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Calcitonin receptor polymorphism-function and diseases-

Research Project

Project/Area Number 07807023
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionWakayama Medical College, 2nd Department of Pathology

Principal Investigator

KAKUDO Kennichi  Wakayama Medical College, 2nd Department of Pathology, 第2病理学, 教授 (00112037)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordscalcitonin / calcitonin receptor / gene polymorphism / mammary carcinoma / receptor / race
Research Abstract

There are three isoforms of calcitonin receptors (CTRs). The first type (type 1) has 465 amino acids in rat and 468 amino acids in porcine. The second type (type 2) has a 39 amino acid insertion in the second extracellular domain. The third type (type 3) has a 16 amino acid insertion in the first intracellular domain. The human CTR which has been isolated from a human ovarian carcinoma cell line belongs to this type 3.
Using RT-PCR with primers whose sequences correspond to the human CTR cDNA,we analyzed the expression of the CTR transcripts in seven human tumor cell lines and surgical specimens. The CTR m-RNA were found in all samples. Transcripts which were 48bp shorter than that of the type 3 were detected, but not transcripts of type 2 and type 3. Since the structure of this CTR is same as that of the type 1, we termed it the human type 1 CTR.PCR studies using human genomic DNA as a template recvealed that the 48bp sequence constitutes an exon. These results indicate that the type 1 and the type 3 human CTRs are generated by alternative splicing and a majority of human CTR transcripts is type 1.
Structural analysis of CTRs from ten cultured human tumor cell lines and 117 human blood samples demonstrated allelic variants at the 1377th nucleotide in the intracellular domain 4, expressing either proline or leucine as the 463th amino acid. It was found that the variant with proline at this site was the more prevalent type of CTR among Japanese population.
The allele frequencies of the calcitonin receptor (CTR) gene were compared between 46 breast cancer patients and 50 controls. The allele frequencies of CTR gene did not differ between them. There was no significant association of CTR allele frequencies histologicaly in invasive breast carcinomas. These results indicate that allelic variants of CTR gene is not significant risk factor to the development of breast carcinoma in Japanese.
Further studies are needed to clear the function of CT or CTR in mammary tissue.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] M.Nakamura,K.Kakudo et al.: "Allelic variants of the Japanese population" Human Genet. 99. 38-41 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Z Zhang,K.Kakudo et al.: "Late occurrence of K-ras gene mutations in the pathogeneses of squamous cell carcinoma of the lung:Analysis in sputum." Anal Quant Cytol Hist. 18(6). 501-502 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T Hisa,K Kakudo et al.: "Scrotal Angiokeratoma in a young man." Acta Derm Venereol. 76. 75-76 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] N Matsuura,K Kakudo et al.: "Induction of experimental bone metastasis in mice by transfection of Integrin α4β1 into tumor cells." Am J Pathol. 148(1). 55-61 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Morikawa,K Kakudo et al.: "Expression of fibroblast growthfactor receptor-1 in human normal tissues and tumors determined by a new monoclonal antibody." Arch Pathol Lab Med. 120. 490-496 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] A Matsuno,K Kakudo et al.: "Clinical and histopathological analysis of proliferative petentials of recurrent and non-recument meningiomas." Acta Neuropathol. 191. 504-510 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura M,Hashimoto T,Nakajima T,Ichii S,Furuyama J,Ishihara Y,Kakubo K: "A new type of human calcitonin receptor isoform generated by alternative splicing" Biochem Bioph Res Comm. 209(2). 744-751 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsubayashi S,Kawai K,Matsumoto Y,Mukuta T,Morita T,Hirai K,Matsuzuka F,Kakudo K,Kuma K,Tamai H: "The correlation between papillary thyroid carcinoma and lymphocytic infiltration in the thyroid gland." J Clin Endocrinol Metab. 80. 3421-3424 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Iwasaki A,Shan L,Kawano I,Nakamura M,Utusnomiya H,Kobayashi A,Kuma K,Kakudo K: "Quantitative analysis of stromal fat content of human parathyroid glands associated with thyroid disease using computer image analysis." Pathol Int. 45. 483-486 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Utsunomiya H,Shan L,Kawano I,Iwasaki A,Ono K,Kobayashi A,Kuma K,Kishikawa S,Kakudo K: "Immunolocalization of parathyroid hormone in human parathyroid gland with special references to microwave antigen retrieval." Endoc Pathol. 6. 223-227 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morikawa Y,Tohya K,Ishida H,Matsuura N,Kakudo K: "Different migration patterns of antigen-presenting cells correlate with Th1/Th2-type responses in mice." Immunolgy. 85. 575-581 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morikawa Y,Ishihara Y,Matsuura N,Miyamoto H,Kakudo K: "Malignant Schwannoma of the liver" Dig Dis Sci. 40. 