| Project/Area Number |
07807135
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
HIRAYAMA Takakazu Asahikawa Medical College, Orthopedics, assistant professor, 医学部, 助教授 (10110644)
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| Co-Investigator(Kenkyū-buntansha) |
KATSUKI Masatoshi Asahikawa Medical College, Orthopedics, lecturer, 医学部, 助手 (60271772)
ATSUTA Yuji Asahikawa Medical College, Orthopedics, lecturer, 医学部, 講師 (90167924)
渡壁 誠 旭川医科大学, 医学部, 助手 (70182946)
竹光 義治 旭川医科大学, 医学部, 教授 (00038663)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥300,000 (Direct Cost: ¥300,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | fetal spinal cord / transplantation / sciatic nerve / motor unit / electromyography / fluorescein laveliing / nerve regeneration |
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| Research Abstract |
When motor neurons in the spinal cord are destroyed, regeneration of motor axons and muscle reinnervation cannot be expected. We attempted reinnervation of the denervated muscle, i.e., motor unit reconstruction, using transplantation of the fetal spinal cord to the peripheral nerve. The sciatic nerve of an adult rat was resected for 20mm, and a cavity was prepared using an autologous femoral vein at the distal stump of the nerve. The fetal spinal cord was then transplanted into the venous cavity. After 3-6 months, no voluntary muscle contraction was observed due to the absence of communication with the central nervous system. However, reinnervation of the muscles via the sciatic nerve by the transplanted spinal neurons was demonstrated electrophysiologically and histochemically. This suggested that a motor unit can be reconstrcted by fetal spinal cord transplantation even if the original motor neurons in the spinal cord are not available
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