| Project/Area Number |
07807163
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
小児外科
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| Research Institution | Kyorin University |
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Principal Investigator |
NIRASAWA Yuji Kyorin University, School of Medicine, Associate Professor, 医学部, 助教授 (60129591)
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| Co-Investigator(Kenkyū-buntansha) |
AKAGAWA Kimio Kyorin University, School of Medicine, Professor, 医学部, 教授 (80129303)
ITO Yasuo Kyorin University, School of Medicine, Professor, 医学部, 教授 (80110881)
田中 裕之 杏林大学, 医学部, 助手 (90271270)
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| Project Period (FY) |
1995 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1995: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | HPC-1 antigen / Hirschsprung's disease / exocytosis-related protein / 無神経節部腸管 |
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| Research Abstract |
The HPC-1 antigen was originally identified as a neuron-specific membrane protein in the central nervous system. So, as the first step of the experiments, the presence of HPC-1 antigen in the nervous system of the rat gastrointestinal tract was immunohistochemically confirmed. The HPC-1 is thought to suppress the excess axon-collateral sprouting.
Abnormal extrinsic nerve fibers in Hirschsprung's disease was then immunohistochemically examined using antibody againt the exocytosis-related protein which the HPC-1belongs to. All six exocytosis-related Proteins were succesfully demonstrated in normal sigmoid colon. On the other hand, the HPC-1 was not detected in aganglionic portion of the colon, which may explain the reason of exess extrins nerve fibers in aganglionic portion of the colon in Hirschsprung's disease.
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