1279-1282 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shan L,Ishihara Y,Nakamura K,Jing X,Kawano I,Koujimoto Y,Kakudo K: "Signet ring cell carcinoma with varied malignat components in the urinary bladder-A case report with immunohistochemical study-." Wakayama Med Rep. 36. 45-49 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morikawa Y,Ishihara Y,Kawano I,Matsuura N,Kaname A,Kakudo K: "Cystic squamous cell carcinoma of the thyroid : Apossiblenew subgrorp of intrathyroidal epithelial thymoma." Endoc Pathol. 6(1). 77-81 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura M,Hashimoto T,Furuyama J,Kakudo K: "Multiple alternative splice isoforms of parathyroid hormone-related peptide mRNA in human cell lines." J Mol Endcrinol. 15. 245-249 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morikawa Y,Tohya K,Ishida H,Matsuura N,Kakudo K: "Differnt migration patterns of antigen-pressenting cells wrrelate with Th1/Th2-type responses in mice." Immunology. 85. 575-581 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shan L,Iwasaki A,Utsunomiya H,Kawano I,Matsuura N,Kobayashi A,Kuma K,Kakudo K: "Immunoreactive characteristics and classification of hyperparathyroidism." Endoc Pathol. 6(2). 145-152 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Morikawa Y,Ishihara Y,Tohya K,Kakudo K,Kurokawa, Seo M,Matsuura N: "Expression of fibroblast growth factor receptor-1 in human normal tissues and tumors determined by a new monoclonal antibody." Arch Pathol Lab Med. 120. 490-496 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuura N,Puzon-McLaughlin W,Irie A,Morikawa Y,Kakudo K,Takada Y: "Induction of experimental bone metastasis in mice by transfection of Integrin alpha 4 beta 1 into tumor cells." Am J Pathol. 148(1). 55-61 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsuno A,Fujimaki T,Asai A,Nagashima T,Ide T,Asai A,Matsuura R,Utsunomiya H,Kakudo K: "Clinical and histopathological analysis of proliferative petentials of recurrent and non-recument meningiomas." Acta Neuropathol. 91. 504-510 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hisa T,Goto Y,Taniguchi S,Nakanishi T,Kakudo K,Takigawa M: "Signs, Syndoromes and Diagnoses Post-bullous milia." Australian J Dermatol. 37. 153-154 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yamaue H,Tanimura H,Ishimoto K,Morikawa Y,Kakudo K: "Nodular lymphoid hyperplasia of the terminal Ileum. Report of a case and the findings of an immunological analysis." Jap J Surg. 26. 431-434 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hisa T,Taniguchi S,Kakudo K,Ichihashi M,Takashima T,Kato Y,Hayakawa R,Takigawa M: "Establishment of an in vitro cell derived from human angiosarcoma" Bull Cancer. 83. 589-591 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hisa T,Taniguchi S,Goto Y,Teramae H,Osato K,Kakudo K,Takigawa M: "Scrotal Angiokeratoma in a young man." Acta Derm Venereol. 76. 75-76 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Zhang Z,Nakamura M,Taniguchi E,Shan L,Yokoi T,Kakudo K: "Late occurrence of K-ras gene mutations in the pathogeneses of squamous cell carcinoma of the lung : Analysis in sputum." Anal Quant Cytol Hist. 18(6). 501-502 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hisa T,Taniguchi S,Nishimura T,Nakanishi T,Wakasa K,Kakudo K,Hirachi Y,Takigawa M: "Mobile fat ; three stage?" Australian J Dermatol. 37. 223-224 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Nakamura M,Zhang Z,Shan L,Hisa T,Sasaki M,Tsukino R,Yokoi T,Kaname A,Kakudo K: "Allelic variants of human calcitonin receptor in the Jaoanese population." Human Genet. 99(1). 38-41 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M.Nakamura, K.Kakudo et al: "Allelic variants of the Japanese population." Human Genet. 99. 38-41 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Z Zhang, K.Kakudo et al.: "Late occurrence of K-ras gene mutations in the pathogeneses of squamous cell carcinoma of the lung : Analysis in sputum." Anal Quant Cytol Hist. 18(6). 501-502 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T Hisa, K Kakudo et al.: "Scrotal Angiokeratoma in a young man." Acta Derm Venereol. 76. 75-76 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] N.Matsuura, K Kakudo et al.: "Induction of experimental bone metastasis in mice by transfection of Integrin α4 β1 into tumor cells." Am J Pathol. 148(1). 55-61 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y Morikawa, K Kakudo et al.: "Expression of fibroblast growth factor receptor-1 in human normal tissues and tumors determined by a new monoclonal antibody." Arch Pathol Lab Med. 120. 490-496 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A Matsuno, K Kakudo et al.: "Clinical and histopathological analysis of proliferative petentials of recurrent and non-recument meningiomas." Acta Neuropathol. 191. 504-510 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Misa Nakamura Kennichi Kakudo,etal.: "A new type of human calcitonin receptor isoform generated by alternative splicing" Biochemical and Biophysical Research Communications. 209(2). 744-751 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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