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Region Feature, Control Guideline and Sueveillance of Lyme Disease

Research Project

Project/Area Number 08044310
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionUniversity of Shizuoka

Principal Investigator

YANAGIHARA Yasutake  School of Pharmaceutical Sciences, University of Shizuoka, Professor, 薬学部, 教授 (30046255)

Co-Investigator(Kenkyū-buntansha) JOHNSON R.C.  アメリカ, ミネソタ大学・医学部, 教授
FUKUNAGA Masahito  School of Pharmaceutical Sciences, Fukuyama University, Professor, 薬学部, 教授 (20132483)
MIYAMOTO Kenji  Asahikawa Medical Collge, Associate Professor, 医学部, 助教授 (30091581)
WATANABE Haruo  Bacteriology Division, National Institute of Health of Japan, , Director, 細菌部, 部長 (70142130)
KAWABATA Masato  Medical School, University of Kobe, Professor, 医学部, 教授 (30175294)
BERGSTROM Sven A  Medical School, University of Umea, Sweden, Professor
SIMOM Markus M  Division of Immunobiology, Max-Planck Institute, Germany, Professor
BARANTON Gut  Dept.of Molecular Medical Bacteriology, Pasteur Institute, France, Professor
WILSKE Bettina  Pettenkofer Institute, University of Munich, Germany, Professor
KORENBERG Edward I  Gamaleya Institute, Russian Academy of Medical Science, Russia, Professor
JOHNSON Russell C  Medical School, University of Minnesota, U.S.A., Professor
BERGSTROM S  スェーデン, ウメーオ大学・医学部, 教授
SIMON M.M.  ドイツ, マックス・ブランク研究所, 教授
BARANTON G.  フランス, パスツール研究所, 教授
WILSKE B.  ドイツ, ミュンヘン大学ペッテンコーファー研究所, 教授
KORENBERG E.  ロシア, ガマレーヤ研究所, 教授
JOHNSON R.C  アメリカ, ミネソタ大学・医学部, 教授
Project Period (FY) 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1996: ¥7,500,000 (Direct Cost: ¥7,500,000)
KeywordsLyme disease / B.burgdorferi / Transmission / Genetic analysis / Adhesion factor / Classification / B.miyamotoi / Ixodid tick / B.garinii / B.afzelii
Research Abstract

1. From Japanese ixodid ticks, new species borreliae were identified as B.miyamotoi, B.tanuki, and B.turdae by genetic analyzes of rRNA and flagellin genes.
2. Phylogenetic tree of genus Borrelia was constructed from flagellin gene sequences and B.Miyamotoi originated from I.persulcatus is related to relapsing fever borreliae.
3. Aetiologic agents of Lyme disease were B.burgdorferi sensu stricto, B.garinii and B.afzelii. B.miyamotoi, B.t anuki and B.turdae might be non-pathogenic.
4. Expression of Osp C gene of Borrelia was suggested to relate to the infection and expression of pathogenicity.
5. Lyme disease borreliae is bound to galactosyl ceramide and glucosyl ceramide present on the surface of host cells. 41kDa flagellin protein, 61kDa HSP and 67kDa unknown protein participated borrelial adhesion in the course of infection.
6. OspC gene sequence among borreliae were highly divergent. The diversity might be functional in escape from host defense mechanism.
7. Development of classification method of borreliae among the same species was succeeded by using OspC-PCR-RFLP based on the genetic diversity. This method is applicable to epidemiological study as the molecular epidemiologic marker and is able to classification of mixed culture or multi-infected patient.
8. Genetic characteristics of Lyme diseae borreliae in Far East Asia were different from European and American-type borreliae. In Japan dominant species of Lyme disease was B.garinii and suggested to be transmitted from Russia.

