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A STUDY OF GENETHERAPY AGAINST ORAL CANCER TARGETTED TO THE GENE OF AUTOCRINE GROWTH FACTOR

Research Project

Project/Area Number 07807184
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

OKAMOTO Tetsuji  HIROSIMA UNIV.SCHOOL OF DENT.PROFESSOR, 歯学部, 教授 (00169153)

Co-Investigator(Kenkyū-buntansha) 高田 和彰  広島大学, 歯学部, 教授 (30029970)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsFGF-1 / FGF-2 / FGF-7 / KGF / serum-free culture / squawous cell cariuown / salivary gland tuwor / avti seuse oligo / dominant-negative reoptor / 自己増殖因子
Research Abstract

Fibroblast growth factor-1 (FGF-1) and FGF-2 are broad spectrum mitogens. Here, th expression of FGF-1, FGF-2 and their receptor, FGF receptor-1 (FGFR-1) was examined i malignant salivary gland tumors and normal salivary glands using immunohistochemical methods. I seven cases of adenoid cystic carcinoma (ACC), both duct-like cells and modified myoepithelial cell were apparently immunopositive for FGF-1, FGF-2 and FGFR-1. In five cases of mucoepidermoi carcinoma (MC), all three types of tumor cells including epidermoid cells, mucous cells an intermediate cells expressed immunoreactive FGF-1, FGF-2 and FGFR-1. In these malignant salivar gland tumors, increased expression of FGFR-1 correlated with the intensity of both FGF- and FGF-2 mmunoreactivity. In contrast to malignant salivary gland tumors, eight cases of normal salivary glan howed negative immunostaining for FGF-1, FGF-2 and FGFR-1 while four cases were wea immunoreactivity for FGF and its receptor.
These results demonstrated that malignant salivary gland tumors overexpress FGF-1, FGF-2 an GFR-1 compared to normal salivary glands, suggesting that these growth factors may play a mportant role in facilitating its neoplastic progression in human salivary gland.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Myoken,Y: "Effect of FGF-1 on the three dimensional grouth and morphogenesis of humansalivary gland enithelial cells in collasen gels" In Vitro Cell.Dev.Biol.31. 84-86 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken,Y: "Immunohistochemical localization of FGF-1 and FGF-2 in cultured human umeloblastoma eplthelial cells" J.Oral Pathol.Med.24. 387-392 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto,T: "Differential expression of FGFR1-FGFR4 genes is associcuted with maligncont progression in huncosr salivary gland tumor" Oral Oncology. 2. 316-319 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto,T: "Expression of VEGF receptor in normal and tumor cells." Oral Oncology. 2. 320-324 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto,T: "Adoptive un mcenotherapy of oral cancer with LAR cells induced in neusly developed seum free medium." Dentistry in Japan. 32. 67-70 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto,T: "Exparession of FGF biuding proxein HBp 17 in nouncll and tumor cells" In Vitro Cell.Dev.Biol.32. 69-71 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken,Y: "Immunohistochemical study of overexpression of FGF-1 FGF-2 and FGF receptor-1 in human malignorit Salivary fumor" J.Pathology. 178. 429-436 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken,Y: "Expression of FGF-1 and FGF-2 and FGFR-1 in a human salivary gland adenocarcinonra cell line" Int.J.Cancer. 65. 650-657 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto,T: "Effects of Insulin and transfeurin on the generation of LAK cells in sesuni free medium." J.Immunological Methods. 195. 7-14 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto,T: "Androgen-dependent expression of FGF-1 in sabmcixillory glancl of mouse" Biochem.Biophys.Res.Comin.221. 795-802 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken,Y: "Immunohistichemical localization of FGF-1,FGF-2 and FGFR 1 inpleomorphic edenoma of the palivary gland." J.Oral Pathol.Mod.26. 17-22 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto,T: "Growth and differentiation of periodontal ligamentderived cells in serum-free defined cultunl." In.Vitro Cell.Dev.Biol. (印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken, Y., Myoken, Y., Okamoto, T., Sato., J.D.and Takada, K.: "Effect of fibroblast growth factor-1 on the three dimensional growth and morphogenesis of human salivary gland epithelial cells embedded in collagen gels." In Vitro Cell.Dev.Biol.31. 84-86 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken, Y., Myoken, Y., Okamoto, T., Sato., J.D.and Takada, K.: "Immunohistochemical localization of fibroblast growth factor-1 (FGF-1) and FGF-2 in cultured human ameloblastoma epithelial cells and ameloblastoma tissues." J.Oral Pathol.Med.24. 387-392 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T., Tanaka, Y., Sakamoto, A.and Takada, K.: "Differential expression of fibroblast growth factor receptor (FGFR) -1-FGFR4 genes is associated with malignant progression in human salivary gland tumor." Oral Oncology. 2. 316-319 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T., Sakamoto, A., Tanaka, Y.and Takada, K.: "Expression of vascular endothelial cell growth factor receptor in normal and tumor cells." Oral Oncology. 2. 