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Analysis of the rate of the production and the function of organic substances in dinoflagellate symbiotic algae

Research Project

Project/Area Number 07836005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section時限
Research Field 海洋生物学
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

SHIRAIWA Yoshihiro  Niigata University Faculty of Science Associate Professor, 理学部, 助教授 (40126420)

Co-Investigator(Kenkyū-buntansha) ODANI Shoji  Niigata University Faculty of Science Professor, 理学部, 教授 (60018702)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1995: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsCalcification / CO_2 fixation / Foraminifera / Photosynthetic activity / Symbiodinium / Symbiotic algae isolated / 生育至適条件 / 光補償点 / CO_2親和性 / 酸素阻害 / 光合成特性 / 石灰化 / サンゴ
Research Abstract

Symbiotic algae have a very important role for the production of organic substances in invertebrates living in coral reef. Isolated symbiotic algae (Symbiodinium sp.) from Amphiscolops sp., Corculum cardisa, Pocilopora damicornis were used for the estimation of the photosynthetic ability. (1) An artificial seawater (Marine Art, Senju Pharmaceutical Co.Ltd.) enriched with a modified Erd-Schreiber's medium was useful for getting high growth rate. (2) Optimum temperature for the growth was 30゚C and no algal growth was observed blow 5゚C.(3) Photosyntheis saturated at 150 mumol/m^2/s and the light compensation point was 10 mumol/m^2/s. (4) Optimum pH was 6-8. (5) Determinations of the activities of photosynthesis and respiration, and change in pH of the medium in Foraminifera, Scleractinian coral Anthopora, and a green macrophyte Halimeda, which were performed in Palau, suggested that Foraminifera is possible to play an important role for the fixation and storage of CO_2. (6) Calcifying photosynthetic organisms are possible to be great CO_2 sink when those photosynthesis are fully active. (7) A new method for the separation and analyzes of strongly hydrophilic peptides using HPLC was developed for the analyzes of the metabolisms in symbiotic algal cells and host cells.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Shiraiwa, Y.: "Diurnal change in the rate of the utilization of CO_2 by Foraminifera (Protozoa) and a hermatypic coral, Scleractinia (Coelenterata)." The Sohgen Maru in Palau and Pohnpei 1995.108-115 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekino, K.: "CO_2 release during photosynthesis and respiration from the culture of calcifying and non-calcifying marine unicellular algae." Jounal of Marine Biothechnology. 3. 101-104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekino, K.: "Role of coccoliths on the utilization of inorganic carbon by a marine unicellular coccolithophorid, Emiliania huxleyi : a survey using intact cells and protoplasts." Plant Cell Physiology. 37. 123-127 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekino, K.: "Evidence for the involvement of mitochondrial respiration in calcification in a marine coccolithophorid, Emiliania huxleyi." Plant Cell Physiology. 37. 1030-1033 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoh, A.: "Two low-CO_2 inducible polypeptides in soluble protein fractions in Chlorella regularis grown at acidic or alkaline pHs." Plant Cell Physiology. 37. 431-437 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K. Baba: "Separation of strongly hydrophilic peptides on the reversed phase HPLC by reversible chemical modifications." Chromatography. 17. 183-188 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shiraiwa, Y.: "Diurnal change in the rate of the utilization of CO_2 by Foraminifera (Protozoa) and a hermatypic coral, Scleractinia (Coelenterata)" The Sohgen Maru in Palau and Pohnpei 1995, PP.108-115, Edited by M.Endo.Marine Biotechnology Institute (Tokyo, Japan). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekino, K.and Shiraiwa, Y.: "CO_2 release during photosynthesis and respiration from the culture of calcifying and non-calcifying marine unicellular algae." J.Marine Biotech. 3. 101-104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekino, K., Kobayashi, H.and Shiraiwa, Y.: "Role of coccoliths on the utilization of inorganic carbon by a marineunicellular coccolithophorid, Emiliania huxleyi : -a survey using intact cells and protoplasts." Plant Cell Physiol. 37. 123-127 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sekino, K.and Shiraiwa, Y.: "Evidence for the involvement of mitochondrial respiration in calcification in a marine coccolithophorid, Emiliania huxleyi." Plant Cell Physiol. 37. 1030-1033 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoh, A.and Shiraiwa, Y.: "Two low-CO_2 inducible polypeptides in soluble protein fractions in Chlorella regularis grown at acidic or alkaline pHs." Plant Cell Physiol. 37. 431-437 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] K.Baba, T.Yoshida, T.Abe, and S.Odani: "Separation of strongly hydrophilic peptides on the reversed phase HPLC by reversible chemical modifications." Chromatography. 17(3). 183-188 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shiraiwa,Y.: "Diurnal change in the rate of the utilization of CO_2 by Foraminifera (Protozoa) and a hermatypic coral,Scleractinia (Coelenterata)." The Sohgen Maru in Palau and Pohnpei 1995.108-115 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sekino,K.: "CO_2 release during photosynthesis and respiration from the culture of calcifying and non-calcifying marine unicellular algae." Journal of Marine Biotechnology. 3. 101-104 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sekino,K.intact: "Role of coccoliths on the utilization of inorganic carbon by a marine unicellular coccolithophorid,Emiliania huxleyi : a survey using intact cells and protoplasts." Plant Cell Physiology. 37. 123-127 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sekino,K.: "Evidence for the involvement of mitochondrial respiration in calcification in a marine coccolithophorid,Emiliania huxleyi." Plant Cell Physiology. 37. 1030-1033 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Satoh,A.: "Two low-CO_2 inducible polypeptides in soluble protein fractions in Chlorella regularis grown at acidic or alkaline pHs." Plant Cell Physiology. 37. 431-437 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K,Baba: "Separation of strongly hydrophilic peptides on the reversed phase HPLC by reversible chemical modifications." Chromatography. 17. 183-188 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ramazanov,Z.: "Effect of external CO_2 concentrations on protein synthesis in the green algae Scenedesmusobliquus(Turp.)Kutz and Chlorella vulgaris (Kosikov)." Planta. 197. 272-277 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sekino,K.: "CO_2 release during photosynthesis and respiration from the culture of calcifying and non-calcifying marine unicellular algae." J.Marine Biotechnology. 3. 101-104 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Sekino,K.: "Role of coccoliths on the utilization of inorganic carbon by a marine unicellular coccolithophorid,Emiliania huxleyi:a survey using intact cells and protoplasts." Plant Cell Physiol.37(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Satoh,A.: "Twolow-CO_2 inducible polypeptides in soluble protein fractions in Chlorella regularis grown at acidic or alkaline pHs." Plant Cell Physiol.37(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 沢田健: "堆積物中の調査アルケノン,アルケノエイトによる円石藻群集組成の復元の試み:培養実験からのアプローチ." Research of Organic Geochemistry. 10. 21-26 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishizuka,T.: "Syanaphin:A protein associated with the docking/fusion complexes in presynaptic terminals." Biochem.Biophys.Res.Commun.213. 1107-1114 (1995)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-21  

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