| Project/Area Number |
07807024
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Shinshu University |
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Principal Investigator |
OGISO Yoshifumi Shinshu University, School of Medicine, Assistant, 医学部附属病院, 助手 (50233440)
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| Co-Investigator(Kenkyū-buntansha) |
KUZUMAKI Noboru Hokkaido University, School of Medicine, Professor, 医学部, 教授 (80091445)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | ras oncogene / placenta / trophoblast / Na^+-K^+-ATPase / hCG / active transport / dome formation / choriocarcinoma |
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| Research Abstract |
During an implantation period of pregnancy, normal trophoblasts behave like malignant cells showing rapid growth capacity and invasiveness into the uterine endometrium. Probably a variety of oncogenes contribute to those malignant-like phenotypes of the normal trophoblasts. Since human choriocarcinoma cells generally preserve the diverse characterictics of normal trophoblasts, we transfected the viral H- or K-ras oncogene into a a human choriocarcinoma cell line, CC1, and analyzed the biological properties of CC1 cells expressing an activated ras oncogene. All H-ras-expressing clones distinctively formed the hemisherical domes, but none of K-ras-expressing clones and control clones did. Microscopic observation demonstrated that those domes were cavities filled with fluid which accumulated between the cell layr and the surface of culture dish. Scanning electron microscopy revealed that the dome cells were morphologicaly similar to a blastcyst. Furthermore, Na^+-K^+-ATPase activity activity was significantly higher in the H-ras-expressing clones. Those domes flattened within 24 hours after treatment with a specific inhibitor of Na^+-K^+-ATPase, ouabain. Next, we examined the production of human chorionic gonadotropin (hCG). K-ras-expressing clones secreted significantlt more hCG than H-ras-expressing clones and control cells.
In the present study, we showed that ras oncogenes are profoundly involved in the active transport and the hCG secretion of trophoblasts.
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