| Project/Area Number |
08044253
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Nagoya Universily |
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Principal Investigator |
KITAJIMA Ken Nagoya Univ., School of Agri.Sci., Associate Professor, 農学部, 助教授 (80192558)
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| Co-Investigator(Kenkyū-buntansha) |
ROTH Jurgen Univ.of Zurich, Division of Pathology, Professor, 病理部門, 教授
TROY Frederic.A Univ.of California, School of Medicine, Professor, 医学部, 教授
LENNARZ William.J State Univ.of New York, Dept.Biochem.Cell Biol., Professor, 生化学細胞生物学部門, 教授
INOUE Sadako Academia Sinica (Taiwan), Inst.Biol.Chem., Professor, 生物化学研究所, 教授
MATSUDA Tsukasa Nagoya Univ., School of Agri.Sci., Professor, 農学部, 教授 (20144131)
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
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| Keywords | POLYSIALIC ACID / KDN / SULFATED GLYCAN / CELL ADHESION / FERTILIZATION / KDN-CLEAVING ENZYME / BIOSYNTHESIS OF SIALIC ACID / FUNCTION OF SIALIC ACID |
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| Research Abstract |
The objective of this research project was to elucidation of functions of acidic glycan chains of surface glycoconjugates, such as deaminoneuraminic acid (KDN), polysialic acid, and sulfated sialic acid, in cell recognition and adhesion in animal cellular systems. The following results were obtained :
1. Structure and function of acidic glycan chains in sperm-egg adhesion : (1) Oligo/polysialic acid (Neu5Gc) chains capped by a sulfate group at O-8 position of non-reducing terminus, which were shown to inhibit fertilization of sea urchin, were found to be linked to egg receptor for sperm. In sea urchin sperm, novel tetrasialyl (Neu5Ac) Glc-Cer was found, and this type of glycolipid had an delaying effect on progress of fertilization ; (2) ZP3 and ZP1,2 homologues were for the first time demonstrated to be present in avian unfertilized eggs. Involvement ofthese ZP homologues in sperm-egg interaction are now underway.
2. Regulatory mechanism for expression of surface polysialic acid- and KDN-containing glycoconjugates establishment of methods for enzymatic synthesis of neo-KDN-glycan chains : (1) Purification procedures of KDN residue specific hydrolytic enzyme, KDNase, from a bacterium were established. Combination of this enzyme and the previously developed monoclonal antibodies enabled us to identify a minute amount of KDN residues in mammalian tissues. At the same time, the presence of KDN in mammalian cells were also shown chemically by a newly developed fluorometic (DMB) method ; (2) A new oligo Neu5Ac specific monoclonal antibody was developed.Using this antibody, ubiquitous occurrence of oligosialic acid residues in various mammalian tissues were first indicated ; (3) Biosynthetic pathway of KDN monosaccharide was identified, and enzymes involved in the pathway were shown to be also related with biosynthesis of N-acylneuraminic acid monosaccharide.
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