves of C3 plants:Does it exist?lf so,what is its function?" Photosynth.Res.46. 269-275 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi,Y.: "Coupling ratios H^+/e=3 versus H^+/e=2 in chloroplasts and quantum requirements of net oxygen exchange during the reaction of nitrite,ferricyanide or methylviologen" Plant & Cell Physiol.36. 1613-1620 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi,Y.: "Bioenergetics of carbon assimilation in intact chloroplasts:Coupling of proton to electron transport at the ratio H^+/e=3 is incompatible with H^+/e=3 in ATP synthesis" Plant & Cell Physiol.36. 1639-1647 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Heber,U.: "Maximum H^+/hυ_<PSI> stoichiometry of proton transport during cyclic electron flow inintact chloroplasts is at least two,but probably higher than two" Plant & Cell Physiol.36. 1639-1647 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tsuyama,M.: "Simultaneous measurements of quantum yield and CO_2 uptake for the assessment of non-assimilative electron flow in tree leaves" J.Forest Res.1. 79-85 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kobayashi, Y.: "Rates of vectorial proton transport supported by cyclic electron flow during oxygen reduction by illuminated intact chloroplasts" Photosynth. Res.41. 419-428 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Heber, U.: "Coupled cyclic electron transport in intact chloroplasts and leaves of C3 plants : Does it exist? If so, what is its function?" Photosynth. Res.46. 269-275 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi, Y.: "Coupling ratios H^+/e=3 versus H^+/e=2 in chloroplasts and quantum requirements of net oxygen exchange during the reaction of nitrite, ferricyanide or methylviologen" Plant & Cell Physiol.36. 1613-1620 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi, Y.: "Bioenergetics of carbon assimilation in intact chloroplasts : Coupling of proton to electron transport at the ratio H^+/e=3 is incompatible with H^+/e=3 in ATP synthesis" Plant & Cell Physiol. 36. 1629-1637 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kobayashi, Y.: "Maximum H^+/hυ_<PSI> stoichiometry of proton transport during cyclic electron flow in intact chloroplasts is at least two, but probably higher than two" Plant & Cell Physiol.36. 1639-1647 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsuyama, M.: "Simultaneous measurements of quantum yield and CO_2 uptake for the assessment of non-assimilative electron flow in tree leaves" J. Forest Res.1 (in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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