320-324 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T., Tani, R., Sakamoto, A., Yabumoto, M., Osaki, T., Tanaka, Y., Toratani, S., Sato, J.D.and Takada, K.: "Adoptive immunotherapy of oral cancer with lymphokine-activated killer (LAK) cells induced in newly developed serum-free medium." Dentistry in Japan. 32. 67-70 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T., Tanaka, Y., Kan, M., Sakamoto, A., Takada, K.and Sato, J.D.: "Expression of fibroblast growth factor binding protein HBp17 in normal and tumor cells." In Vitro Cell.Dev.Biol.32. 69-71 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken, Y., Myoken, Y., Okamoto, T., Sato, J.D., Kan, M., McKeehan, W.L.and Takada, K.: "Expression fo fibroblast growth factor-1 (FGF-1), and FGF-2 and FGF receptor-1 in a human salivary-gland adenocarcinoma cell line : Evidence of autocrine growth." Int.J.Cancer. 65. 650-657 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken, Y., Myoken, Y., Okamoto, T., Sato, J.D., Kan, M., McKeehan, W,L., Nakahara, M.and Takada, K.: "Immunohistochemical study of overexpression of fibroblast growth factor-1 (FGF-1), FGF-2 and FGF receptor-1 in human malignant salivary gland tumors." J.Pathology. 178. 429-436 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T., Myoken, Y., Yabumoto, M., Osaki, T., Fujita, Y., Whitney, R.G., Kan, M., Crabb, J.W., Sato, G.H., Kato, Y., Takada, K.and Sato, J.D.: "Androgen-dependent expression of fibroblast growth factor-1 in submaxillary gland of mouse." Biochem.Biophys.Res.Comm.221. 795-802 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T., Tani, Y., Sakamoto, A., Yabumoto, M., Takada, K., Sato, G.H.and Sato, J.D.: "Effects of insulin and transferrin on the generation of lymphokine-activated killer cells in serum-free medium." J.Immunological Methods. 195. 7-14 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T., Yatsuzuka, N., Tanaka, Y., Kan, M., Yamanaka, T., Sakamoto, A., Takata, T., Akagawa, Y., Sato, G.H., Sato, J.D.and Takada, K.: "Growth and differentiation of human periodontal ligament-derived cells in serum-free defined culture." In Vitro Cell.Dev.Biol.32(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Myoken, Y., Myoken, Y., Okamoto, T., et al.: "Immunohistochemical localization of fibroblast growth factor-1 (FGF-1), FGF-2 and FGF receptor (FGFR-1) in pleomorphic adenoma of the salivary gland." J.Oral Pathol.Med. 26. 17-22 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hashimoto, K., Noshiro, M., Ohno, S., Okamoto, T., et al.: "Characterization of a cartilage-derived 66-kDa protein (RGD-CAP/big-h3) that binds to collagen" Biochemica et Biophysica Acta. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Okamoto, T: "Expression of FGF binding protein HBp/7 in normal and tumor cells" In Vitro Cell. Dev. Biol.32. 69-71 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Myoken, Y: "Immunohistochemical study, of overexpression of FGF-1, FGF-2 and FGF receptor-1 in human malignant salivary tumor" J. Pathology. 178. 429-436 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Myoken, Y: "Expression of FGF-1, FGF-2 and FGF-1 in a human salivary gland adenocarcinoma cell line" Int. J. Cancer. 65. 650-657 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okamoto, T: "Effects of insutin and transferrin on the generation of LAK cells in serum-free medium" J. Immunological Methods. 195. 7-14 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okamoto, T: "Androgen-dependent expression of FGF-1 in submaxillary gland of movse" Biochem. Biophys. Res. Comm. 221. 795-802 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Myoken, Y: "Immunohistochemical localization of FGF-1, FGF-2 and FGFR-1 inpleomaphic adenoma of the salivary" J. Oral Pathol. Med.26. 17-22 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okamoto, T: "Growth and differentiation of periodental ligament-derived cell, in sereem-free defined cultwe" In. Vitro Cell. Dev. Biol. (印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] T. OKAMOTO et al.: "Expression of Vascular Endothelial cell grouth bactor Receptor in normal and tumor cells" Oral Oncology. 2. 320-324 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. OKAMOTO et al.: "Differential Expression of FGF receptor-1-R4 gene is associated with malignant progressim in human salivary" Oral Oncology. 2. 316-319 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. OKAMOTO et al.: "Expression of FGF binding protein HBp17 in normal and tumor cells" In Vitro Cellular Developmental Biology. 32. 69-71 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Myoken et al: "Dverexpression of FGF-2 and FGFR-1 in human malignant Salivary gland tumors." J. Pathology. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Myoken et al: "Expression of FGF-1, FGF-2 and FGF receptor in a Ruman salivary gland adenocarcinonra cell line" Int. J. Cancer. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. OKAMOTO et al: "Androgen-dependent expression of FGF-1 in aubmaxillary gland of mouse," Biochem. Biophys. Res, Comm.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 岡本哲治,明見能成: "細胞成長因子 partIII,Vascular endothelial growth factor" 朝倉書店、日本組織培養学会編(印刷中), (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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