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Solution X-ray scattering study on protein structure at high pressure using synchrotron

Research Project

Project/Area Number 07808076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionRitsumeikan University

Principal Investigator

KATO Minoru  Ritsumeikan Univ., Dept.of Chemistry, Assistant Prof., 理工学部, 専任講師 (00241258)

Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordssolutin X-ray scattering / SOXS / protein structure / pressure / synchrotron radiation / radius of gyration / lysozyme / myoglobin / X線溶液錯乱 / 蛋白質 / 圧力効果
Research Abstract

The purposes of this study are to develop the high-pressure solution X-ray scattering instrument and to apply the technique to the protein solution system. In the development work, we have composed the high-perfomance high-pressure instrument (max.500 MPa) by improving the design of the backup ring and the mechanics of pressure generation. In the application work, we have measured the solution X-ray scattering (SOXS) from lysozyme and myoglobin under pressure. For the estimation of compressibility of protein, we determined the radius of gyration (Rg) of lysozyme under pressure, up to which the protein keeps the native structure. The Rg of lysozyme decreases with increasing pressure : 14.85 * at 1 atm, 14.46 * at 300 MPa. It means that the change in Rg is -0.13 */100 MPa. This value in the absolute is remarkably larger than -0.04 */100 MPa. To clarify the characteristic of pressure unfolding of protein, we measured SOXS from myoglobin at PH 4.4 under pressure up to 30 MPa. The pressure dependence of Rg showed the sigmoid curve reaching to the maximum at 300 MPa. It indicated that the midpoint pressure is about 200MPa, and that the protein prefectly denatured at 300 MPa. The values of Rg at 1atm and 300 MPa are 17.5 * and 21.5 *, respectively. The value at 300 MPa is remarkably smaller than the values pound 30 * reported for the denaturant unfolding of myoglobin. The value for pressure unfolding is rather close to that for molten globule state.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Kato, M., Makino R., and Iizuka, T.: "Thermodynamic Aspects of the CO-Binding Reaction to Cytochromes P450cam. Relevance with Their Biological Significance and Structure" Biochim. Biophys. Acta. 1246. 178-184 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, N., Kato, M., and Taniguchi, Y.: "Pressure- and thermally-Induced Reversivible Canges in the Secondary Structure of Ribonuclease A Studied by FT-IR Spectroscopy" Biochemistry. 34. 5980-5987 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, N., Kato, M., and Taniguchi, Y.: "Pressure-Induced Secondary Structure Change of Ribonuclease A and Ribouclease S Studies by FTIR Spectroscopy" Biospectroscopy. 1. 207-216 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M., and Taniguchi, Y.: "A Hydrostatic Optical cell with Synthetic Diamond Windows for Quantitative Infrared Measurements of Fluids" Rev. Sci. Instrum.66. 4333-4335 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M., Fujisawa, T., Inoko, Y, and Kobayashi, K.: "Small-Angle X-ray Scattering Studies of the Solution Structure of Proteins Under Pressure" Photon Factory Activity Reprot. ♯12. 213 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M. and Fujisawa, T.: "Effect of Pressure on the Solution Structure of Globular Proteins by Small-Angle X-Ray Scattering" Photon Factory Activity Reprot. ♯13. 1991 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 加藤稔,藤沢哲郎: "高圧力下のタンパク質のX線溶液散乱" Photon Factory News, 4 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M., Makino R., and Iizula, T.: "Thermodynamic Aspects of the CO-Binding Reaction to Cytochromes P450cam. Relevance with Their Biological Significance and Structure." Biochim.Biophys.Acta.1246. 178-184 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, N., Kato, M., and Taniguchi, Y.: "Pressure-and thermally-Induced Reversivible Canges in the Secondary Structure of Ribonuclease A Studied by FT-IR Spectroscopy" Biochemistry. 34. 5980-5987 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takeda, N., Kato, M., and Taniguchi, Y.: "Pressure-Induced Secondary Structure Change of Ribonuclease A and Ribouclease S Studies by FTIR Spectroscopy" Biospectroscopy. 1. 207-216 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M., and Taniguchi, Y.: "A Hydrostatic Optical cell with Synthetic Diamond Windows for Quantitative Infrared Measurements of Fluids" Rev.Sci.Instrum.66. 4333-4335 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M., Fujisawa, T., Inoko, Y,and Kobayashi, K.: "Small-Angle X-ray Scattering Studies of the Solution Structure of Proteins Under Pressure" Photon Factory Activity Reprot. #12. 213 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M.and Fujisawa, T.: "Effect of Pressure on the Solution Structure of Globular Proteins by Small-Angle X-Ray Scattering" Photon Factory Activity Report. #13. 1991 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato, M.and Fujisawa, T.: "Solution X-ray scattering from the protein solution at high pressure" Photon Factory News. 13. 12-15 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kato,M.,Makino R.,and Iizuka,T.: "Thermodynamic Aspects of the CO-Binding Reaction to Cytochromes P450cam. Relevance with Their Biological Significance and Structure" Biochem. Biophys. Acta. 1246. 178-184 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeda,N.,Kato,M.and Taniguchi,Y.: "Pressure- and thermally-Induced Reversivible Canges in the Secondary Structure of Ribonuclease A Studied by FT-IR Spectroscopy" Biochemistry. 34. 5980-5987 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takeda,N.,Kato,M.,and Taniguchi,Y: "Pressure-Induced Secondary Structure Changes of Ribonuclease A and Ribouclease S Studies By FTIR Spectroscopy" Biospectroscopy. 1. 207-216 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kato,M.and Taniguchi,Y.: "A Hydrostatic Optical cell with Synthetic Diamond Windows for Quantitative Infrared Measurements of Fluids" Rev. Sci. Instrum.66. 4333-4335 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kato,M.,Fujisawa,T.,Inoko Y.and Kobayashi,K.: "Small-Angle X-ray Scattering Studies of the Solution Structure of Proteins Under Pressure" Photon Factory Activity Reprot. ♯12. 213- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kato,M.and Fujisawa,T.: "Effect of Pressure on the Solution Structure of Globular Proteins by Small-Angle X-Ray Scattering" Photon Factory Activity Reprot. (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] 加藤稔,藤沢哲郎: "高圧力下のタンパク質のX線溶液散乱" Photon Factory News, 4 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Minoru Kato: "A hydrostatic optical cell with synthetic diamond windows for quantitative intrared, measurements of fluids" Rev. Sci. Instrum. 66. 4333-4335 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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