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MOLECULAR MECHANISM OF SORTING OUT OF CELLULAR SLIME MOLDS

Research Project

Project/Area Number 08044184
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Developmental biology
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

OCHIAI Hiroshi  HOKKAIDO UNIVERSITY,GRADUATE SCHOOL OF SCIENCE,PROFESSOR, 大学院・理学研究科, 教授 (10002122)

Co-Investigator(Kenkyū-buntansha) FUKUZAWA Masashi  UNIVERSITY OF DUNDEE,GRADUATE SCHOOL OF SCIENCE,PDF, 理学部, 博士研究員
SAITO Tamao  GRADUATE SCHOOL OF SCIENCE,RESEARCH ASSOCIATE, 助手 (30281843)
WILLIAMS Jeffrey  UNIVERSITY OF DUNDEE,GRADUATE SCHOOL OF SCIENCE,PROFESSOR, 理学部, 教授
NELLEN Wolfgang  KASSEL UNIVERSITY,GRADUATE SCHOOL OF SCIENCE,PROFESSOR, 理学部, 教授
BOZZARO Salvatore  TORINO UNIVERSITY,DEPARTMENT OF MEDICINE,PROFESSOR, 医学部, 教授
JEFFREY Will  ダンディ大学, 理学部, 教授
WOLFGANG Nel  カッセル大学, 理学部, 教授
SALVATORE Bo  トリノ大学, 医学部, 教授
WILLIAM Loom  カリフォルニア大学, 生物学部, 教授
舟本 聡  北海道大学, 大学院・理学研究科, 特別研究員
前田 ミネ子  大阪大学, 大学院・理学研究科, 助手 (70029700)
NOEGEL Angel  マックスプランク生化学研究所, 細胞生物, 助教授
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥11,700,000 (Direct Cost: ¥11,700,000)
Fiscal Year 1998: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1997: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1996: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsSORTING OUT / Polysphondylium / Dictyostelium / CELL-CELL ADHESION / GENE DISRUPTION / DOUBLE MUTANTS / DOUBLE KNOCK OUT MUTANTS / Polyspondylium / 分子遺伝学 / 細胞性粘菌 / 分子生物学
Research Abstract

The major results obtained during this project have been described here.
1. At the late development of the cellular slime mold Polysphondylium, there occurs the secondary stalk formation. The possibility to be as a suppressor of the secondary stalk formation was undertaken.
Namely gp64 was forced to express in Dictyostelium cells continuously by the way of the fusion protein directing an ecmB promotor. The secondary formation of stalk was completely stopped, indicating that gp64 inhibits the precocious secondary formation of the stalks.
2. IagC is one of a few critical genes involved in the tight mound formation and its further development of Dictyostelium, and recently a related sequence isolated from ESTs of the Japan cDNA project, designated as lagCII, had been found. To see the functional role of this lagCII, we knocked out this gene, but with no phenotypic change. Further we made a double knock out mutant which both lagC and lagCII genes were recombined by homologously, it was again no change of phenotype. Therefore we are undertaking the analysis of overexpressor of lagCII.
3. To isolate a new cell-cell adhesion protein in the late development of Dictyostelium, we made mutants which lagC and gp8O genes were
doubly knocked out. The mutant pehnotype showed a delayed expression of a EDTA-resistant cell-cell adhesion during late development, indicating that there are another cell adhesion molecule(s) in the late development.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (93 results)

All Other

All Publications (93 results)

  • [Publications] Hiroshi Ochiai: "Dimer formation of a putative cell-cell adhesion protein, gp64 of the cellular slime mold, Polysphondylium pallidum" Plant Cell Physiol. 37・2. 135-139 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoru Funamoto: "Antisense RNA inactivation of gene expression of a cell-cell adhesion protein (gp64) in the cellular slime mold Polysphondylium pallidum." J.Cell Sci.109. 1009-1016 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 舟本 聡: "mRNAとこれに相補的なアンチセンスRNA転写産物の簡便な検出法." 生化学. 68(4). 303-305 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamao Saito: "Identification of a novel all-cis-5, 9, 12-heptadecatrienoic acid in the cellular slime mold Polysphondylium pallidum" Lipids. 31. 445-447 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuyuki Nakata: "Inhibitory effects of tunicamycin on cell-cell adhesion and cell reaggregation of the cellular slime mold, Polysphondylium pallidum" J.Plant Res.109. 193-199 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masashi Fukuzawa: "Molecular cloning and characterization of the cDNA for discoidin II of Dictyostelium discoideum" Plant Cell Physiol. 37. 505-514 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Marco Trinchera: "Dictyostelium cytosolic fucosyltransferase synthesizes H type 1 trisaccharide in vitro" FEBS Letters. 395. 68-72 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Francisco Rivero: "The role of the cortical cytoskeleton : F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development" J.Cell Sci. 109. 2679-2691 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuyuki Yamaguchi: "Targeted disruption of genes for gp138, a cell-fusion-related protein in Dictyostelium discoideum, revealed the existence of a third gene" Develop. Growth Differ.38. 271-279 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Min Pi: "Analysis of a tRNA Gene-Like Sequence (t-Element) with TTA at the Anticodon Position in the Mitochondrial DNA of Dictyostelium discoideum" J.Plant Res.109. 1-6 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Enrico Bracco: "Cloning and transcriptional regulation of the gene encoding the vacuolar/H^+ATPase B subunit of Dictyostelium discoideum" FEBS Letters. 419. 37-40 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuhiro Aiba: "Isoforms of gp138, a Cell-Fusion Related Protein in Dictyostelium discoideum" J.Biochem.121. 238-243 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shinji Ogawa: "Group-l introns in the cytochrome oxidase genes of Dictyostelium discoideum : two related ORFs in one loop of a group-l intron, a cox1/2 hybrid gene and an unusually large cox3 gene" Curr.Genet.31. 80-88 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kayoko Komori: "Cloning and characterization of the gene encoding a mitochondrially localized DNA topoisomerse II in Dictyostelium discoideum" Biochi, Biophys. Acta. 1352. 63-72 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kayoko Komori: "Localization of a DNA Topoisomerase II to Mitochondria in Dictyostelium discoideum : Deletion Mutant Analysis and Mitochondrial Targeting Signal Presequence" J.Plant Res.110. 65-75 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shinji Ogawa: "A site-specific endonuclease specified by one of two ORFs encoded by a group I intron in Dictyostelium discoideum mitochondrial DNA" Gene. 191. 115-121 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yuzuru Kubohara: "Efficient Induction by DIF-1 and 8-bromo Cyclic AMP of Prespore-to-Stalk Conversion in Dictyostelium discoideum" Comp.Biochem. Physiol.118(3). 841-845 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Laurence Aubry: "The Dictyostelium Mitogen-activated Protein Kinase ERK2 Is Regulated by Ras and cAMP-dependent Protein Kinase (PKA) and Mediates PKA Function" J.Biol.Chem.272(7). 3883-3886 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mineko Maeda: "Activation of the Mitogen-activated Protein Kinase ERK2 by the Chemoattractant Folic Acid in Dictyostelium" J.Biol.Chem.272(38). 23690-23695 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamao Saito: "Fatty Acid Composition of the Cellular Slime Mold Polysphondylium pallidum" Lipids. 33(3). 327-332 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahiro Morio: "The Dictyostelium Developmental cDNA Project : Generation and Analysis of Expressed Sequence Tags from the First-Finger Stage of Development" DNA Research. 5. 335-340 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Barbara Peracino: "G Protein β Subunit-null Mutants Are Impaired in Phagocytosis and Chemotaxis Due to Inappropriate Regulation of the Actin Cytoskeleton" J.Cell Biol.141. 1529-1537 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Eleonora Ponte: "Detection of subtle phenotypes : The case of the cell adhesion molecule csA in Dictyostelium" Proc.Natl.Acad.Sci.9360-9365 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maurizia Valli: "β-1, 3-galactosyltransferase and α-1, 2-fucosyltransferase involved in the biosynthesis to type-1-chain carbohydrate antigens in human colon adenocarcinoma cell lines" Eur.J.Biochem. 256. 494-501 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Davide Zaccaria: "UGUS, a reporter for use with destabilizing N-termini" Nucleic Acid Res.26. 1128-1129 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Min Pi: "Characterization of a novel small RNA encoded by Dictyostelium discoideum mitochondrial DNA" Mol.Gen.Genet.257. 124-131 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miho Iijima: "Expression of Starvation -Induced Genes in Zygotes of Dictyostelium discoideum" J.Plant Res.111. 93-96 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masao Iwamoto: "A ribosomal protein gene cluster is encoded in the mitochondrial DNA of Dictyostelium castellanii" Curr Genet.33. 304-310 (199W)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miho Iijima: "A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development" Gene. 213. 101-106 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Ochiai: "Dictyostelium-A Model System for Cell and Developmental Biology (University Academic Press, Tokyo) (Eds.Y.Maeda, K.Inouye, and I.Takeuchi)" Cell-Cell Adhesion Protein gp64 of Polysphondylium pallidum, 15 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mineko Maeda: "Dictyostelium-A Model System for Cell and Developmental Biology (University Academic Press, Tokyo) (Eds.Y.Maeda, K. Inouye, and I.Takeuchi)" Aggregation-Related Protein Kinases in Dictyostelium, MAP-kinase ERK2 and cAMP-Dependent Protein Kinase PKA, 11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 前田ミネ子: "蛋白質核酸酵素" 日本におけるヒト・ゲノム研究の最前線、細胞性粘菌, 6 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 斉藤玉緒: "生化学" 細胞性粘菌Polysphondylium pallidumの膜蛋白質gp64の構造と機能に関する最近の知見」(総説), 5 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 落合 廣: "実験医学" 華麗な粘菌さんは今日も行く!, 1 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 舟木 聡: "細胞工学別冊(野村慎太郎、渡邊俊樹監修:『脱アイソトープ実践プロトコール』,秀潤社、東京)" 増感化学発光を用いたサザンハイブリダイゼイション, 10 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Ochiai: "Dimer formation of a putative cell-cell adhesion protein" Plant Cell Physiol. 37 (2). 135-139 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoru Funamoto: "Antisense RNA inactivation of gene expression of a cell-cell adhesion protein (gp64) in the cellular slime mold Polysphondylium pallidum" J.Cell Sci.109. 1009-1016 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Satoru Funamoto: "Simple detection methods of mRNA and its complementary antisense RNA transcripts." Seikagaku. 68 (4). 303-305 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamao Saito: "Identification of a novel all-cis-5,9,12-heptadecatrienoic acid in the cellular slime mold Polysphondylium pallidum" Lipids. 31. 445-447 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuyuki Nakata: "Inhibitory effects of tunicamycin on cell-cell adhesion and cell reaggregation of the cellular slime mold Polysphondylium pallidum" J.Plant Res.109. 193-199 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masashi Fukuzawa: "Molecular cloning and characterization of the cDNA for discoidin II of Dictyostelium discoideum." Plant Cell Physiol.37. 505-514 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Marco Trinchera: "Dictyostelium cytosolic fucosyl-transferase synthesizes H type 1 trisacccharide in vitro" FEBS Letters. 395. 68-72 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Francisco Rivero: "The role of the cortical cutoskeleton : F-actin crosslinking proteins protect against osmotic stress, ensure cell size, cell shape and motility, and contribute to phagocytosis and development." J.Cell Sci.109. 2679-2691 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nobuyuki Yamaguchi: "Targeted disruption of genes for gp138, a cell-fusion-related protein in Dictyostelium discoideum, revealed the existence of a third gene." Develop.Growth Differ.38. 271-279 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Min Pi, Yoshimasa Tanaka: "Analysis of a tRNA Gene-Like Sequence (t-Element) with TTA at the Anticodon Position in the Mitochondrial DNA of Dictyostelium discoideum" J.Plant Res.109. 1-6 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Enrico Bracco: "Cloning and transcriptional regulation of the gene encoding the vacuolar/H^+ ATPase B subunit of Dictyostelium discoideum." FEBS Letters. 419. 37-40 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kazuhiro Aiba: "Isoforms of gp138, a Cell-Fusion Related Protein in Dictyostelium discoideum" J.Biochem.121. 238-243 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shinji Ogawa: "Group-introns in the cytochrome oxidase genes of Dictyostelium discoideum : two related ORFs in one loop of a group-intron, a cox1/2 hybrid gene and an unusually large cox3 gene" Curr.Genet.31. 80-88 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kayoko Komori: "Cloning and characterization of the gene encoding a mitochondrially localized DNA topoisomerse II in Dictyostelium discoideum" Biochi, Biophys.Acta. 1352. 