| Project/Area Number |
08044069
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University |
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Principal Investigator |
YOSHIKAWA Kenichi Graduate School of Human Informatics, Nagoya University, Professor, 大学院・人間情報学研究科, 教授 (80110823)
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| Co-Investigator(Kenkyū-buntansha) |
VASILEVSKAYA V.V. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Le, ネスメヤノB有機素子化合物研究所, 上級研究員
KHOKHLOV A.R. Nesmeyanov Institute of Organoelement Compounds, Russian Academy of Sciences, Pr, ネスメヤノフ有機素子化合物研究所, 教授
KUMAZAWA Noriyuki Faculty of Engineering, Ibaragi University, Assistant professor, 工学部, 助教授 (70137256)
VASILEVSKAYA バレンチナ ヴィ ロシア科学アカデミー, ネスメヤノフ有機素子化合物研究所, 上級研究員
VASILEVSKAYA バレンチナ ヴイ ロシア科学アカデミー, ネスメヤノフ有機素子化合物研究所, 上級研究員
松沢 有希子 豊橋技術科学大学, 工学部, 助手 (10273335)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1997: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Single chain observation / Coil-globule transition / First order phase transition / Fluorescence microscopy / Monte Carlo simulation / Long DNA chain / Collapse of DNA / Folding transition / 単分子凝縮 / モンテカルロシミュレーション / 単分子備品 |
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| Research Abstract |
As a whole, the international collaboration between Nagoya and Moscow has been performed successfully, including the development on basic concept in polymer physics and also in physical chemistry in mesoscopic system. Through the collaboration, the research group of Nagoya has demonstrated that individual single duplex DNA chains undergoes large discrete phase transition between elongated coil and compacted globule (coil-globule transition), based on the single chain observation by use of fluorescence microscopy, whereas ensemble of DNA has the characteristics of diffuse or continuous transition.From single chain observation, we found this first order phase transition is induced by various kinds of condensation agents such as synthetic polymer, cationic surfactant, low molecular multivalent cation, and alcohol. Based on these experimental findings, active collaboration between Nagoya and Moscow has been performed on the theoretical analysis of the phase transition in a single polymer chain, including Monte Carlo simulation and mean field theory. The essential aspects in the collapsed transition of long DNA chain has been reproduced with these theoretical analysis and calculations. It is to be noted that the existence of the morphological variation in the collapsed products can be interpreted well in these theories.
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