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Myosin-establishing its role and location in plant cells

Research Project

Project/Area Number 08044219
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field 植物生理
Research InstitutionInternational Christian University

Principal Investigator

RIDGE Robert  International Christian University Department of Biology, Associate Professor, 教養学部, 助教授 (10211666)

Co-Investigator(Kenkyū-buntansha) SCHWARTZ O  Australian National University, Research School of Biol.Sciences, Research Fello, Research F
WILLIAMSON Re  Australian National University, Research School of Biol.Sciences, Professor, 教授
PLAZINSKI J  Australian National University, Research School of Biol.Sciences, Senior Researc, Senior Res
SHIMMEN Teruo  Himeji Institute of Technology, Department of Life Science, Professor, 理学部, 教授 (80114510)
WILLIAMSON E  オーストラリア国立大学, 教授
PKIZINSKI Ja  オーストラリア国立大学, Senior Res
OWEN Schwart  オーストラリア国立大学, Senior Res
RICHARD Will  オーストラリア国立大学, Senior Fel
JACEK Plazin  オーストラリア国立大学, Senior Res
SCHWARTZ Ow  オーストラリア国立大学, Senior Res
PLAZINSKI J  オーストラリア国立大学, Senior Res
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥21,000,000 (Direct Cost: ¥21,000,000)
Fiscal Year 1998: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1997: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1996: ¥7,000,000 (Direct Cost: ¥7,000,000)
KeywordsMyosin / Actin / Cytoplasmic streaming / Monoclonal antibodies / Anti-sense plants / Endoplasmic reticulum / Arabidopsis / Green fluorescent protein
Research Abstract

