| Project/Area Number |
07807139
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Orthopaedic surgery
|
|---|
| Research Institution | Shiga University of Medical Science |
|---|
Principal Investigator |
USHIYAMA Toshio Shiga University of Medical Science School of Medicine, Assistant, 医学部, 助手 (10263055)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
INOUE Koji Shiga University of Medical Science School of Medicine, Lecturer, 医学部, 講師 (80213156)
|
|---|
| Project Period (FY) |
1995 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥800,000 (Direct Cost: ¥800,000)
|
|---|
| Keywords | chondrocyte / estrogen receptor / IL-6 / TGF-beta / Chordrocyte / IL-6 / Estrogen receptor / Progesterone receptor / Andragen receptor / Synpvialcells / Chordrosyte / Osteoarthritis / Rhdumntoid orthrrtis / Androgen receptor / mRNA / osteoarthritis |
|---|
| Research Abstract |
1)Expression of sex hormone receptors in human articular chondrocytes
We extracted human articular chondrocyte mRNA during joint surgey-Using RT-PCR method and Northern blotting method, we demonstrated the expressions of the estrogen, progesterone and androgen receptor gene.
2)Cytokine releases by estradiol in cultured human articular chondrocytes
We added estadiol to the culture medium of cultured human articular chondrocytes and examined releases of IL-1, IL-4, IL6, TNFalpha and TGFbeta. We demonstrated estradiol increases IL-6 and TGFbeta releases.
|
