• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Comparative study of immunological tolerance in the liver transplantation from cadaver and living donor

Research Project

Project/Area Number 08044278
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YAMAOKA Yoshio  Graduate School of Medicine, Kyoto University, Professor, 医学研究科, 教授 (90089102)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Masayuki  Graduate School of Medicine, Kyoto University, Assistant Professor, 医学研究科, 助教授 (30158307)
INAMOTO Takashi  College of Medical Technology, Kyoto University, Professor, 医療技術短期大学部, 教授 (10135577)
TANAKA Koichi  Graduate School of Medicine, Kyoto University, Professor, 医学研究科, 教授 (20115877)
IKAI Iwao  Graduate School of Medicine, Kyoto University, Instructor, 医学研究科, 講師 (60263084)
INOMATA Yukihiro  Graduate School of Medicine, Kyoto University, Assistant Professor, 医学研究科, 助教授 (50193628)
福本 学  京都大学, 医学研究科, 助教授 (60156809)
PICHLMAYR Ru  ハノーファー医科大学, 教授
湊 長博  京都大学, 医学研究科, 教授 (40137716)
WONIGEIT Kur  ハノーファー医科大学, 教授
山本 雄造  京都大学, 医学研究科, 助手 (70281730)
坂井 義治  京都大学, 医学研究科, 助手 (60273455)
尾崎 信弘  京都大学, 医学研究科, 助手 (50211818)
Project Period (FY) 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥11,400,000 (Direct Cost: ¥11,400,000)
Fiscal Year 1996: ¥11,400,000 (Direct Cost: ¥11,400,000)
Keywordsimmunotolerance / micro-chmerism / donor-specific gene / MHC-class 1 / NK cell / KIR
Research Abstract

Microchimerism has been suggested to play an important role in the long-term acceptance of allogenic organ grafts by transplant patients and for the maintenance of a state of donor-specific low responsiveness. In order to elucidate the kinetics of the development of chimerism we have performed a follow-up analysis in patients with living-related liver transplantation (LRLTx). Blood samples obtained during the first 6 months and at 18 months post-transplant and slin biopsies taken at one month were analysed for the presence of donor cells by PCR using donor-specific HLA-DRB1 primer pairs or primers for a Y chromosome-specific sequence. Furthermore long-term patients more than 2 years after LRLTx were studied at two different time points. In the follow-up studies donor cells could be demonstrated in the blood of all patients immediately after transplantation. After a gradual decline all patients became chimerism negative for several weeks or months. At 6 months, however, 63% of them and … More at 18 months in 66% of them donor cells had reappeared. This biphasic pattern in the development of chimerism is proposed to reflect the occurrence of different donor-derived cell populations in the recipients. The cell population of cells occurring with the second wave of chimerism is likely to have been generated by donor-derived cells with stem cell potential located in the graft or in the hematopoetic organs of the recipient after emigration from the graft. The pediatric patient population studied here received haplo-identical graft from one of their parents. Thus the degree of histocompatibility is much better than in most other liver grafted patients receiving organs from cadaveric donors matched only for organ size and ABO blood groups but not for HLA antigens. Despite this difference, the incidence of chimerism was very similar to that found in recipients of cadaveric organs. Furthermore, we did not find a clear correlation between the development of detectable chimerism in skin or blood and the clinical course or the requirement for immunosuppressions. Less

