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STUDIES ON THE MANIFOLD OF PHYSIOLOGICAL FUNCTION OF AVIAN GAMETES

Research Project

Project/Area Number 08044214
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Zootechnical science/Grassland science
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

FUJIHARAN Noboru  Kyushu Univ., Fac.of Agric., Professor, 農学部, 教授 (60150512)

Co-Investigator(Kenkyū-buntansha) BLESBOIS Elisabeth  Station de Recherches Avicole, Poult.Reprod.Chief, 主任研究員
BRILLARD Jean-Pierre  Station de Recherches Avicole, Poult.Reprod.Head, 繁殖研究部長
SOH Tomoki  Kyushu Univ., Fac.of Agric., Assist.Professor, 農学部, 助手 (90221340)
HATTORI Masaaki  Kyushu Univ., Fac.of Agric., Assoc.Professor, 農学部, 助教授 (60175536)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1997: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsChiken spermatozoa / Oviducts / Fatty acids / Fertilizability / In vitro storage / Vectors / Artificial insemination / Transgenic / 外来遺伝子 / X-gal染色 / 鶏胚 / PCR法 / 生殖巣 / 始原生殖細胞 / 過酸化脂質
Research Abstract

In this research project, particular attention was paid to some of physiological function of chicken spermatozoa in order to clarify the mechanism by which sperm cells probably improved their fertilizing ability when imseminated following in vitro preservation or after intra-vaginal insemination with artificial insemination. Some of the results important for chicken fertilization are as follows ;
1) Life span of sperm cells preserved in the utero-vaginal sperm host glands of the hens was probablyl controlled by genetic strains and/or the age of hens (Brillard).
2) Changing of fatty acids in the diets induced some kinds of changes of semen and sperm cells, resultsing in the alteration of ferilizing ability of spermatozoa (Blesbois).
3) Possible alteration of amino acid compositions of rooste feed having reduced protein level brought about some changes of ATP content of sperm cells (Fujihara).
4) Upon preservation of spermatozoa outside the body, uterine fluids were proven to be harmful to the physiological functions of sperm cells (Blesbois).
5) A kind of hybrid foreign DNA, MiwZ was examined for sperm vectors so as to develop some transgenic chicken via sperm vectors carrying exogenous genes (Fujihara).
6) For artificial insemination of vian species other than chicken, two kinds of methos, deep vaginal insemination and finger-guided insemination, were compared (Brillar).

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Blesbois E.: "Effect of dietary fat on the fatty acid composition and fertillizing ability of fowl semen." Biol.Reprod.56. 1216-1220 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Blesbois E.: "Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa." Theriogenol.43. 1351-1358 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Brillard J.P.: "Mule duck production:A matter to technique to adequately inseminate pekin female ducks?" Proc.XXth World Poult.Congr.India,. 1. 547-554 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Brillard J.P.: "Sperm storage and release in fowl hens as related to either type of bird,age or physiological status." Proc.11th Europ.Symp.Waterfowl,France. 1. 8-10 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujihara,N.: "Physiological comparison of semen and spermatozoa in birds" Curent Topics in Pharmacol.3. 59-65 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujihara,N.: "Artificial control of membrane function of rooster spermatozoa." J.Appl.Anim.Res.,. 11. (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujihara,N.: "Artificial control of membrane function of rooster spermatozoa." J.Appl.Anim.Res.,. 11. (1997)137-144

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Brillard.J.P.: "Poultry Production,." Elsevier,Co.Ltd., 11 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujihara,N.: "Reproductive BioIogy Update" Nakashima Pub Print.Co.Ltd., 13 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Blesabois, E.: "Effect of dietary fat on the fatty acid composition and fertilizing ability of fowl semen." Biol.Reprod.56. 1216-1220 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Blesbois, E.: "Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa." Theriogenol. 43. 1351-1358 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Brillard, J.P.: "Mule duck production : A matter to technique to adequatly inseminate pekin female duck?" Proc.XXth World Poult.Congr.India. Vol.1. 547-554 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Brillard, J.P.: "Sperm storage and release in fwol hens as related to either type of bird, age or physiological status." Proc.11th Europ.Symp.Waterfowl, France. Vol.1. 8-10 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujihara, N.: "Physiological comparison of semen and spermatozoa in birds." Current Topics Pharmacol.3. 59-65 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujihara, N.et al.: "Artificial control of membrane function of rooster spermatozoa" J.Appl.Anim.Res.11. 137-144 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Brillard, J.P.: Mating and Fertility. Poultry Production, Elsevier. Elsevier, Co.Ltd., 271-282 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Fujihara, N.: In vitro gene transfer to chick embryos via spermatozoa and primordial germ cells. Reparoductive Biology. Nakashima Print.Co.Ltd., 389-401 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Blesbois E.et al.,: "Effect of dietary fat on the fatty acid composition and fertilizing ability of fowl semen." Biol.Reprod.56・4. 1216-1220 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Blesbois E.et al.,: "Effects of the freezing rate on viability and fertility of frozen-thawed fowl spermatozoa." Theriogenol.43・3. 1351-1358 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Brillard J.P.et al.: "Mule duck production : A matter to technique to adequately inseminate pekin female ducks?" Proc.XXth World Poult.Congr.India,. 547-554 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Brillard J.P.et al.: "Sperm storage and release in fowl hens as related to either type of bird,age or physiological status." Proc.11th Europ.Symp.Waterfowl,France. 8-10 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujihara, N.: "Physiological comparison of semen and spermatozoa in birds" Curent Topics in Pharmacol.3・1. 59-65 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujihara, N.et al.: "Artificial control of membrane function of rooster spermatozoa." J.Appl.Anim.Res.,. 11・2. 137-144 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Brillard J.P.et al.: "Poultry Production" Elsevier,Co.Ltd., 11 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujihara, N.et al.: "Reproductive Biology Update" Nakanishi Print.Co.Ltd., 13 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Fujihara,N.,Zhao,J and Hattori,M.: "Artificial control of membrane function of rooster spermatozoa" Journal of Applied Animal Research. 11(1). 23-31 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujihara,N.: "Physiological comparison of semen and spermatozoa in birds" Current Review of Pharmacology. 4(1). 17-34 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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