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Joint Study on Glutamic Acid Neurotransmission in the Inner Ear

Research Project

Project/Area Number 08044229
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionHirosaki University

Principal Investigator

USAMI Shinichi  hirosaki Univ., Univ.School of Medicine Associate Prof, 医学部, 助教授 (10184996)

Co-Investigator(Kenkyū-buntansha) FUJITA Shigetoshi  Hirosaki Univ., Univ.Hospital, Assistant, 医学部・付属病院, 助手 (70261451)
MATUBARA Atsushi  Hirosaki Univ., School of Medicine, Assistant, 医学部, 助手 (10260407)
オッターセン ウレ・ペッ  オスロ大学, 医学部, 教授
OTTERSEN O.P  Univ.of Oslo, School of Medicine, Prof
Project Period (FY) 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsglutamate / glutamine / NMDA / AMPA / transporter / inner ear / neurotransmitter / 神経伝達物質 / グルタミン酸 / レセプター / トランスポーター
Research Abstract

Glutamate receptors
The cellular localization of NMDA and AMPA type glutamate receptor subunits was studied in inner ear. In the spiral and vestibular ganglion, almost all cells were immunoreactive for the NMDARl subunit and the AMPA type receptor subunit GluR2/3 and 4. This indicates that both NMDA and non-NMDA type glutamate receptors may be co-distributed in the primary afferent neuronal components, and are possibly involved in neurotransmission in the primary auditory and vestibular systems.
Glutamate transporter
Glutamine synthetase
Confocal microscopy revealed that GLAST and glutamine synthetase were colocalized in supporting cells apposed to the immunonegative hair cells. Postembedding immunoelectron microscopy revealed that GLAST was heterogeneously distributed along the plasma membranes of the supporting cells, with higher concentrations basally (at the level of the afferent synapses) than apically. The present findings suggest that glutamate released at the afferent synapse of vestibular hair cells may be taken up by adjacent supporting cells and converted into glutamine. Thus, at this peripheral synapse, the supporting cells may carry out functions similar to those of glial cells in the CNS.

Report

(2 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Takumi,Y.et al.: "Discrete cellular and subcellular localization of glutamine synthetase and the glutamate transporter GLAST in the rat vestibular endorgan" Neuroscience. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Usami,S.et al.: "Differential cellular distribution of glutathione - an endogenous antioxidant - in the guinea pig inner ear" Brain Research. 743. 337-340 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Usami,S.et al.: "Aspartate is enrihed in sensory cells and fibers in the guinea pig inner ear: a quantitativeimmuneelectron microscopic analysis" Brain Research. 742. 43-49 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Usami,S.et al: "Immunocytochemical localization of taurine in the innerear." in″Taurine: Basic and Clinical Aspects″. Elsevier. 361-368 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsubara,A.et al: "AMPA receptor subunits at a glutamiate synapse: a quantitative immunogold analysis of hair cell synapses in the rat organ o Corti" J.Neuroscience. 16.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 1996: "4457-4467"

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takumi, Y.: "Discrete cellular and subcellular localization of glutamine synthetase and the glutamate transporeter GLAST in the rat vestibular endorgan." Neuroscience. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Usami, S.: "Differential cellular distribution of glutathion e-an endogenous antioxidant-in the guinea pig inner ear." Brain Res.743. 337-340 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Usami, S.et al: "Aspartate is enriched in sensory cekks and fibers in the guinea pig inner ear : a quantitative immunoelection microscopic analysis." Brain Res.742. 43-49 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Usami, S.et al: "Immunocytochemical localization of taurine in the inner ear. In R.J.Huxtabke (Ed.), Taurine : Basic and Clinical Aspects." Elsevier, Amsterdam. 361-368 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matubara, A.: "Organization of AMPA receptor subunits at a gulutamate synapse : a quantitative immunogold analysis of hair cell synapses in the rat organ of Corti." J.Neurosci. 16. 4457-4467 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Matsubara,A.et al: "Organization of AMPA receptor subunits at a glutamate synapse : A guantitatire immunogold analysis of hair cell synapsesin the rat organ of Corti" J.Neuroscience. 16. 4457-4467 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takumi,Y.et al: "Diserete cellular and subcellular localization of glutamine sypthetase and the glutamate transporter GLAST in the rat vestibular" Neuroscience. (in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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