NEW THERAPEUTIC APPROACH OF ANTIGEN-SPECIFIC IMMUNOTHERAPY FOR MYASTHENIA GRAVIS USING A COMPLEMENTARY PEPTIDE
| Project/Area Number |
07807065
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | TOTTORI UNIVERSITY |
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Principal Investigator |
ARAGA Shigeru TOTTORI UNIVERSITY,FACULTY OF MEDICINE,ASSOCIATE PROFESSOR., 医学部, 助教授 (40193065)
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| Co-Investigator(Kenkyū-buntansha) |
岸本 昌宏 鳥取大学, 医学部・附属病院, 助手 (40273891)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | COMPLEMENTARY PEPTIDE / EXPERIMENTAL AUTOIMMUNE MYASETHENIA GRAVIS / T-CELL RECEPTOR / TCR / 重症筋無力症 |
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| Research Abstract |
I HAVE SHOWN THAT IMMUNIZATION WITH A PEPTIDE TERMED RHCA 67-16 SPECIFIED BY RNA COMPLEMENTARY TO THAT ENCODING ACHR 61-76 CAN INDUCE POLYCLONAL AND MONOCLONAL ANTI-IDIOTYPIC (ID) ANTIBODIES (AB) WHICH RECOGNIZE THE PARATOPE OF ACHR 61-76 SPECIFIC AB AND ALSO BLOCK THE DEVELOPMENT OF EAMG.SINCE THE PRODUCTION OF SUCH DISEASECAUSING ACHR-SPECIFIC AB IS T-CELL DEPENDENT,WE HAVE TESTED WHETHER A PEPTIDE (DENOTED RHCA 611-001) SPECIFIED BY RNA COMPLEMENTARY TO THAT ENCODING A T CELL EPITOPE (ACHR 100-116 IN LEWIS RAT EAMG) COULD INDUCE AB WHICH RECOGNIZED RECEPTORS ON T CELLS (TCR) SPECIFIC FOR ACHR 100-116 AND WOULD THUS ALSO BLOCK THE DISEASE.POLYCLONAL AND MONOCLONAL (MAB) INDUCED BY IMMUNIZATION WITH RHCA 611-001 SPECIFICALLY BLOCKED THE PROLIFERATION OF TORPEDOACHR-SENSITIZED LYMPHOCYTES,STIMULATED WITH ACHR 100-116. FURTHERMORE A MAB AGAINST RHCA 611-001 ALSO IMMUNOPRECIPITATED A CELL SURFACE PROTEIN WITH THE CHARACTERISTICS OF A TCR FROM A ACHR 100-116 SPECIFIC T CELL LINE.ANALYSIS OF VB UASAGE OF EAMG SHOWED A LIMITED HETEROGENITY (VB 15) IN EAMG.SOME OF THE CLONED HAD A HIGH HOMOLOGY SEQUENCE BETWEEN THE CDR 3 REGIN OF TCR AND RHCA611-001.
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Report
(3 results)
Research Products
(7 results)
