| Project/Area Number |
07807108
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
YAMAMOTO Tetsu Asahikawa Med.Col., Assistant Professor, 医学部, 講師 (50125415)
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| Co-Investigator(Kenkyū-buntansha) |
澤 雅之 旭川医科大学, 医学部, 助手 (70226059)
KASAI Shinichi Asahikawa Med.Col., Associate Professor, 医学部, 助教授 (40091566)
ONODERA Kazuhiko Asahikawa Med.Col., Assistant (00204264)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Cord Blood / Hepatocyte Growth Factor (HGF) / Artificial Liver Support / Stem Cell |
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| Research Abstract |
The mononuclear cells in cord blood were studied as a source of liver support unit.
Initially most effective method of high yield on CD34 positive cells was discussed. The choice of anti-coaglant agent was the most important factor on the recovery of CD34 positive cells. Heparin was not effective because its short anti-coaglant activities. Thus the ACD solution was recomended for harvesting coad blood.
The serum level of HGF were 0.494<plus-minus>0.117ng/ml in cord blood (n=4) and 0.358<plus-minus>0.047ng/ml in peripheral blood (n=4). The released HGF levels in the culture medium (n=5) with cytocaines were 0.126<plus-minus>0.094ng/10^6 cells on 24 hours and 0.448<plus-minus>0.424ng/10^6 cells on 72 hours. And without cytocaines (n=5), the levels were 0.125<plus-minus>0.080ng/10^6 cells and 0.152<plus-minus>0.121ng/10^6 cells, respectively. The calculated HGF release values of CD34+CD33-cells with cytocaines were 13.3<plus-minus>11.3ng/10^6 cells on 24 hours and 41.2<plus-minus>31.7ng/10^6 cells on 72 hours.
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