Over the last three years we have investigated the role and location of plant myosin in plant cells. During this period we have isolated five myosin isotypes, purified many homozygous lines of anti-sense plants, and have inserted the green fluorescent protein DNA from the jellyfish Aqueorus into Arabidopsis plants. We have been able to analyze the act in-myosin-based movement of endoplasmic reticulum using the confocal laser microscope in developing cells of the root and hypocotyl. We have published a number of publications related to this work in international journals including Science, Protoplasma, Plant Physiology and Plant Cell Physiology. Currently we have submitted several papers to journals such as Plant Cell. We consider this project a large success and will continue to research this major area of basic research in plants.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Dev't of a Monitoring Apparatus." Plant Cell Physiol.37. 591-597 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rolfe B.G., R.W.Ridge et al.: "Defective infection and nodulation of clovers by exopolysaccharide mutants of Rhizobium leguminosarum bv.trifolii." Australian J.Plant Physiol.23. 285-303 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Collings D.A., et al.: "Actin-microtubule interactions in the alga Nitella : analysis of the mechanism by which microtubule depolymerization potentiates cytochalasin's effects on cytoplasmic streaming" Protoplasma. 191. 178-190 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Plazinski J.et al.: "Myosin from angiosperms, ferns and algae : amplification of gene fragments with PCR primers, detection of protein products with a monoclonal antibody to a conserved head epitope." Protoplasma. 196. 78-86 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tominaga, M.et al.: "Microtubules regulate the organization of actin filaments at the cortical region in root hair cells of Hydrocharis." Protoplasma. 199. 83-92 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Pharmacological Analysis." Plant Cell Physiol.38. 139-148 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Effects of External Ca2+ and Cl-" Plant Cell Physiol.38. 691-697 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kikuyama M., Shimmen T.: "Role of Ca2+ in Triggering Tonoplast Action Potential in Intact Nitella flexilis." Plant Cell Physiol.38. 941-944 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takabatake, R., Shimmen, T.: "Inhibition of Electrogenesis by Aluminum in Characean Cells." Plant Cell Physiol.38. 1264-1271 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Decrease in Electrical Membrane Resistance in Receptor Potentials." Plant Cell Physiol.38. 1298-1301 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kropf D.L.et al: "Microtubule orientation and dynamics in elongating characean internodal cells following cytosolic acidification, induction of pH bands, or premature growth arrest." Protoplasma. 197. 188-198 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arioli T.et al.: "Molecular analysis of cellulose biosynthesis in Arabidopsis thaliana." Science. 179. 717-720 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arioli T.et al.: "How many cellulose synthase-like gene products make cellulose?" Trends in Plant Science. 3. 165-166 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ridge, R.W.et al.: "The diversity of lectin-detectable sugar residues on root hair tips of selected legumes correlates with the diversity of their host ranges for rhizobia." Protoplasma. 202. 84-90 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tominaga M.et al.: "Mechanism of inhibition of cytoplasmic streaming by auxin in root hair cells of Hydrocharis." Plant Cell Physiol.39. 1342-1349 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakayasu, T.et al.: "Purification of an actin-binding protein composed of 115-kDa polypeptide from pollen tubes of lily." Biochem.Biophys.Res.Comm.249. 61-65 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokota, E.et al.: "Actin-bundling protein isolated from pollen tubes of lily. Biochemical and immunocytochemical characterization." Plant Physiol.116. 1421-1429 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokota, E.et al.: "Inhibitory regulation of higher-plant myosin by Ca2+ ions." Plant Physiol.119. 231-239 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmen T.: "Studies on Mechano-Perception in Characeae : Development of a Monitoring Apparatus." Plant Cell Physiol.37. 591-597 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Rolfe B.G., R.W.Carlson, R.W.Ridge, F.B.Dazzo, P.F.Mateos, C.E.Pankhurst: "Defective infection and nodulation of clovers by exopolysaccharide mutants of Rhizobium leguminosarum bv.trifolii." Australian J.Plant Physiol.23. 285-303 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Collings D.A., Wasteneys G.O., Williamson R.E.: "Actin-microtubule interactions in the alga Nitella : analysis of the mechanism by which microtubule depolymerization potentiates cytochalasin's effects on cytoplasmic streaming." Protoplasma. 191. 178-190 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Plazinski J., Elliott J., Hurley U.A., Burch J., Arioli T., Williamson R.E.: "Myosin from angiosperms, ferns and algae : amplification of gene fragments with versatile PCR primers and detection of protein products with a monoclonal antibody to a conserved head epitope." Protoplasma. 196. 78-86 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tominaga, M., Morita, K., Sonobe, S., Yokota, E.and Shimmen, T.: "Microtubules regulate the organization of actin filaments at the cortical region in root hair cells of Hydrocharis." Protoplasma. 199. 83-92 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Pharmacological Analysis." Plant Cell Physiol.38. 139-148 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Effects of External Ca2+ and Cl-" Plant Cell Physiol.38. 691-697 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kikuyama M., shimmen T.: "Role of Ca2+ in Triggering Tonoplast Action Potential in Intact Nitella flexilis." Plant Cell Physiol.38. 941-944 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takabatake, R., Shimmen, T.: "Inhibition of Electrogenesis by Aluminum in Characean Cells." Plant and Cell Physiol.38. 