| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Research Abstract |
Clinicals study on the concentration by using a new ion chromatography of ionized trace elements and electrolytes in body fluids of patients with gastrointestinal disease.
A new technique of ionchromatography was established for the simultaneous measurement of ionized trace elements (Fe^<2+>, Zn^<2+>, Mn^<2+>), electrolytes (Ca^<2+>, Mg^<2+>) and NH_<4+> in body fluids (serum, bile, pancreatic juice and urine). Among patients with gastrointestinal disease, Mg^<2+> concentration in serum was noticed to increase significantly in patients with hepatolithiasis (p<0.05).
Although total concentration of zinc in serum of patients with liver dysfunction was lower than in normal subjects, serum Zn^<2+> concentration was higher (p<0.05). Therefore, the ionized ratio of zinc was found to elevate significantly. There was a negative correlationship between the concentration of Zn^<2+> and serum albumin level (r=0.62) or serum prealbumin level (r=0.73). The concentration of Ca^<2+> elevated significantly in bile of patients with calcium bilirubinate stone. The concentration of Mg^<2+> slightly elevated. It was confirmed that Ca^<2+> and Mg^<2+> showed a correlationship with total bile acid. In particular, Mg^<2+> was found to have a correlation with chenodeoxycholic acid. It was suggested that the bile acid might be excreted by binding with Mg^<2+> into the bile.
In conclusion, a new technique of ion chromatography may be useful for analysis of humoral ion levels whith can be applied to evaluate liver functions and diagnose as gastrointestinal diseases.
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