| Project/Area Number |
08044293
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
KATO Yukio Hiroshima University School of Dentistry Prof., 歯学部, 教授 (10112062)
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| Co-Investigator(Kenkyū-buntansha) |
許 恵明 白求恩医科大学, 地方病研究所, 助教授
李 廣生 白求恩医科大学, 地方病研究所, 教授
YANG Tongshu Norman Bethune University of Medical Science Prof., 地方病研究所, 教授
IWATA Hisashi Nagoya University School of Medicine Prof., 医学部, 教授 (90023796)
YAN Weiqun Hiroshima University School of Dentistry Res.Assis., 歯学部, 助手 (80274091)
LI Kuangsheng Norman Bethune University of Medical Science Prof.
XU Huiming Norman Bethune University of Medical Science Assoc.Prof.
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| Project Period (FY) |
1996
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1996: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | chondrocyte / metalloproteinase / serum / gelatinase / animal model |
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| Research Abstract |
1 The development of a Kashin-Beck Disease model (monkey) Monkeys were fed with water and food from the Kashin-Beck Disease area, and sera were obtained from these animals.
2 sera were obtained from patients with Kashin-Beck Disease.
3 Chinese and Japanese investigators discussed studies on Kashin-Beck Disease.
4 MMP-9-inducing factors in sera from patients with Kashin-Beck Disease.
Rabbit growth plate chondrocytes were incubated for 4 or 8 days in the presence of 5% control sera or 5% sera from patients with Kashin-Beck Disease. Gelatinese activity was estimated by gelatin zymography. No gelatinase activity was found in the cell layr, but MMP-2 were found in the conditioned medium. The sera from patients with Kashin-Beck Disease induced MMP-9 of 92kDa on day 4, and increased MMP-9 levels 3-to 4-fold on day 8 relative to control sera. These findings suggest that sera from patients with Kashin-Beck Disease contain factors that stimulate MMP-9 synthesis by growth plate chondrocytes.
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