Report

(2 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Mikiko Ueda: "Development of Microchimerism in Pediatric Patients after Living-Related Liver Transplantation"Clinical Transplantation. 11. 193-200 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Kume: "Ischemic preconditioning of the liver in rats : Implications of heat shock protein induction to increase tolerance of ischemia-reperfusion injury"J. Jab. Chin. Med.. 123(3). 251-258 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Eiji Yamomoto: "Evaluating the significance of HLA class II genotype matching in living-related liver transplantation"Transplantation Immunology. 4. 144-150 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Satoh: "Energy metabolism and regeneration in transgenic mouse liver expressing creatine kinase after major hepatectomy"Gastroenterology. 110. 1166-1174 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Morimoto: "Guideline for donor selection and an overview of the donor operation in living related liver transplantation"Transplant. Int.. 9. 208-213 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Inomoto: "Changes in glucose transporter 2 and cabohydrate-metabolizing enzymes in the liver during cold preservation and warm ischemia"Transplantation. 61(6). 869-874 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Shinohara: "Direct measurement of hepatic indocyanine green clearance with near-infrared spectroscopy : Separate evalution of uptake and remonval"Hepatology. 23(1). 137-144 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Inomata: "The evolution of immunosuppression with FK506 in pediatric living-related liver transplantation"Transplantation. 61(2). 247-252 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Inomoto: "Experiences of 120 microsurgical reconstuctions of hepatic artwry in living related liver transplantation"Surgery. 119(1). 20-26 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Kiuchi: "Background of early intragraft immune activation and rejection in liver transplans recipients"Transplantation. 60(1). 49-55 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y Yamaoka: "Safety of the donor in living related liver transplantation-Analysis of 100 parental donors"Transplantation. 59(2). 224-226 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Kiuchi: "Backgrounds of Early Intragraft Immune Activation and Regection in Liver Transplant Recipients"Transplantation. 60. 49-55 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Yamaoka: "Safety of the donor in living related liver transplantation--Analysis of 100 parental donos"Transplantation. 59(2). 224-226 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Ueda: "Development of Microchimerrism in Pediatric Patients after Living-related Liver Transplantation"Clinical Transplantation. 11. 193-200 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Inomoto: "Experiences of 120 microsurgical reconstructions of hepatic artery in living related liver transplantation"Surgery. 119(1). 20-26 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] H. Shinohara: "Direct measurement of hepatic indocyaninegreen clearance with near-infrared spectroscopy : Separate evalution of uptake and removal"Hepatology. 23(1). 137-144 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Y. Inomata: "The evolution of immunosuppression with FK506 in pediatric living-realted liver transplantation"Transplantation. 61(2). 247-252 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Inomoto: "Changes in Glucose Transporter 2 and Carbohydrateme-metrabolizing Enzymes in the liver During Cold Preservation and Warm Ischemia"Transplantation. 61. 569-874 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] T. Morimoto: "Guideline for donor selection and an overview of the donor operation in living related liver transplantation"Transplant. Int.. 9. 208-213 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] S. Satoh: "Energy metabolism and regeneration in transgenic mouse liver expressing creatine kinase after major hepatectomy"Gastroenterology. 110. 1166-1174 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] E. Yamamoto: "Evaluating the significance of HLA class II genotype matching in living-related liver transplantation"Transplantation Immunology. 4. 144-150 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] M. Kume: "Ischemic preconditioning of the liver in rats : Implications of heat shock protein induction to increase tolerance of ischemia-reperfusion injury"J. Lab Clin Med. 128. 251-257 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Mikiko Ueda: "Development of Microchimerism in Pediatric Patients after Living-Related Liver Transplantation." Clinical Transplantation. in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Kume: "Ischemic preconditioning of the liver in rats:Implications of heat shock protein induction to increase tolerance of ischemia-reperfusion injury." J.lab.Cin.Med.123(3). 251-258 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Eiji Yamamoto: "Evaluating the significance of HLA class II genotype matching in living-related liver transplantation." Transplantation Immunology. 4. 144-150 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] S.Satoh: "Energy metabolism and regeneration in transgenic mouse liver expressing creatine kinase after major hepatectomy." Gastroenterology. 110. 1166-1174 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Morimoto: "Guideline for donor selection and an overview of the donor operation in living related liver transplantation." Transpant.Int.9. 208-213 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Inomoto: "Changes in glucose transporter 2 and carbohydrate-metabolizing enzymes in the liver during cold preservation and warm ischemia." Transplantation. 61(6). 869-874 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] H.Shinohara: "Direct measurement of hepatic indocyanine green clearance withnear-infrared spectroscopy:Separate evalution of uptake and removal." Hepatology. 23(1). 137-144 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Inomata: "The evolution of immunosuppression with FK506 in pediatric living-related liver transplantation." Transplantation. 61(2). 247-252 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Inomoto: "Experiences of 120 microsurgical reconstructions of hepatic artwry in living related liver transplantation." Surgery. 119. 20-26 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Kiuchi: "Background of early intragraft immune activation and rejection in liver transplant recipients." Transplantation. 60(1). 49-55 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Yamaoka: "Safety of the donor in living related liver transplantation-Analysis of 100 parental donors." Transplantation. 59(2). 224-226 (1995)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi