| Project/Area Number |
07807036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Institute of Basic Medical Science, Tsukuba University |
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Principal Investigator |
KOITO Atsushi Institute of Basic Medical Science, Tsukuba University, Lecturer, 基礎医学系, 講師 (70231305)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAUCHI Hiromitsu Institute of Basic Medical Science, Tsukuba University, Professor, 基礎医学系, 教授 (40175485)
廣近 玲 通産省工業技術院, 産業技術融合領域研究所, 特別技術補助職員 (90260223)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Retrovirus / Tropism / Envelope / HTLV-I / HIV-vector / レトロウィルス / HTLV-1 / レセプター / 組換えレトロウイルス / Pseudotype / パッケージング |
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| Research Abstract |
We have developed the system by which HIV genome carrying GFP is transduced into nucleus of target cells via through HTLV-I envelope (Koito et al., manuscript in preparation). In addition to conventional GFP, HIV vector carrying mutated GFP which excites at 395 nm (violet) is under construction. Using these systems, the target cell population to HTLV-I infection will be clarified. The cDNA library for the isolation of the gene coding for the HTLV-I receptor has been constructed from the human osteosarcoma (HOS) cells by using murine retrovirus vector. High titer recombinant retrovirus generated by transfection of cDNA library into packaging BOSC cell line (Kitamura et al., Proc.Natl.Acad.Sci.USA 92.1995) will be inoculated into the mouse NIH3T3 cells which is shown to be resistant to HTLV-I infection. This approach will be employed to identify the factor (s) involved in HTLV-I infection.
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