| Project/Area Number |
07808085
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Osaka University |
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Principal Investigator |
NOMURA Midori Osaka University, Res.Inst.microbial diseases, Medical Genetics, Reserch Associate, 微生物病研究所, 助手 (60263315)
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| Co-Investigator(Kenkyū-buntansha) |
TOMOTSUNE Daihachiro Osaka University, Res.Inst.microbial diseases, Medical Genetics, Reserch Associa, 微生物病研究所, 助手 (80283802)
TAKIHARA Yoshihiro Osaka University, Res.Inst.microbial diseases, Medical Genetics, Associate Profe, 微生物病研究所, 助教授 (60226967)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | F9 cells / cell surface protein / glycoprotein / MHC class I / GPI-anchor / gene family / RA-inducible genes / early mouse embryo / Zoo blot / Whole-mount in situ hybridization |
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| Research Abstract |
Rae-1 cDNA is one of the retinoic acid (RA) -inducible cDNA clones in mouse embryonal carcinoma F9 cells. Rae-1 mRNAs were not detected in adult mouse tissues, but were detected in early mouse embryos, sepecially in the frontal part of the 10-day-embryo brain.
The primary structure of RAE-1 protein has a weak, but significant homology with major histocompatibility complex (MHC) class I molecules. We confirmed that RAE-1 is a GPI-anchored and cell surface glycoprotein, using phosphatidyl inositol-spesific phospholipase C (PI-PLC) and inhibitor of glycosylation, tunicamycin, respectively. Furthermore, at least three different kinds of Rae-1 cDNAs (alpha, beta and gamma) were identified and the overall nucleotide sequence homology among these cDNAs was about 98%. Then, we isolated three kinds of the corresponding genes and mapped these genes on mouse chromosome 10A4 region by FISH.Interestingly, expression patterns were different between rae1-alpha and rae1-beta, gamma genes, which was considered to be correlated with the structural features that 5'-upstream region of rae1-alpha gene was different from that of rae1-beta, gamma genes.
The rae-1 genes encode early-embryo specific, GPI-anchored cell surface glyoproteins. They constitute a novel gene family which may be involved in cell-cell interactions during early mammalian development.
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