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Analysis of anti-tumor effect of HGF in transgenic mice

Research Project

Project/Area Number 07807057
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionTottori University

Principal Investigator

SHIOTA Goshi  Second Department of Internal Medicine, Tottori University School of Medicine, Assistant Professor, 医学部・附属病院, 助手 (70263457)

Project Period (FY) 1995 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
KeywordsHGF / hepatocellular carcinoma / anti-tumor effect / liver metastasis / double transgenic mice / 肝細胞癌 / 肝転移
Research Abstract

Since we developed transgenic mice expressing HGF in liver (G.Shiota et al.Hepatology 19,962-972,1994), we have studied anti-tumor effect of HGF by using transgenic mice. First, we studied metastatic potentials of hepatocellular carcinoma (HCC) cells in chimeric mice of HGF transgenic and nude. Second, we have developed 2 kinds of double transgenic mice of HGF with TGFalpha and c-myc, and have evaluated effect of HGF on TGFalpha-or c-myc-induced hepatocarcinogenesis.
1. Metastatic potential of HCC cells, such as HUH7, Hep3B and FaO cells, via portal vein was greatly suppressed in HGF/nude chimeric mice compared to nude control mice (81% vs.35%, p<0.05). 2. Double transgenic mice of TGFalpha and HGF developed HCC in 33% of mice, whereas TGFalpha mice developed HCC in 60% of mice. 3. Although c-myc mice developed adenoma and HCC in 92% and 58% of mice, HGF/c-myc double transgenic mice did 33% and 0%, respectively.

Report

(4 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] G.Shiota et al.: "Down-regulation of c-myc mRNA after complection of liver regeneration in transgenic nice expressing hepatocyte growth factor in liver." Res.Commun.Mol.Pathol.Pharmacol.89. 259-268 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Characterization of double transgenic nice expresing hepatocyte growth factor and transforning growth factor alpha" Res.Commun.Mol.Pathol.Pharmacol.90. 17-24 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Inhibitory effect of hepatocyte growth factor on metastasis of hepatocellular carciuana in transgenic nice" Res.Commun.Mol.Pathol.Pharmacol.91. 33-39 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Rapid induction of hepatocyle growth factor mRNA after adriristration of gnrisin A,a lignau component of schizandra fruit" Res.Commun.Mol.Pathol.Pharmacol.94. 141-146 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Santon-Rugi et al.: "Inhibition of neoplastic development in the liver by hepatocyte growth factor in a transgenic model" Proc.Natl.Acad.Sci USA. 93. 9577-9582 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Okano et al.: "Protective action of hepatocyte growth factor for acute liver inpiry caused by O-galaitosonire in transgenic nice." Hepatology. 26. 1241-1249 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Down-regulation of c-myc mRNA after completion of liver regeneration in transgenic mice expressing hepatocyte growth factor in liver." Res.Commun.Mol.Pathol.Pharmacol.89. 259-268 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Characteristics of double transgenic mice expressing hepatocyte growth factor and transforming growth factor alpha" Res.Commun.Mol.Pathol.Pharmacol.90. 17-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Inhibitory effect of hepatocyte growth factor on metastasis of hepatocellular carcinoma in transgenic mice" Res.Commun.Mol.Pathol.Pharmacol.91. 33-39 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Rapid induction of hepatocyte growth factor mRNA after administration of gomisin A,a lignan component of schizandra fruit" Res.Commun.Mol.Pathol.Pharmacol.94. 141-146 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] E.Santon-Rugi et al.: "Inhibition of neoplastic development in the liver by hepatocyte growth factor in a transgenic model" Proc.Natl.Acad.Sci.USA. 93. 9577-9582 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Okano et al.: "Protective action of hepatocyte growth factor for acute liver injury caused by D-galactosamine in transgenic mice" Hepatology. 26. 1241-1249 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Shiota et al.: "Down-regulation of c-myc mRNA after completton of liver regeneration in transgenic mice expressing hepatocyte growth factor in liver" Res.Commun.Mol.Pathol.Pharmacol.89. 259-268 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.Shiota et al.: "Characterization of double transgenic mice expressing hepatocyte growth factor And transforming growth factor alpha" Res.Commun.Mol.Pathol.Pharmacol.90. 17-24 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.Shiota et al.: "Inhibitory effect of hepatocyte growth factor on meta stasis of hepatocellular carcinoma in transgenic mice" Res.Commun.Mol.Pathol.Pharmacol.91. 33-39 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.Shiota et al.: "Rapid induction of hepatocyte growth factor mRNA after administration of gomisin A,a lignan conponent of cchizandra fruit" Res.Commun.Mol.Pathol.Pharmacol.94. 141-146 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Santon-Rugi et al.: "Inhibition of neoplastic development in the liver by hepatocyte growth factor in a transgenic model" Rroc.Natl.Acad.Sci.USA. 93. 9577-9582 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.Okano et al.: "Protective action of hepatocyte growth factor for acute liver injury caused by D-galactosanine in transgenic mice" Hepatology. 26. 1241-1249 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] G.Shiota et al: "Characterization of double transgenic mice expressing hepstoyte growth factor and transforming growth factorα" Res.Commun.Mol.Pathol.Pharmacal.90. 17-24 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] G.Shiota et al: "Inhibitory effect of hepatoyte growth factor on metartasis of hepatocellabar carcinoma in transgenic nice" Res.Commun.Mol.Pathol.Plarmacol. 91. 33-39 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] E.Santon-Rugi et al: "Inhibition of neoplastic development in the liver by hepatoyte growth factor in a transgenic mouse model." Proc.Natl.Acad.Sci USA. 93. 9577-9582 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shiota,G.et al: "Inhibitory effect of hepatocyte growth factor on metastasis Ω hepatocellular carcinoma cells" Res Commun Mol Pathol Pharnacol.91(in press). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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