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International collaboration of neuroblastoma

Research Project

Project/Area Number 08042002
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionSpecial Cancer Research
Research Field Pediatrics
Research InstitutionThe University of Tokyo

Principal Investigator

HAYASHI Yasuhide  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 講師 (30238133)

Co-Investigator(Kenkyū-buntansha) NAKAGAWARA Akira  Chiba Cancer Center, Chairman, 生化学, 部長
KOBAYASHI Miyuki  University of Tokyo, Faculty of Medicine, Asistant Professor, 医学部・附属病院, 助手 (60205391)
BESSHO Fumio  University of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 助教授 (40010285)
LOOK Thomas A  聖ユダ小児研究病院, 実験腫瘍, 教授
BRODEUR Garrett M.  Philadelphia Children's Hospital, Director, Professor, 教授
LOOK A. Thomas  St. Jude Children's Research Hospital, Director, Professor
LOOK Thomas  聖ユダ小児研究病院, 実験腫瘍, 教授
BRODEUR Garr  フィラデルフィア小児病院, 教授
HOE Tuck San  マレーシア国民大学, 助教授
THOMAS A.Loo  St. Jude小児研究病院, 教授
GARRETT M.Br  フィラデルフィア小児病院, 教授
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1997: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1996: ¥3,000,000 (Direct Cost: ¥3,000,000)
Keywordsneuroblastoma / N-myc gene / p16 gene / DCC gene / DPC4 gene / MADR2 gene / p73 gene / mass screening / p16遺伝子 / p15遺伝子 / 疫抑制遺伝子 / マススクリーニング / N-myc遺伝子
Research Abstract

