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Joint Research on Acidic Glycoconjugates on Cell Surfaces

Research Project

Project/Area Number 08044253
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionNagoya Universily

Principal Investigator

KITAJIMA Ken  Nagoya Univ., School of Agri.Sci., Associate Professor, 農学部, 助教授 (80192558)

Co-Investigator(Kenkyū-buntansha) ROTH Jurgen  Univ.of Zurich, Division of Pathology, Professor, 病理部門, 教授
TROY Frederic.A  Univ.of California, School of Medicine, Professor, 医学部, 教授
LENNARZ William.J  State Univ.of New York, Dept.Biochem.Cell Biol., Professor, 生化学細胞生物学部門, 教授
INOUE Sadako  Academia Sinica (Taiwan), Inst.Biol.Chem., Professor, 生物化学研究所, 教授
MATSUDA Tsukasa  Nagoya Univ., School of Agri.Sci., Professor, 農学部, 教授 (20144131)
Project Period (FY) 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1996: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsPOLYSIALIC ACID / KDN / SULFATED GLYCAN / CELL ADHESION / FERTILIZATION / KDN-CLEAVING ENZYME / BIOSYNTHESIS OF SIALIC ACID / FUNCTION OF SIALIC ACID
Research Abstract

The objective of this research project was to elucidation of functions of acidic glycan chains of surface glycoconjugates, such as deaminoneuraminic acid (KDN), polysialic acid, and sulfated sialic acid, in cell recognition and adhesion in animal cellular systems. The following results were obtained :
1. Structure and function of acidic glycan chains in sperm-egg adhesion : (1) Oligo/polysialic acid (Neu5Gc) chains capped by a sulfate group at O-8 position of non-reducing terminus, which were shown to inhibit fertilization of sea urchin, were found to be linked to egg receptor for sperm. In sea urchin sperm, novel tetrasialyl (Neu5Ac) Glc-Cer was found, and this type of glycolipid had an delaying effect on progress of fertilization ; (2) ZP3 and ZP1,2 homologues were for the first time demonstrated to be present in avian unfertilized eggs. Involvement ofthese ZP homologues in sperm-egg interaction are now underway.
2. Regulatory mechanism for expression of surface polysialic acid- and KDN-containing glycoconjugates establishment of methods for enzymatic synthesis of neo-KDN-glycan chains : (1) Purification procedures of KDN residue specific hydrolytic enzyme, KDNase, from a bacterium were established. Combination of this enzyme and the previously developed monoclonal antibodies enabled us to identify a minute amount of KDN residues in mammalian tissues. At the same time, the presence of KDN in mammalian cells were also shown chemically by a newly developed fluorometic (DMB) method ; (2) A new oligo Neu5Ac specific monoclonal antibody was developed.Using this antibody, ubiquitous occurrence of oligosialic acid residues in various mammalian tissues were first indicated ; (3) Biosynthetic pathway of KDN monosaccharide was identified, and enzymes involved in the pathway were shown to be also related with biosynthesis of N-acylneuraminic acid monosaccharide.

