| Project/Area Number |
08041177
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Field Research |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Kochi Medical School |
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Principal Investigator |
HASHIGUCHI Yoshihisa Kochi Medical School, Parasitology, Professor, 医学部, 教授 (10037385)
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| Co-Investigator(Kenkyū-buntansha) |
MARUNO Motomi University of the Ryukyus, Dermatology, Research Associate, 医学部, 助手 (00264507)
MIMORI Tatsuyuki Kumamoto Univ.Sch.Med., Tumor Genetics and Biology, Assoc.Prof., 医学部, 助教授 (00117384)
HOSOKAWA Atsushi University of the Ryukyus, Dermatology, Assistance Professor, 医学部, 講師 (10181497)
FURUYA Masato Kochi Medical School, Laboratory Animals, Associate Professor, 医学部, 助教授 (00035437)
NONAKA Shingo University of the Ryukyus, Dermatology, Professor, 医学部, 教授 (10039571)
フラード M.H. グアヤキル大学, 医学部, 教授
ルンベア G. J. グアヤキル大学, 医学部, 教授
ラッソーS. R.F. グアヤキル大学, 医学部, 教授
E.A. ゴメス カトリカ大学, 医学部, 助手
松本 芳嗣 東京大学, 農学部, 助教授 (00173922)
是永 正敬 高地医科大学, 医学部, 助手 (00128274)
片倉 賢 慈恵会医科大学, 医学部, 助手 (10130155)
原田 正和 香川医科大学, 医学部, 助手 (90127580)
村主 節雄 香川医科大学, 医学部, 助教授 (00032897)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 1997: ¥7,700,000 (Direct Cost: ¥7,700,000)
Fiscal Year 1996: ¥7,600,000 (Direct Cost: ¥7,600,000)
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| Keywords | Ecuador / Cutaneous leishmaniasis / Mephaquin / Artesunate / Treatment / Oral treatment / Skin biopsy / Patients |
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| Research Abstract |
In Ecuador, a leishmaniasis project entitled "Studies on New World leishmaniasis and its transmission, with particular refernce to Ecuador" has been carried out from 1986 to date. During the research term, a considerable information has been accumulated and the result obtained has been used for the public health administration in that country. In the present research period from 1996 to 1997, epidemiological and clinical data were collected, in addition to diagnosis and treatment of patients with cutaneous leishmaniasis. Regarding to Leishmania isolates from patients, vector sandflies and reservoir hosts, they were characterized by using different types of techniques, such as karyotype analysis, ELISA and etc. Based on the results obtained, a trial has been done to depict the geographical distribution of each species of the genus Leishmania in Ecuador. DNA sequencings of the parasites and sandflies were also made using materials collected in different endemic areas of Ecuador for the purpose of their classification and epidemiological appllication. Pathological observations were also made electon-microscopically in animals infected experimentally with Leishmania parasites from CL (cutaneous leishmaniasis) and DCL (diffuse cutaneous leishmaniasis) origin. In order to get more sensitive and specific method for diagnosis of the disease, PCR using different primers was compared with traditionally-used techniques, such as smears, cultures and others.
A clinico-epidemiological survey was performed in several endemic areas located at the Pacific Coastal regions of Ecuador, in addition to differential diagnosis between cutaneous leishmaniasis and other skin diseases.
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