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Conjugal transfer of oncogenes from bacteria to animal celIs

Research Project

Project/Area Number 08044292
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Bacteriology (including Mycology)
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YOSHIDA Kazuo  HIROSHIMA UNIVERSITY Fac.Science, Biological Science, Professor, 理学部, 教授 (70022720)

Co-Investigator(Kenkyū-buntansha) KATOH A  米国マーシー病院放射線病理癌部門, 部長
NISHIKAWA Masanobu  Okayama Biological Institute, Research Scientist, 技師 (60262812)
TAKEGAMI Tsutomu  Knazawa Medical University, Assoiate Professor, 総合医学研究所, 助教授 (10113490)
KOTOH Arthur K.  Oncopathology Div., Mercy Hospital, Chief
KATOH Arthur  米国マーシー病院, 放射線病理癌部門, 部長
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥8,800,000 (Direct Cost: ¥8,800,000)
Fiscal Year 1997: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1996: ¥4,400,000 (Direct Cost: ¥4,400,000)
Keywordstrans-kindom conjugation / conjugative plasmids / oncogenes / phytooncogenes / Ti pIasmids / animal cuIture celIs / enterobacteria / agrobacteria / 細菌・動物細胞間接合 / 接合伝達 / GFP / ミトコンドリア / 大腸菌 / 酵母
Research Abstract

