| Project/Area Number |
07838035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 時限 |
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| Research Field |
咀嚼
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| Research Institution | Showa University |
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Principal Investigator |
HAMBA Michiko Dental School of Showa University, Associated Professor, 歯学部, 講師 (40119251)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Hiroyuki Dental School of Showa University, Assistant, 歯学部, 助手 (00224220)
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| Project Period (FY) |
1995 – 1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1996: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1995: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | proto-oncogen / c-tos-immunoreactive / Interleukin-IB / trigeminal caudalis / sustained pain / orthodontic stimulation / periodontal disease / Glutamate-receptor / Proto-Oncogene / Interleukin-1B / Interleukin / NSAIDs |
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| Research Abstract |
1 The expression of c-fos-like immunoreactivity (For-IR) was studied in the trigeminal caudalis of the chloralose anesthetized Wister rats after injection of 0.05 ug of interleukin-1-bata (IL-1b) into the gingiva of the incisor or application of the orthodontical mechanical stimulation. IL-1b injection-induced Fos-IR (IL-1b) was suppressed by administration of non-steroidal anti-inflammatory drugs (NSAIds) at the early period postinjection. The results suggest that a small amount of IL-1b at the site of periodontal disease can induce Fos-IR in pain-processing neurons.
2 The effects of the antagonists and agonists of glutamate receptors on the expression of Fos-IR were examined after tooth pulp stimulation. MK-801, CNQX,AP-3, NMDA,AMPA and phorbol ester were intrathecally administered to rats 10 min prior to stimulation (0.2 ms, 0.6 mA,0.5 Hz, 10 min) ; the effects or each agent on the expression of Fos-IR was studied in the caudalis at 2 h post-stimulation. The results suggest that Ca^<2+> entry through an NMDA receptor is crucial to the induction of c-Fos and to the development of persistent pain.
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