1264-1271 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Decrease in Electrical Membrane Resistance in Receptor Potentials." Plant Cell Physiol.38. 1298-1301 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kropf D.L., Wasteneys G.O., Williamson R.E.: "Microtubule orientation and dynamics in elongating characean internodal cells following cytosolic acidification, induction of pH bands, or premature frowth arrest." Protoplasma. 197. 188-198 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arioli T., Peng L., Betzner A., Burn J., Wittke W., Herth W., Camilleri C., Hofte H., Plazinski J., Birch R., Cork A., Glover J., Redmond J., Williamson R.E.: "Molecular analysis of cellulose biosynthesis in Arabidopsis thaliana." Science. 179. 717-772 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Arioli T., Burn J.E., Betzner A.S., Williamson R.E.: "How many cellulose synthase-like gene products make cellulose?" Trends in Plant Science. 3. 165-166 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ridge, R.W., R.Kim and F.Yoshida: "The diversity of lectin-detectable sugar residues on root hair tips of selected legumes correlates with the diversity of their host ranges for rhizobia." Protoplasma. 202. 84-90 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tominaga M., Sonobe S., Shimmen, T.: "Mechanism of inhibition of cytoplasmic streaming by auxin in root hair cells of Hydrocharis." Plant Cell Physiol.39. 1342-1349 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakayasu, T., Yokota, E.and Shimmen, T.: "Purification of an actin-binding protein composed of 115-kDa polypeptide from pollen tubes of lily." Biochem.Biophys.Res.Comm.249. 61-65 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokota, E., Takahara, K.and Shimmen, T.: "Actin-bundling protein isolated from pollen tubes of lily.Biochemical and immunocytochemical characterization." Plant Physiol.116. 1421-1429 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yokota E., Muto S., Shimmen T.: "Inhibitory regulation of higher-plant myosin by Ca^<2+> ions." Plant Physiol.119. 231-239 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tominaga,M.et al.: "Mechanism of inhibition of cytoplasmic streaming by ouxin in root hair cells of Hydrocharis." Plant Cell Physiology. 39. 1342-1349 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nakayasu,T.et al.: "Purification of an actin-binding protein composed of 115-kDa polypeptide from pollen tubes of lily." Biochem Biophys Research Comm. 249. 61-65 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yokota,E.et al.: "Actin-bundling protein isolated from pollen tubes of lily, biochemical and immunocytochemical characterization." Plant Physiology. 116. 1421-1429 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Arioli T.et al.: "How many cellulose synthase-like gene products make cellulose?" Trends in Plant Science. 3. 165-166 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Plazinski,J.et al.: "Plant myosins:phenotypic abnormalities in Arabidopsis transgenic plants that express the antisense constructs." Arabidopsis Conference,Madison,USA. (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ridge,R.W.et al.: "The diversity of lectin-detectable sugar residues on root hair tips of selected legumes correlates with the diversity of their host ranges for rhizobia." Annual Meeting of the Plant-Microbe Interactions Society of Japan.Toba City,Mie Prefecture.(1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ridge,R.W.and M.Utsuki.: "Nod factors significantly affect cytoplasmic streaming in alfalfa root hairs." Annual Meeting of the Plant-Microbe Interactions Society of Japan,Toba City,Mie Prefecture.(1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yokota,E.et al.: "Inhibitory regulation of higher-plant myosin by Ca2+ions." Plant Physiology. 119. 231-239 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ridge R.W.et al.: "GFP in living plant cells:new findings cortical ER during development" International symposium of biological nitrogen fixation:Kagoshima University. (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ridge R.W.et al.: "The diversity of lectin-detectable sugar residues on root hair tips of selected legumes correlates with the diversity of their host-ranges for rhizobia." Protoplasma. in press. (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Plazinski J.P., Ridge R.W.et al.: "Antisense expression of myosins in transgenic Arabidopsis plants leads to abnormal phenotypes." 5th International Congress of Plant Molecular Biology,Singapore.(1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Arioli A., Plazinski J., Williamson R.E.et al.: "Molecular analysis of cellulose biosynthesis in Arabidopsis." Science. 279. 717-720 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ridge, R.W., Hori T., Miyamura S.: "Analysis of flagellar movement in Ginkgo biloba sperm by high speed video microscopy" In : T.Hori,H.Tobe,R.W.Ridge,J.Tremouillaux-Guiller,W.Tulecke,P.Del Tredici,eds,Ginkgo biloba : a global treasure,Springer,Berlin.99-107 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shimmen, T.: "Studies on Mechano-Perception in Characeae : Effects of External Ca2+ and Cl-." Plant and cell physiology. 38. 691-698 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takabatake, R., Shimmen, T.: "Inhibition of Electrogenesis by Alumunum in Characean Cells." Plant and cell physiology.38. 1264-1272 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Plazinski J., Elliott J., Hurley UA., Burch J., Arioli T., & Williamson RE: "Myosins from angiosperms, ferns and algae ; amplification of genee fragments with verstile PCR primers and detection of protein products with a monoclonal antibody to a conserved head epitope." Protoplasma. 194 (in press).

    • Related Report
      1996 Annual Research Report
  • [Publications] Ridge, R. W. & M. Utsuki: "Nod factors significantly affect cytoplasmic streaming in alfalfa root hairs." Symposium on Molecular Biology of the Cytoskeleton, Joint Meeting of the Biochemistry and Molecular Biology Society and the Australian Society for Plant Physiology, Canberra, Australia.

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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