For trans-kingdom conjugation between prokaryotic Escherichia coli and eukaryotic animal cultured cells, we have constructed a novel plasmid, pBASGreen which consists of oriT,SV40-ori/promoter, pBR322-oriV,Ap, Neo, and GFP (green fluorescent protein) genes. The plasmid was successfully introduced into animal cultured cells, COS1 and NIH3T3 then typical green fluorescence due to the GFP reporter gene was clearly detected in the transformant celIs. However, E.coli donor celIs were unfortunately contaminant and toxic against cultured celIs. To overcome the problems, anucleated and non-dividing celIs of E.coli mini-cell mutant P678-54 was verified to be useful because it is less toxic and keeps the activity of trans-kingdom conjugation. On the basis of conjugal oncogene transfer, we also carried out oncogenes survey in enterobacteria by using Southern blotting. On the other hand, phytopathogenic bacterium Agrobacterium tumefaciens harbors a giant Ti (tumor inducing) plasmid and transfers it's T-DNA into dicots by conjugation-like process. The infected plants subsequently have tumors induced by oncogenes on T-DNA.In order to know the relationship between T-DNA transfer and trans-kingdom conjugation on the molecular basis, we analyzed the entire genome of Ti plasmid. The complete sequencing and analyzing of 203,148bp clearly indicate the independence of conjugation and T-DNA transfer system. We also found that A.tumefaciens is less toxic against animal cultured cells as well as plant celIs. The above results offer important clues on trans-kingdom conjugation between bacterial and animal celIs.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Yoshida,K.: "Construction of a novel conjugative plasmid harboring a GFP reporter gene and its introduction into animal cells by transfection and trans-kingdom conjugation." Nucl.Acid.Symp.37. 157-158 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Obuchi,M.: "Teiler's murine encephalomyelitis virus subgroup strain-specific infection in a murine macrophage-like cell line." J.Virology. 71. 729-733 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki,K.: "Genome structure of pTi-SAKURA(II):Genetic map constructed by complete DNA sequencing." Nucleic Acids Symp.37. 161-162 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida,K.: "The structure and characteristics of Japanese cherry tree-born Ti plasmid." 11:A. 976-977 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa,M.: "Trans-kingdom conjugation offers a powerful gene targeting tool in yeast." Genetic Analysis:Biomolecular Engineering. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takegami,T.: "Inhibitory effert of furanonaphthoquinone derivatives on the replication of Japanese encephalitis virus." Antiviral Research. (in press.). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida,K.: "Intertaxonic Combination versus Symbiotic Adaptation." Springer-Verlag, 530 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K.: "Construction of a novel conjugative plasmid harboring a GFP reporter gene and its introduction into animal cells by transfection and trans-kingdom conjugation." Nucl.Acid.Symp.37. 157-158 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Obuchi, M.: "Teiler's murine encephalomyelitis virus subgroup strain-specific infection in a murine macrophage-like cell line." J.Virology. 71. 729-733 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Suzuki, K.: "Genome structure of pTi-SAKURA (II) : Genetic map constructed by complete DNA sequencing." Nucleic Acids Symp.37. 161-162 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K.: "The structure and characteristics of Japanese cherry tree-born Ti plasmid." The FEBS J.11 : A. 976-977 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nishikawa, M.: "Trans-kingdom conjugation offers a powerful gene targeting tool in yeast." Genetic Analysis : Biomolecular Engineering. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takegami, T.: "Inhibitory effect of furanonaphthoquinone derivatives on the replication of Japanese encephalitis virus" Antiviral Research. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Yoshida: lntertaxonic combination versus Symbiotic Adaptation.Springer-Verlag, 530 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, K.: "Construction of a novel conjugative plasmid harboring a GFP reporter gene and its introduction into animal cells by transfection and trans-kingdom conjugation." Nucl.Acid.Symp.,. 37. 157-158 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Obuchi, M.: "Teiler's murine encephalomyelitis virus subgroup strain-specific infection in a murine macrophage-like cell line." J.Virology. 71. 729-733 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Suzuki, K.: "Genome structure of pTi-SAKURA (II) : Genetic map constructed by complete DNA sequencing." Nucleic Acids Symp.37. 161-162 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida, K.: "The structure and characteristics of Japanese cherry tree-born Ti plasmid." The FEBS J.11:A. 976-977 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nishikawa, M.: "Trans-kingdom conjugation offers a powerful gene targeting tool in yeast." Genetic Analysis:Biomolecular Engineering. (in press). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takegami, T.: "Inhibitory effect of furanonaphthoquinone derivatives on the replication of Japanese encephalitis virus." Antiviral Research. (in press). (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida, K.: "Intertaxonic Combination versus Symbiotic Adaptation." Springer-Verlag, 530 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Mahmood.T.Kimura,M.Takenaka and K.Yoshida: "The construction of novel mobilizable YAC plasmids and their behavior during trans-kingdom conjugation between bacteria and yeasts" Genetic Analysis : Biomolecular Engineering. 13. 25-31 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Kamiji,H.Ishitomi and K.Yoshida: "A novel method of mitochondrial transformation : construction of conjugative vectors and their introduction into mitochondria by trans-kingdom conjugation" Nucleic Acids Symosium Series. 35. 205-206 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Nishikawa,O.Suzuki and K.Yoshida: "The ARS-independent synthesis of DNA complementary to transfer strand in a recipient yeast during trans-kindom conjugation between Escherichia coli and Saccharomyces cerevisiae" Nucleic Acids Symosium Series. 35. 273-274 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Sawasaki,K.Inomata and K.Yoshida: "Trans-kingdom conjugation between Agrobacterium tumcfaciens and Saccharomyces cerevisiae,a bacterium and a yeast" Plant and Cell Phisiology. 37(1). 103-106 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Katoh,S.Breier,N.Stemmler.S.Specht.K.Blanock and F.D.Amico: "p53 protein expression in humman cancer carcinoma : lack of prognostic potential for recurrence of the disease" Anticancer Research. 16. 1301-1304 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] M.Obuchi,Y.Ohara and T.Takegami: "Th iler's murine encephalomyclitis virus suogroup strain-specific infection in amurine macrophage-like cell line" Jouenal of Virology. 71(印刷中). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] K.Yoshida,K.Kamiji T.Sekito,A.Mahmood and H.Ishitomi: "Intertaxionic Combination and Symbiotic Adaptation" Springer-Verlag(印刷予定), (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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