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Analysis of human interferon resistant genes of herpes simplex virus

Research Project

Project/Area Number 07807037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNational Institute of Health

Principal Investigator

ARAO Yujiro  National Institute of Health, Dept. of Pathology, Senior Researcher, 感染病理部, 主任研究官 (40151146)

Co-Investigator(Kenkyū-buntansha) ANDO Yasutaka  Keio University Medical School, Dept. of Ophtalmology, Researcher, 医学部・眼科学教室, 助手 (90193119)
IWASAKI Takuya  National Institute of Health, Dept. of Pathology, Chief Researcher, 感染病理部, 室長 (90146027)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
KeywordsHerpes simplex virus type 1 / Herpes simplex virus type 2 / Human interferon alpha / Human interferon beta / Human interferon gamma / interferon resistance / Intertypic recombinant virus / Marker rescue
Research Abstract

To better understand how herpes simplex virus (HSV) counteracts anti-HSV effects of interferons (IFNs), first, the sensitivity of 19 HSV strains to type I and II IFNs was compared in the infection processes of cultured human epithelial cells. There was good correlation between the sensitivities to the IFNs although they have different anti-HSV actions. This correlation was not due to induction of endogenous type I IFN by type II IFN treatment, because a neutralizing antibody to IFN beta did not diminish the anti-HSV effects of type II IFN.A similar correlation also was observed in human embryonic lung fibroblast cells. These results suggest that HSV has an ability to overcome the anti-HSV actions of both types of IFNs independently but with similar sensitivity.
Secomd, we examined complementation and recombination between two interferon (IFN) sensitive strains, 17syn of herpes simplex virus type 1 [HSV-1 (17syn)] and UW268 of HSV type 2 [HSV-2 (UW268)]. Double infection with the two str … More ains resists reduction in virus yield by IFN.Intertypic recombinants with restored IFN resistance were obtained from progeny viruses in the doubly-infected cultures. These results indicate that IFN resistance of HSV is controlled by at least two genetic regions.
Third, restriction endonuclease analysis of the intertypic recombinants deduced that one of the HSV coding regions for IFN resistance locates in BamHI-A,BglII-N or the neighboring fragments of HSV-2. Four cloned fragments, Bam HI-A and-E and BglII-I and-N,of an IFN resistant HSV-2 strain, G[HSV-2 (G)] were tested for their abilities to rescue IFN resistance of HSV-2 (UW268), resulting in that only BglII-N incresed plating efficiency of progeny viruses in co-transfected cultures. An IFN resistant HSV-2 clone was obtained from the co-transfected culture with BglII-N of HSV-2 (G) and the HSV-2 clone contained a DNA sequence within the fragment. Therefore, it is concluded that one of the HSV coding regions for IFN resistance locates in the BglII-N fragment of HSV-2. Less

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Y Arao,Y Ando,M Narita and T Kurata: "A similar sensitivity of herpes simplex virus infection to type I and II interferons" Journal of Interferon and Cytokine Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Ando,T Iwasaki,T Sata,S Soushi,T Kurata,Y Arao: "Enhanced cytopathic effect of human cytomegalovirus on a retinal pigment epithelial cell line,K-1034,by serum-free medium" Archives of Virology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J Padilla.M Yamada,Y Takahashi,T Tsukazaki.J Nakamura,M Yoshida.F Uno,Y Arao,S Nii: "In Vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes" Microbiology and Immunology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 山口優樹、荒尾雄二郎、倉田毅、栗村啓、川名尚、山西弘一、森島恒雄、本田まりこ、新村眞人、本藤良、吉田正己: "DNA診断による単純ヘルペスウイルスの型判定" 臨床とウイルス. 24. 63-68 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 荒尾雄二郎: "単純ヘルペスウイルス潜伏感染化の制御因子" Molecular Medicine. 33. 1444-1445 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 荒尾雄二郎、新居志郎: "単純ヘルペスウイルスの持続感染成立機構" 医学のあゆみ. 177. 857-863 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Arao, Y.Ando, M.Narita and T.Kurata: "A similar sensitivity of herpes simplex virus infection to type I and II interferons." Journal of Interferon Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Ando, T.Iwasaki, T.Sata, S.Soushi, T.Kurata, Y.Arao: "Enhanced cytopathic effect of human cytomegalovirus on a retinal pigment epithelial cell line, K-1034, by serum-free medium." Archives of Virology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] J Padilla, M Yamada, Y Takahashi, T Tsukazaki, J Nakamura, M Yoshida, F Uno, Y Arao, S Nii: "In Vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes." Microbiology and Immunology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Yamaguchi, Y.Arao, T.Kurata, T.Kurimura, H.Kawana, K.Yamanishi, T.Morishima, M.Honda, M.Niimira, R.Hondo, M.Yoshida: "Typing of herpes simplex virus by polymerase chain reaction." Rinsho to Uirusu. 24. 63-68 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Arao: "Factors regulating establishment of herpes simplex virus lateny." Molecular Medicine. 33. 1444-1445 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y.Arao, S.Nii: "Mechanism of establishment of herpes simplex virus latency." Igaku no ayumi. 177. 857-863

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Arao,Y Ando,M Narita and T Kurata: "A similar sensitivity of herpes simplex virus infection to type I and II interferonns" Journal of Interferon and Cytokine Research. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Y Ando,T Iwasaki,T Sata,S Soushi,T Kurata,Y Arao: "Enhanced cytopathic effect of human cytomegalovirus on a retinal pigment epithelial cell line,K-1034,by serum-free medium" Archives of Virology. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] J Padilla,M Yamada,Y Takahashi,T Tsukazaki,J Nasamura,M Yoshida,F Uno,Y Arao,S Nii: "In Vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes" Microbiology and Immunology. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 山口優樹、荒尾雄二郎、倉田毅、栗村敬、川名尚、山西弘一、森島恒雄、本田まりこ、新村眞人、本藤良、吉田正己: "DNA診断による単純ヘルペスウイルスの型判定" 臨床とウイルス. 24. 63-68 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 荒尾雄二郎: "Molecular Medicine" 単純ヘルペスウイルス潜伏感染化の制御因子, 1444-1445 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 荒尾雄二郎、新居志郎: "医学のあゆみ" 単純ヘルペスウイルスの持続感染成立機構, 857-863 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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