• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Functional and physiological analysis of plechstrin homology domain structure

Research Project

Project/Area Number 07807019
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionFukui Medical School

Principal Investigator

INAZU Tetsuya  Fukui Medical School, Research associate, 医学部, 助手 (00242587)

Co-Investigator(Kenkyū-buntansha) MARUYAMA Shingo  Fukui Medical School, Research associate, 医学部, 助手 (90262633)
NOGUCHI Tamio  Fukui Medical School, Professor, 医学部, 教授 (70135721)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Keywordspleckstrin homology / pleckstrin cDNA cloning / protein kinase C / genome DNA cloning / リン脂質
Research Abstract

Dr.Mayr found that there were similar about 100 amino acid sequences in various signaling molecules, which have divergent function. He called this domain as pleckstrin homology (PH) one, and proposed that this domain wouldbe important motif on "Cell " journal at 1993. When I planned this project, only 6 proteins which contained PH domain were reported. However, since researchers recognized PH domain as valuable, the number of PH containing protein rapidly increased.
My plan was 1) molecular cloning of chicken pleckstrin cDNA,2) molecular cloning of genomic pleckstrin DNA,3) gene knockout in chicken B lymphocyte DT40 and functional analysis. At first, I tried to molecular cloning of chicken pleckstrin cDNA.In consequence of several efforts, I took several cDNA clones and analyzed them. Positive clone contained an 1,059 bp open reading frame encoding a polypeptide of 353 residues sharing 81% overall identity with the human pleckstrin. Then N- and C-terminal PH domain was also conserved ; 89% and 79%, respectively. The length of chicken pleckstrin's mRNA was about 3kb and this expression was restricted to spleen and DT40 B cell line. Then, using this cDNA as a probe, I took several genomic clones. But they are too complicated to analyze, I could not completely finish this No.2) project. Therefore I could not undertake the plan 3) gene knockout in chicken B lymphocyte DT40 and functional analysis.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Suofu Qin: "Activation and tyrosine phosphorylation of p72syk as well as calcium mobilization after hydrogen peroxide stimulation in peripheral blood" Biochem. J.308. 347-352 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroyuki Maeda: "Possible involvement of protein-tyrosine kinase such as p72syk in discsphere change response of porcine platelets." J. Biochem.117 (6). 1201-1208 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suofu Qin: "Cooperation of tyrosine kinase p72syk and p53/56 lyn regulates calcium mobilization in chicken B cell oxidant stress signalling." Eur. J. Biochem.236. 443-449 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suofu Qin, Tetsuya Inazu, and Hirohei Yamamura: "Activation and tyrosine phosphorylation of p72syk as well as calcium mobilization after hydrogen peroxide stimulation in peripheral blood lymphocytes." Biochem.J.308. 347-352 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hiroyuki Maeda, Tetsuya Inazu, katsuya Nagai, Shingo Maruyama, Gizou Nakagawara, and Hirohei Yamamura: "Possible involvement of protein-tyrosine kinase such as p72syk in disc-sphere change response of porcine platelets." J.Biochem.117(6). 1201-1208 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suofu Qin, Tetsuya Inazu, Minoru Takata, Tomohiro Kurosaki, Yoshimi Homma, and Hirohei Yamamura: "Cooperation of tyrosine kinase p72syk and p53/56 lyn regulates calcium mobilization in chicken B cell oxidant stress signalling" Eur.J.Biochem.236. 443-449 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suofu Qin: "Cooperation of tyrosine kinase p72syk and p53/56 lyn regulates calcium mobilization in chicken B cell oxidant stress signalling." Eur.J.Biochem.236. 443-449 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suofu Qin: "Activation and tyrosine phosphorylation of p72^<syk> as well as calcium mobilization after hydrogen peroxide stimulation in peripheral blood lymphocytes." Biochem.J.308. 347-352 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hiroyuki Maeda: "Possible involvement of protein-tyrosine kinase such as p72^<syk> in disc-sphere change response of porcine platelets." J.Biochem.117(6). 1201-1208 (1995)

    • Related Report
      1995 Annual Research Report

URL: 

Published: 1995-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi