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Molecular basis of cellular functions in Dictyostelium

Research Project

Project/Area Number 08044189
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Developmental biology
Research InstitutionGraduate School of Science

Principal Investigator

MAEDA Yasuo  Tohoku University, Graduate School of Science, Professor, 理学研究科, 教授 (50025417)

Co-Investigator(Kenkyū-buntansha) VERON Michael  Division of Biochemistry, Pasteur Institute, Professor, 生化学部, 教授
FIRTEL Richard A  Center of Molecular Genetics, California University, Professor, サンジエゴ校・分子遺伝センター, 教授
WILLIAMS Jeffrey  MRC Laboratory, University College of London, Professor, MRC研究所, 教授
SUTOH Kazuo  University of Tokyo, Graduate School of Artsand Sicences, Professor, 大学院・総合文化研究科, 教授 (20111453)
TAKEUCHI Ikuo  Okazaki National Research Institute, President (90025239)
VERON Michel  バスツール研究所, 生化学部, 教授
FIRTEL Licha  カリフォルニア大学(サイジェゴ校), 分子遺伝センター, 教授
足立 博之  東京大学, 大学院・総合文化研究科, 助手 (00211699)
雨貝 愛子  東北大学, 大学院・理学研究科, 助手 (90261552)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥4,900,000 (Direct Cost: ¥4,900,000)
Fiscal Year 1997: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1996: ¥2,600,000 (Direct Cost: ¥2,600,000)
Keywordscell cycle / growth / differentiation / pattern formation / cytokinesis / chemotaxis / starvation / Dictyostelium / 細胞性粘菌 / パたーン形成 / 飢餓
Research Abstract

(1) Using the good method for cell synchnony and doubly transformed Dictyostelium cell populations, it has been demonstrated that there is a strong correlation between ceII cycle position at the time of starvation and subsequent expression of a marker of tip differentiation. CeIIs starved in very late-G2 phase selectively differentiated into tip prestalk cells, while cells starved in mid-late G2 phase into the posterior prespore cells. Thus a combination of cell cycle stage and positional information dictates cell fate.
(2) Nocodazole- or calyculin A-treated ceIIs, whose growth around the early aggregate (mound) stage was markedly inhibited, exhibited abnormal morphogenesis to form a stick-like multicellular structure, and prespore differentiation was greatly suppressed. Accordingly, the cell-cycle progression around the mound stage seems to be required for prespore differentiation.
(3) The signals for starvation response have been shown to be transduced through elevated [Ca^<2+>] i. Also, some phosphoproteins responsible for starvation response were found.
(4) Three genes ware isolated and identified as ones specifically expressed during the transition of Dictyostelium cells from growth to differentiation.
(5) Dictyostelium IQGAP-related protein has been shown to be specifically involved in the completion of cytokinesis. In addition, sliding of actin filaments was found to be greatly modulated by mutations of the SH2 cystcine in Dictyostelium myosin ll.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Tsuyoshi Araki: "Symmetry breaking in Dicfyostelium mophogenesis: Evidenc that a combination of cell cycle stage and positicnal information" Developmental Biology. 192. 645-648 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuyoshi Araki: "Mutual relation between the cell-cycle progression and prespore differentiation in Dicfyostelium development" Zoological Science. 15. 67-74 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yukiko Tanaka: "The signals for starvation response are transduced through elevaled [Ca^<2+>]i in Dicfyostelium cells" Experimental Cell Research. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Soo-cheon Choe: "Preferential expression of the cDNA emoding the proteasome subunit during the growth/differentiation fransition" Biochemical and Biophysical Research Communications. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroyuki Adachi: "Dicfyostelium IQGAP-related protein specifically involved in the completion of cttopeinesis" Journal of Cell Biology. 137. 891-898 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akira Nagasaki: "A novel Dicfyostelium disaideum gene required for cAMP-dependent cell aganegation" Biochemical and Biophysical Research Communications. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuo Maeda: "Dicfyortelium : A Model System for Cell and Developmental Biology" Universal Academy Press, Tokyo, 488 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amagai, A.and Maeda, Y.: "Dictyostelium mucoroides cells exhibit cell cycle-dependent sorting behavior as observed in D.discoideum" Dev.Growth Differ.38. 33-40 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Arakane, T.and Maeda, Y.: "Relevance of histone H1 kinase activity to the G2/M transition during the cell cycle of Dictyostelium discoideum" J.Plant Res.110. 81-85 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Okafuji, T., Abe, F.and Maeda, Y.: "Antisense-mediated regulation of Annexin Vll gene expression during the transition from growth to differentiation in Dictyostelium discoideum" Gene. 189. 49-56 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Araki, T., Abe, T., Williams, J.and Maeda, Y.: "Symmetry breaking in Dictyostelium morphogenesis : evidence that a combination of cell cycle stage and positional information dictates cell fate" Dev.Biol.192. 645-648 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sawada, Y., Maeda, Y., Takeuchi, I., Williams, J.and Maeda, Y.: "Rapid differentiation of Dictyostelium discoideum cells under confined geometry and its relation to differentiation" Dev.Growth Differ.40. 113-120 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Araki, T.and Maeda, Y.: "Mutual relation between the cell-cycle progression and prespore differentiation in Dictyostelium development" Zool.Sci.15. 67-74 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Chae, S.-C.and Maeda, Y.: "Preferential expression of the cDNA encoding the proteasome subunit during the growth/differentiation transition of Dictyostelium cells" Biochem.Biophys.Res.Commun.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tanaka, Y., Itakura, R., Amagai, A.and Maeda, Y.: "The signals for starvation response are transduced through elevated [Ca^<2+>] i in Dictyostelium cells" Exp.Cell Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yumura, S., Furuya, K.and Takeuchi, I.: "Intracellular free calcium responses during chemotaxis of Dictyostelium cells" J.Cell Sci.109. 2673-2678 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aizawa, H., Sutoh, K.and Yahara, I.: "Overexpression of cofilin stimulates bundling of actin filaments, membrane ruffling and cell movement in Dictyostelium cells" J.Cell Biol.132. 335-344 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, H., Takahashi, Y., Hasebe, T., Shirouzu, M., Yokoyama, S.and Sutoh, K.: "Dictyostelium IQGAP-related protein specifically involved in the completion of cytokinesis" J.Cell Biol.137. 891-898 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shimada, T.Sasaki, N., Ohkura, R.and Sutoh, K.: "Alanine scanning mutagenesis of switch 1 region of the ATPase site of Dictyostelium discoideum myosin ll" Biochemistry. 36. 14037-14043 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nagasaki, A., Sutoh, K., Adachi, H.and Sutoh, K.: "A novel Dictyostelium discoideum gene required for cAMP-dependent cell aggregation" Biochem.Biophysi.Res.Commun.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tsuyoshi Araki: "Symmeery breaking in Dictyosleliam oncrphogenesis:Evicleme det a combination of cell cyul stage and positicxal infinnation dictates cell fate" Developmental Biology. 192. 645-648 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tsuyoshi Araki: "Mutual relation between the cell-cyclc progression and Prespcre differentiatlon in Pictyoslelium olevelopment" Zoological Science. 15. 67-74 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yukiko Tanaka: "The signals for starvation respcrse are fransduced through elevatool 〔Ca^<2+>〕i in Dictyoslelium cells" Experimental Cell Research. 239in (press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Soo-cheon choe: "Prefevential expression of the CDNA encoking the proteasome subunit during ethe grower/ofifferentiation fransitcox in Dictyosleliccm" Biochemical and Bifophysical Reseanch Communication. (in Press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroyuki Adachi: "Dictyostelium IQGAP-nelated pnotein specifically in volved in the completion of cytokinesis" Journal of Cell Biology. 137. 891-898 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akira Nagasaki: "A novel Dirfyostelium discoideum gene rewired for c-AMP. dependent all aggegafom" Biochemical and Biophysical Researcl Cenmunication. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Maeda, Y.: "Universal Academy Press,Tokyo" Dicfyoscelium:A Model system for cell and Developmental Biology. 1997. (488)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aiko Amagai: "Dictyostelium mucoroides cells exhibit cell cycle-dependent sorting behavior as observed in D. discoideum development" Development,Growth & Differentiation. 38. 33-40 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tatsuya Okafuji: "Antisense-mediated regulation of Annexin VII gene expression during the transition from growth to differentiation in Dictyostelium discoideum" Gene. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Tohru Arakane: "Relevance of histone H1 kinase activity to the G2/M transition during the cell cycle of Dictyostelium discoideum" Journal of Plant Research. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shigehiko Yumura: "Intracellular free calcium responses during chemotaxis of Dictyosteliam cells" Journal of Cell Science. 109. 2673-2678 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Fujita: "Characterization of mutant myosins tquivalent to human familial hypertophic cardiomyopathy mutants" Journal of Clinical Investigation. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] J.Qu: "The tol Z gene of Escherichia coli is identified as the fts H gene" Journal of Bacteriology. 178. 3457-3461 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 雨貝愛子(部分執筆): "図解生物科学講座(3)「発生生物学」(浅島誠編著)植物の発生学" 朝倉書店, 182 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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