63-72 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kayoko Komori: "Localization of a DNA Topoisomerase II to Mitochondria in Dictyostelium discoideum : Deletion Mutant Analysis and Mitochondrial Targeting Signal Presequence." J.Plant Res.110. 65-75 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shinji Ogawa: "A site-specific endonuclease specified by one of two ORFs encoded by a group I intron in Dictyostelium discoideum mitochondrial DNA." Gene. 191. 115-121 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yuzuru Kubohara: "Efficient induction by DIF-1 and 8-bromo Cyclic AMP of Prespore-to-Stalk Conversion in Dictyostelium discoideum" Comp.Biochem.Physiol.118 (3). 841-845 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Laurence Aubry: "The Dictyostelium Mitogen-activated Protein Kinase ERK2 Is Regulated by Ras and cAMP-dependent Protein Kinase (PKA) and Mediates PKA Function." J.Biol.Chem.272 (7). 3883-3886 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Mineko Maeda: "Activation of the Mitogen-activated Protein Kinase ERK2 by the Chemoattractant Folic Acid in Dictyostelium" J.Biol.Chem.272 (38). 23690-23695 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamao Saito: "Fatty Acid Composition of the Cellular Slime Mold Polysphondylium pallidum." Lipids. 33 (3). 327-332 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahiro Morio: "The Dictyostelium Development cDNA Project : Generation and Analysis of Expressed Sequence Tags from the First-Finger Stage of Development" DNA Research. 5. 335-340 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Barbara Peracino: "G Protein beta Subunit-null Mutants Are Impaired in Phagocytosis and Chemotaxis Due to Inappropriate Regulation of the Actin Cytoskeleton" J.Cell Biol.141. 1529-1537 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Eleonora Ponte: "Detection of subtle phenotypes : The case of the cell adhesion molecule csA in Dictyostelium" Proc.Natl.Acad.Sci.95. 9360-9365 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Maurizia Valli: "beta-1,3-galactosyltransferase and alpha-1,2-fucosyltransferase involved in the biosynthesis to type-1-chain carbohydrate antigens in human colon adenocarcinoma cell lines" Eur.J.Biochem.256. 494-501 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Davide Zaccaria: "UGUS,a reporter for use with destabilizing N-termini" Nucleic Acid Res.26. 1128-1129 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Min Pi: "Characterization of a novel small RNA encoded by Dictyostelium discoideum mitochondrial DNA" Mol.Gen.Genet.257. 124-131 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miho Iijima: "Expression of Starvation-Induced Genes in Zygotes of Dictyostelium discoideum." J.Plant Res.111. 93-96 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Masao Iwamoto: "A ribosomal protein gene cluster is encoded in the mitochondrial DNA of Dictyostelium castellanii" Curr Genet.33. 304-310 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Miho Iijima: "A Dictyostelium discoideum homologue to Tcp-1 is essential for growth and development" Gene. 213. 101-106 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hiroshi Ochiai: "Cell-Cell Adhesion Protein gp64 of Polysphondylium pallidum" Dictyostelium-A Model System for Cell and Development Biology. Eds., Y.Maeda, K.Inouye, and I.Takeuchi, University Academic Press, Tokyo. 15 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
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  • [Publications] Mineko Maeda: "Aggregation-Related Protein Kinases in Dictyostelium, MAP-kinase ERK2 and cAMP-Development Protein Kinase PKA" Dictyostelium-A Model System for Cell and Development Biology. Eds., Y.Maeda, K.Inouye, and I.Takeuchi, University Academic Press, Tokyo. 11 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
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  • [Publications] Maeda Mineko: "The Cellular Slime Mold" Frontiers of human genome study in Japan. Tanpakushitsu Kakusan Koso. 6 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Saito Tamao: "Recent progress of Study of the membrane protein gp64 of Polysphondylium pallidum (review)" Seikagaku. 5 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
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  • [Publications] Ochiai Hiroshi: "Dancing of Cheerful Cellular Slime Molds (A short review)" Experimental Medicine. 1 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
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  • [Publications] Funamoto Satoru: "Southern Hybridization by Enhanced Chemiluminescence" Cell Engineering (Syujun Co.Limited), Supplement. 10 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
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  • [Publications] Takahiro Morio: "The Dictyostelium Development cDNA Project:Generation and analysis of Expressed Sequence Tags from the First-Finger Satage of Development" DNA Research. 5. 335-340 (1998)

