PATHOGENESIS OF IDIOPATHIC SCOLIOSIS

• Project/Area Number: 07807137
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthopaedic surgery
• Research Institution: SHINSHU UNIVERSITY
• Principal Investigator: KAMIMURA Mikio (1996) SHINSHU UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部整形外科, 助手 (60273091); 大塚 訓喜 (1995) 信州大学, 医学部, 助教授
• Co-Investigator(Kenkyū-buntansha): HIDAI Yohei SHINSHU UNIVERSITY SCHOOL OF MEDICINE,ASSISTANT, 医学部整形外科, 助手 (50273090)
• Project Period (FY): 1995 – 1996
• Project Status: Completed (Fiscal Year 1996)
• Budget Amount: ¥1,800,000 (Direct Cost: ¥1,800,000); Fiscal Year 1996: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: idiopathic scoliosis / growth hormone / estrogen / estrogen

Research Abstract

The purpose of this study is to investigate the pathgenesis of idiopathic scoliosis. We hypothesized that scoliosis was sometime arised in growth, but deformity was useally reduced by natural correction force. We concidered that decrease of this natural correction gorce make deformity to true scoliosis, and it's related to balance of sex hormone and growth hormone.
Rat's tail were fixted into looped shape, 6 weeks later fixation was removed. After removing of fixation, the tail of rat were remained looped shape deformity, and a deformity was garadually decresed. We concidered these course of decreasing deformity as a model of natural correction of scoliosis.
We used 4 weeks male, female and ovarectomied (OVX) rat. These models were divided 2 groups and devided 6 subgroups, C : control (FC : female, CM : male, CO : OVX), G : growth hormone treated 17ug*3/W,2weeks (GF,GM,Go).
Two and 4 weeks after fixation, deformity of tail were examined in radiograph. Deformity were mesured within 5 verte brae and 4 disc by Cobb's methods, and wedge of disc were caliculated by total of 4 disc wedge angles.
The dosage of GH was not affected development of weight. The curve of tail was 144.9ﾟ ]SY.+-.[16.4 in CM group, 137.7ﾟ ]SY.+-.[23.5 in CF,143.5ﾟ ]SY.+-.[18.6 in CO,after 2 weeks, each was 93.3ﾟ ]SY.+-.[40.1,42.8ﾟ ]SY.+-.[9.6 (p<0.001 vs. CM), 34.4ﾟ ]SY.+-.[9.2 (p<0.001 vs. CM), and after 4 weeks 84.2ﾟ ]SY.+-.[43.0,44.1ﾟ ]SY.+-.[17.6 (p<0.001 vs. CM), 40.3ﾟ ]SY.+-.[7.9 (p<0.001 vs. CM). The disc wedge angles was 96.5ﾟ ]SY.+-.[11.2 in CM group, 85.6ﾟ ]SY.+-.[19.1 in CF,96.4ﾟ ]SY.+-.[12.8 in CO,after 2 weeks, each was 47.5ﾟ ]SY.+-.[21.7,24.7ﾟ ]SY.+-.[10.3 (p<0.001 vs. CM), 21.1ﾟ ]SY.+-.[9.0 (p<0.001 vs. CM), and after 4 weeks 58.5ﾟ ]SY.+-.[31.9,28.3ﾟ ]SY.+-.[15.0 (p<0.001 vs. CM), 27.1ﾟ ]SY.+-.[7.7 (p<0.001 vs. CM).
Compaired with C group and GH group, only in OVX group the siginificant difference was observed each 2 and 4 weeks (p<0.05).
This results indicate that the balance of sex hormone and growth hormone have a possibility to affect the worsening of idiopathic scoliosis.