N-myc, p16, DCC, DPC4, MADR2 and p73 genes were analyzed in Japan, USA and Malaysia by Southern blotting, Northern blotting, Western blotting and PCR-single stand conformation polymorphism (SSCP). Loss of heterozygosity (LOH) in our Japanese study suggested high frequent LOH between D9S162 and IFNA on 9p, which included p16 gene. Homozygous deletion (HD) of p16 gene was not found in 81 Japanese samples (N-myc 6 samples). PCR-SSCP analysis identified only missense mutation, suggesting polymorphism. Absence or decreased expression of p16 gene was found in 6 of 10 Japanese cell lines and 6 of 9 USA cell lines, suggestings no significant difference between them. Hypermenthylation of the p16 gene was found in 6 of 10 Japanese cell lines and 6 of 9 USA cell lines, suggesting that hypermenthylation plays an important role for the development or progression of neuroblastoma. The commonly deleted region of 18q in neuroblastoma included DCC, DPC4 and MADR2 genes. Absence or reduced expression of DCC gene was found in half of the samples in 3 countries. PCR-SSCP analysis of DCC gene showed only missense mautation, suggesting polymorphism. Alterations of DPC4 and MDRR2 genes were relatively rare. These results suggested that DCC gene plays an important role in the dissemination of neuroblastoma, and that DPC4 and MAD2 gene are not involved in the development of neuroblastoma. Frequency of alterations of the p73 gene was not different among 3 countries. Our epidemiological study showed the frequency of the development of neuroblastoma increased (2 times) after mass screening. These results were found to be different from those in USA and Canada. Inetrnational Collaboration of neuroblastoma contributed to the pathogenesis of neuroblastoma in 3 countries.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Bessho F. et al.: "Effects of mass screening on age-specific incidence ofneuroblastoma"Int. J. Cancer. 67. 520-522 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Bessho F. et al.: "Where should neuroblastoma mass screening go?"Lancet. 348. 1672 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takita J. et al.: "Deletion map of Chromosome 9 and p16 (CDKN2A) gene alterations in neuroblastoma"Cancer Res.. 57. 907-912 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kong X-T et al.: "Expression and mutational analysis of the DCC, DPC4, and MADR2/JV18-1 genes in neuroblastoma"Cancer Res.. 57. 3772-3778 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakagawara A. et al.: "High levels of expression and nuclear localization of interleukin-1 beta converting Enzyme (ICE) and CPP32 in favorable human neuroblastomas"Cancer Res.. 57. 4578-4584 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamamoto K. et al.: "Spontaneous regression of localized neuroblastoma detected by mass screening"J. Clin Oncol. 16. 1265-1269 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Bessho F. et al.: "Effects of mass screening on age-specific incidence of neuroblastoma"Int. J. Cancer. 67. 520-522 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] F. Bessho: "Where should neuroblastoma mass screening go?"Lancet. 348. 1672 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takita J, Hayashi Y, Kohno T, Yamaguchi N, Hanada R, Yamamato K, Yokota J: "Deletion map of Chromosome 9 and p16(CDKN2A) gene alterations in neuroblastoma"Cance Res. 57. 907-912 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kong X-T, Choi Sh, Xu Feng, Chen T, Hayashi Y: "Expression and mutational analysis of the DCC, DPC4 and MADR2/JV18-1 genes in neuroblastoma"Cancer Res. 57. 3772-3778 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nakagawara A, Nakamura Y, Ikeda H, Hiwasa T, Kuida K, Su MS, Zhao H, Cnaan A, Sakiyama S: "High levels of expression and nuclear localization of interleukin-1 beta converting Enzyme (ICE) and CPP32 in favorable human neuroblastomas"Cancer Res. 57. 4578-4584 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yamamoto K, Hanada R, Kikuchi A, Ichikawa M, Aihara T, Oguma E, Moritani T, Shimanuki Y, Tanimura M, Hayashi Y: "Spontaneous regression of localized neuroblastoma detected by mass screening"J Clin Oncol. 16. 1265-1269 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takita J.et al.: "Delection map of chromosome 9 and p16(CDKN2A) gene alterations in neuroblastoma." Cancer Research. 57. 907-912 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kong X-T, et al.: "Consistent detection of TLS/FUS-ERG chimeric transcripts in acute myeloid leukemia with t(16;21)(p11;q22) md identification of a novel transcript." Blood. 89. 1192-1199 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamamoto K.et al.: "Natural history of neuroblastoma found by mass screening." Lancet. 349. 1102 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kong X-T, et al.: "Expression and mutational analysis of the DCC,DPC4,and MADR2/JV18-1 genes in neuroblastoma." Cancer Research. 57. 3772-3778 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Taki T, et al.: "The t(11;16)(q23;p13) translocation in myelodysplastic syndrome fuses the MLL gene to the CBP gene." Blood. 89. 3945-3950 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ida K, et al.: "Adenoviral E1A-associated protein p300 is involved in acute myeloid leukemia with t(11;22)(q23;p13)" Blood. 12. 4699-4704 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomohiko Taki: "Fusion of the MLL gene with two different genes,AF6 and AF-5α,by a complex translocation involving chroinosomes 5,6,8 and 11 in intant leukemia" Oncogene. 13. 2121-2130 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shigetoshi Kobayashi: "Mutations of the Btk gene in twelve unrelated families with X-linked ■ emia in Japan : Immunological phamatypes are increase that with the location of the mutation" Human Genetics. 97. 424-430 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroaki Ohnishi: "Infrequent mutations of the P53 gene in hepatoblastomas" Gene Chromosome Cancer. 15. 187-190 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroaki Ohnishi: "Homozygous deletions of P16/MTS1 and P15/MTS2 genes in t (1;19)-negative but not in t (1;19)-positive B precursor acute lymphoblastic leukemia in childbord" Leukemia. 10. 1104-1110 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tomohiko Taki: "Frequency and clinical significance of the MLL gene rearrangements in infant acute leukemia" Leukemia. 10. 1303-1307 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harumi Kakuda: "A novel human leukaemic cell line,CTS,has a t (6;11) chromosomel translocation and characteristics of pluripotent stem cells." British Journal of Haematology. 95. 306-318 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshiaki Tsuchida: "Genetic clinical narkers of human neuroblastomd with special reference to N-myc oncogene : A litied or not amelited?-Oreview" Tumor Biology. 17. 65-74 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akira Inoue: "Competitive polymerase chain reaction for the quantification of N-myc gene number in neuroblastond" Tumor Biology. 17. 262-270 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Atushi Ohshima: "11q23 aberation is an additional chromosomal change in de novo acute leukemia after treatment with Etoposide and Mitsxantrono." American Journal of Hematology. 53. 264-266 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] D.Yamashiro: "Expression of TRK-C in favorable human neuroblastoma" Oncogene. 12. 37-41 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.A.Reale: "Loss of DCC expression in neuroblastoma is associated with disease dissemination." Clinical Cancer Research. 2. 1097-1102 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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