Report

(2 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Nishino,Satoru: "Induction,localization,and purification of novel sialidase,KDNase,from Sphingobacterium multivorum." Journal of Biological Chemistry. 271・6. 2909-2913 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kitazume,Shinobu: "The occurrence of novel Novel 9-O-sulfated NeuGc-capped α2→5-O_<glycolyl> linked oligo/polyNeu5Gc chains in sea urchin egg cell surface glycoprotein" Journal of Biological Chemistry. 271・12. 6694-6791 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Inoue,Sadako: "Identification of KDN residues in mammalian tissues and human lung carcinoma cells." Journal of Biological Chemistry. 271・40. 24341-24344 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kudo,Mariko: "Characterization of the major core structures of the α2→8-linked polysialic acid-containing glycanchains present in N-CAM in embryonic chick brains." Journal of Biological Chemistry. 271・51. 32667-32677 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terada,Takaho: "Substrate specificity of rainbow trout testis CMP-KDN synthetase." European Journal of Biochemistry. 236. 852-855 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taguchi,Tomohiko: "Occurrence and precise structural analysis of highly sulfated multiantennary N-linked glycan chains derived from a fertilization-associated carbohydrate-rich glycoprotein in unfertilized eggs of Tribolodon hakonensis." European Journal of Biochemistry. 238. (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ziak,Martin: "Occurrence of poly (α2,8-deaminoneuraminic acid) in mammalian tissues." Proc.Natl.Acad.Sci.USA. 93. 2759-2763 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Terada,Takaho: "KDNase Sm catalysis initially forms a less stable anomer of KDN and is strongly inhibited by a transition-state analogue,KDN2en,but not by Neu2en5Ac." Journal of Biological Chemistry. 272・9. 5452-5456 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 北島,健: "新しいシアル酸KDNの糖鎖生物学の最近の発展" 生化学. 68・1. 44-51 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Satoru Nishino: "Induction, Localization, and purification of novel sialidase, deaminoneuraminidase (KDNase), from Sphingobacterium multivorum." J.Biol.chem.271-6. 2909-2913 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Shinobu Kitazume: "The occurrence of novel 9-O-sulfated Neu5Gc-capped alpha2*5-O_<glyeolyl>-linked oligo/poly-Neu5Gcchains in sea urchin egg cell surface glycoprotein" J.Biol.Chem.271-12. 6694-6791 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sadako Inoue: "Identification of KDN residues in mammalian tissues and human lung carcinoma cells" J.Biol.Chem.271-40. 24341-24344 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mariko Kudo: "Characterization of the major core structures of the alpha2*8-linked polysialic acid-containing glyco-proteins present in N-CAM in embryonic chick brains." J.Biol.Chem.271-51. 32667-32677 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takaho Terada: "Substrate specificity of rainbow trout testis CMP-KDN synthetase." Eur.J.Biochem.236. 852-855 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Tomohiko Taguchi: "Occurrence and precise structural analysis of highly sulfated multiantennary N-linked glycan chains derived from a fertilization-associated carbohydrate-rich glycoprotein in unfertilized eggs of Tribolodon hakonensis." Eur.J.Biochem.238. 357-367 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Martin Ziak: "Occurrence of poly (alpha2,8-deaminoneuraminie acid) in mammalian tissues." Proc.Natl.Acad.Sci.USA. 93. 2759-2763 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Takaho Terada: "A new sialidase (KDNase sm) catalysis forms a less stable anomer of KDN and is strongly inhibited by a transition-state analogue, KDN2en, but not by Neu2en5Ac" J.Biol.Chem.272-9. 5452-5456 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Ken Kitajima18GB09 : Recent Advances in Glycobiology on KDN-glycoconjugates: Seikagaku. 68-1. 44-51 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 北島,健: "新しいシアル酸KDNの糖鎖生物学の最近の発展" 生化学. 68・1. 44-51 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ziak,Martin: "Occurrence of poly(α2,8-deaminoneura minic acid)in mammalian tissues." Proc.Natl.Acad.Sci.USA. 93. 2759-2763 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishino,Satoru: "Induction,localization,and purification of novel sialidase,KDNase,from Sphinogo-bacterium multivorum." The Journal of Biological Chemistry. 271・6. 2909-2913 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kitazume,Shinobu: "The occurrence of novel Novel 9-O-sulfated NeuGc-capped α2→5-O_<glycolyl>-linked oligo/poly Neu5Gc chains in sea urchin egg cell surface glycoprotein." Journal of Biological Chemistry. 271・12. 6694-6791 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Inoue,Sadako: "Identification of KDN residues in mammalian tissues and human lung carcinoma cells." The Journal of Biological Chemistry. 271・40. 24341-24344 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kudo,Mariko: "Characterization of the major core structures of the α2→8-linked polysialic acid-containing glycanchains present in N-CAM in embryonic chick brains." Journal of Biological Chemistry. 271・51. 32667-32677 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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