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  • [Publications] Tamao Saito: "Fatty Acid Composition of the Cellular Slime Mold Polysphondylium pallidum" Lipids. 33. 327-332 (1998)

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  • [Publications] Masaru Funamoto: "Overexpression of a dominant negative regulatory subunit of PKA leads to formation of no branching fruiteing bodies in Polysphondylium pallidum" Developmental Biology. (in press). (1999)

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  • [Publications] 落合 廣: "華麗な粘菌さんが今日も行く" 実験医学. 16. 1689-1689 (1998)

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  • [Publications] Tamao Saito: "Fatty acid composition of the celluular slime mold Polyspondylium pallidum" Lipids in press. (1998)

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  • [Publications] 船木 聡: "増感化学発光を用いたサザンブロッティング(非ラジオアイソトープサザン解析)" 細胞工学. (印刷中). (1998)

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  • [Publications] 斎藤 玉緒: "細胞性粘膜Polyspondylium pallidumの膜タンパク質gp64の構造と機能に関する最近の知見" 生化学. (印刷中). (1998)

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  • [Publications] Hiroshi Ochiai: "A Model System for Cell and Developmental Biology (eds.Y.Maeda,K.Inouye and I.Takeuchi)," University Academic Press,Tokyo,488 (1997)

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  • [Publications] Saito,T.: "Identification of a novel all-cis-5,9,12-heptadecatrienoic acid in the cellular slime mold Polysphondylium pallidum." Lipids,. 31. 445-447 (1996)

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  • [Publications] Funamoto,S.: "Antisence RNA inactivation of gene expression of a cell-cell adhesion protein (gp64) in the cellular slime mold Polysphondylium pallidum." J.Cell.Sci.37. 1009-1016 (1996)

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  • [Publications] Fukuzawa,M.: "Molecular cloning and characterization of the cDNA for Discoidin II of Dictyostelium discoideum." Plant Cell Physiol.37. 505-514 (1996)

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  • [Publications] Ochiai,H.: "Dimer formation of Tunicamycin on cell-cell adhesion protein,gp64 of the cellular slime mold,Polysphondylium pallidum." Plant Cell Physiol.37. 135-139 (1996)

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  • [Publications] Nakata,N.: "Inhibitory Effects of Tunicamycin on Cell-Cell Adhesion and Cell Reaggregation of the Cellular Slime Mold,Polysphondylium pallidum." J.Plant Res.109. 193-199 (1996)

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  • [Publications] Rivero,F.: "The role of the cortical cytoskeleton : F-actin cross-linking proteins protect against osmotic stress,ensure cell size,cell shape and motility." J.Cell Sci.(in press).

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  • [Publications] Trichera,M.: "Dictyostelium cytosolic fucosyltransferase synthesizes H type I trisaccharide in vitro" FEBS letter.,in press.

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  • [Publications] Schuster,S.C.: "The hybrid histidine kinase Dok A is part of the osmotic response system of Dictyostelium" EMBO J.,in press.

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  • [Publications] Rivero,F.: "Dictyostlelium cells lacking the 34,000 dalton actin binding protein can grow,locomote,and devekop,but exhibit defects in regulation of cell structure and movement : a case of partial redundancey" J.Cell Biol.135. 965-980 (1996)

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  • [Publications] Behirisch,A.: "The actin-binding protein hisacatophilin binds in vitro to partially charged membranes and mediates actin coupling to membranes" Biochemistry,in press.

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  • [Publications] Janssen,K.-P.: "Viscoelastic properties of F-actin solutions in the presence of normal and mutated actin-binding proteins" Arch.Biochem.Biophys.,in press.

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  • [Publications] Eichinger,L.: "Mechanical perturbation elicits a phenotypic difference between Dictyostelium wild-type cells and cytoskeleton mutants" Biophysical Journal,in press.

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  • [Publications] Maeda,M.: "Seven helix chemoattractant receptors transiently stimulate mitogen-activafed protein kinase in Dictyostelium." J.Biol.Chem.271. 3351-3354 (1996)

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  • [Publications] Aubry,L.: "The Dictyostelium MAP kinase ERK2 is regulated by Ras and cAMP-depen-dent protein kinase (PKA) and mediates PKA function." J.Biol.Chem.in press.

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  • [Publications] Jung,E.: "Linking microfilaments to intracellular membranes : the actin-binding and vesicle associated protein comitin exhibits a mannose-specific lectin activity" EMBO J.,in press.

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Published: 1996-04-01   Modified: 2016-04-21  

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