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Mechanoreceptors in Alveolar Osteocyte

Research Project

Project/Area Number 07838043
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section時限
Research Field 咀嚼
Research InstitutionKanagawa Dental College

Principal Investigator

TAKAGAKI Yuko  Kanagawa Dental College, OralBiochemistry, Lecturer, 歯学部, 講師 (60050689)

Co-Investigator(Kenkyū-buntansha) SAITO Shigeru  Kanagawa Dental College, OralBiochemistry, Professor, 歯学部, 教授 (80084713)
Project Period (FY) 1995 – 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsosteocyte / mechanical stress / alveolar bone / stretchig / proliferation / development / bone formation / 骨形成
Research Abstract

Although it is well known that bone formation is accelerated by mechanical stimuli, the signal transduction pathways involved are largely unknown. Suggested by the studies by E.Burger and others, which showed that bone cells respond to high-level and low-level stresses differently, we compared the response of newborn rat calvarial cells to mechanical stress during their development from immature osteoblasts to mature osteoblasts, further into osteocytes. We showed that the type of cells which is most sensitive to physiological stretching of less than 4,0000 mustrain is not osteoblast but young osteocyte. These cells are in transition from osteoblasts to osteocytes expressing low-level alkaline phosphatase and high-level osteocalcin. They also develop extensive cell processes which form a network of cells in bone. These young osteocytes stop proliferation in response to stretching and mineralize thir matrix. It was shown that cAMP is secreted and the expression of IGF-I and osteocalcin is induced in response to the stress.
In human alveolar bone, it was not easy to clean the bone chips prior to the isolation of cells. That is probably why we could not obtain as many differentiated osteocytes as in rat calvaria. In those few successful cases, however, the response of osteocytes to the above mentioned stretching was similar to that of rat osteocytes. In other words, it was shown by RT-PCR method that osteocalcin expression was upregulated as a result of stretching. It was also suggested that amiloride sensitive channel (s) is involved in their response to stretching from our results of channel blocker studies measuringCa influx upon the exposure to lower osmotic conditions.

Report

(3 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • 1995 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Satoyoshi,M.et,al.: "Extracellular processing of dentin matrix protein in mineralizing odontoblast culture." Calcif. Tissue Int.57. 237-241 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikuni-Takagaki,Y.et,al.: "Matrix mineralization and the differentiation of osteocyte-like dells in culture." J.Bone Miner.Res.10. 231-242 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikuni-Takagaki,Y.at.al.: "Extracellular processing of dentin matrix protein in mineralizing odontoblast culture." Endocrinology. 137. 2028-2035 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 小泉忠彦,高垣裕子,寺中敏夫: "培養ウシ象牙芽細胞のビタミンD_3に対する応答" 日本歯科保存学雑誌. 39. 865-873 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki,Y.et.al.: "Extracellular processing of bone and dentin proteins in matrix mineralization" Connect. Tissue Res. 35. 277-283 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 高垣裕子: "骨形成系細胞の機械的外力に対する応答" 日本骨形態計測学会誌. 7(印刷中). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoyoshi, M., Koizumi, Teranaka, T., Iwamoto, T., Takita, H., Kuboki, Y.Saito, S., Mikuni-Takagaki, Y.: "Extracellular processing of dentin matrix protein in mineralizing odonto-blast culture." Calcif.Tissue Int.57. 237-241 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikuni-Takagaki, Y., Kakai, Y., Satoyoshi, M., Kawano, E., Suzuki, Y., Kawase, T., Saito, S.: "Matrix mineralization and the differentiation of osteocyte-like cells in culture" J.Bone Miner.Res.10. 231-242 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takagaki, Y.: "The 17^<th> ASBMR Meeting Report 8.Osteocyte" JSBMR. 13. 200-202 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikuni-Takagaki, Y., Suzuki, Y., Kawase, T., Saito, S.: "Distinct responses of different populations of bone cells to mechanical stress" Endocrinology. 137. 2028-2035 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Koizumi, T.: "Takagaki, Y., Teranaka, T. : Response of cultured bovine odontoblast to vit.D_3" Jap.J.Resto.Dent.39. 865-873 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suzuki, Y., Kubota, T., Koizumi, T., Satoyoshi, M., Kawase, T., Saito, S.: "Mikuni-Takagaki, Y., Extracellular processing of bone and dentin proteins in matrix mineralization" Connect.Tissur Res.35. 277-283 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takagaki, Y.: "The 18^<th> ASBMR Meeting Report.11.Osteocyte." JSBMR. 14. 162-163 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takagaki, Y.: "Response of bone forming cells to mechanical stress" Jap.J.Bone Morphometry. 7 (No.1)(in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoyoshi,M.et.al.: "Extracellular processing of dentin matrix protein in mineralizing odontoblast culture." Calcif.Tissue Int.57. 237-241 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mikuni-Takagaki,Y.et.al.: "Matrix mineralization and the differentiation of osteocyte-like cells in culture." J.Bone Miner.Res.10. 231-242 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mikuni-Takagaki,Y.et.al.: "Extracellular processing of dentin matrix protein in mineralizing odontoblast culture." Endocrinology. 137. 2028-2035 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 小泉忠彦,高垣裕子,寺中敏夫: "培養ウシ象牙芽細胞のビタミンD_3に対する応答" 日本歯科保存学雑誌. 39. 865-873 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suzuki,Y.et.al.: "Extracellular processing of bone and dentin proteins in matrix mineralization" Connect.Tissue Res.35. 277-283 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 高垣裕子: "骨形成系細胞の機械的外力に対する応答" 日本骨形態計測学会誌. 7(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Satoyoshi, M. et. al.: "Extracellular processing of dentin matrix protein in mineralizing odontoblast culture." Calcif. Tissuc Int.57. 237-241 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mikuni-Takagaki, Y.: "Matrix mincralization and the differentiation of osteocyte-like cells in culture." J. Bone Miner. Res.10. 231-242 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Mikuni-Takagaki, Y.: "Extracellular processing of dentin matrix protein in mineralizing odontoblast culture." Endocrinology. 137. 2028-2035 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小泉忠彦,高垣裕子,寺中敏夫: "培養ウシ象牙芽細胞のビタミンD_3に対する応答" 日本歯科保存学雑誌. 39(印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki, Y. et. al.: "Extracellular processing of bone and dentin proteins in matrix mineralization" Connect. Tissue Res.34(印刷中). (1996)

    • Related Report
      1995 Annual Research Report

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Published: 1995-04-01   Modified: 2016-04-25  

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