Report

(2 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Toshiyuki Masuzawa et al.: "Comparison of OspA serotypes for Borrelia furgdorferi sensee lato from Japan,Europe and North America." Microbiol.Immunol.40・8. 539-545 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa et al.: "Characterization of Borrelia sp.isolated from clxodes tanuki,cl.turdus and cl columnal in Japan by restriction fragment length polymor phism of rrf(5S)-rrl(23S) intergonic spacer amp con." FEMS Microbiol.Lett.142・1. 77-83 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Toshiyuki Masuzawa et al.: "Application of PCR-restriction fragment length polymor ophism analysis of OspC gone for detection of mixed culture and epidemiological typing tool of Borrelia burgdorferi sonses stricto." Clin.Diag.Lab.Immunol. 4・1. 60-63 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masahito Fukunaga et al.: "Borrflia tanuki sp.non. and Borrelia turdal sp.non.found from ixodid ticks in Japan : raptcl species identification by 16SrRNA gene-targetd PCR analysis." Microbiol.Immunol.40・11. 877-881 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masahito Fukunaga et al.: "Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease Borrelial." Int.J.Syst.Bacteriol.46・4. 898-905 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masahito Fukunaga et al.: "A pylogemetic analysis of Borrelia burgdorferi sensff lato wolater associated with Lyme disease in Japan by flagellin gene sequence determinatis" Int.J.Syst.Bacteriol.46・2. 416-421 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 柳原保武,増澤俊幸(竹田,林編): "細菌学-ライム病ボレリア-" 朝倉書店(印刷中), (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] 柳原保武(天児,南嶋編): "戸田新細菌学(31版)-スピロヘーター" 南山堂, 990 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masuzawa, T., et al.: "Presence of common antigenic epitope in outer surface protein (Osp) A and OspB of Japanese isolates identified as Borrelia garinii." Microbiol. Immunol.40. 455-458 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masuzawa, T., et al.: "Comparison of OspA serotypes for Borrelia burgdorferi sensu lato from Japan, Europe and North America." Microbiol. Immunol.40. 539-545 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masuzawa T., et al.: "Characterization of Borrelia sp.isolated from Ixodes tanuki, I.turdus, and I.columnae in Japan by restriction fragment length polymorphism of rrf(5S)-rrl(23S)intergenic spacer amplicons." FEMS Microbiol.Lett.142. 77-83 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masuzawa, T., et al.: "Characterization of Borrelia spp.isolated from tick, Ixodes tanuki and small rodents in Japan." J.Wildl.Dis.32. 565-571 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Masuzawa T., et al.: "Application of PCR-restriction fragment length polymorophism analysis of OspC gene for detection of mixed culture and epidemiological typing tool of Borrelia burgdorferi sensu stricto." Clin.Diag.Lab.Immunol.4. 60-63 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Wang, J., et al.: "Consensus sequence on the genes encoding the major outer surface proteins (OspA and OspB) of Borrelia garinii isolate." Microbiol.Immunol.41. 83-91 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukunaga, M., et al.: "Borrelia tanukii sp.nov.and Borrelia turdae sp.nov.found from ixodid ticks in Japan : rapid species identification by 16S rRNA gene-targeted PCR analysis." Microbiol.Immunol.40. 877-881 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukunaga, M., et al.: "Phylogenetic analysis of Borrelia species based on flagellin gene sequences and its application for molecular typing of Lyme disease Borreliae." Int.J.Syst.Bacteriol.46. 898-905 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Yukie Takahashi, Y., et al.: "Physical mapping of the Borrelia miyamotoi HT31 chromosome in comparison with that of Borrelia turicatae, an etiological agent of tick-borne relapsing fever." Clin.Diag.Lab.Immunol.3. 533-540 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hamase, A., et al.: "Homology of variable major protein genes between Borrelia hermsii and Borrelia miyamotoi." FEMS Microbiol.Lett.140. 131-137 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukunaga, M., et al.: "Characterization of spirochetes isolated from ticks (Ixodes tanuki, Ixodes turdus and Ixodes columne) and comparison of the sequences with those of Borrelia burgdorferi sensu lato strains." Appl.Environ.Microbiol.62. 2338-2344 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fukunaga, M., et al.: "A pylogenetic analysis of Borrelia burgdorferi sensu lato isolates associated with Lyme disease in Japan by flagellin gene sequence determination." Int.J.Syst.Bacteriol.46. 416-421 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Kenji Miyamoto et al.: "Competence of a migratory bird,red-feilied thrush(Turdus chrysolaus)、as an reservior for the Lymedisease spirochetes in Japan." Acta Tropica. 22(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshihiko Umemoto et al.: "Treponewa medium sp. nor., isolated from subgingival dental plaque." Int. J.Syst. Bacteriol.47・1. 67-72 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wang Jianhui et al.: "Consenusus sequence on the genes encooling the major outer surface protein (OspA and OspB)of Borrelia garinii isolate." Microbiol. Immunol.41・2. 83-91 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiyuki Masazawa et al: "Application of PCR-reatriction frapment length ploymopkisum sualysis of CspC gene for deteation of mixed cculture and epideuiolsgical tool of Borrelia borgdorferi sensu stucto" Clin. Diag. Lab.Immunol.4・1. 60-63 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yukita Sato et al.: "Prevaiebce of the Lyme disease spirochetes in ixodes perbuleatus and wild rodents in Far East Russia" Appl. Environ.Microbiol.62・10. 3807-3889 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiyuki Masuzawa et al.: "Characterization of Borrelia spp. isolated from tick, Ixcdes Taunki and small roalents in Japan." J.Wildl. Dis.32・4. 565-571 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiyuki Masazawa et al.: "charactarization of Borrelia sp.:solated from Ixodes tannki,I.turdus,and I.columnae in Japan by restriction fragment leugth polymorpliom of rrl(5S)-rrl(23S)intengenic spailr osplicon." FEMS Microbiol,Lett.142・1. 77-83 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiyuki Masuzawa et al.: "Comparison CspA serofyyes for Borrelia burgdorferi senou lato from Japan, Europe and North America." Microbiol. Immunol.40・8. 539-545 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Toshiyaki Masuzawa et al.: "Preseuce of common autigenic opitope in outer surface protein(Osp)A and OspB of Japanese isolates identified as Borrelia garinii." Microbiol. Immunol.40・6. 455-458 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 増澤俊幸 他: "Borrelia japonica感染が疑われるライムボレリア症の一例" 感染症雑誌. 70・3. 264-267 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fubito Ishiguro et al.: "Reservir competence of the vole,clethrionomgs rufoconus bedforaial, for Borrelia garinii or Borrelia of zelii." Microbiol. Immunol.40・1. 67-69 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Emiko Isogai et al.: "Borrelia bung doiferi sensu lato in an evalesic euvironment:wild Sika deer (cervus nippon yesoenais) with infected ticks and antiboclies." Microbiol. Immunol.40・1. 13-19 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masahito Fukunaga et al.: "Borrelia tanuki cp、nov.and borreliaturdae sp.nov. found from ixodid tieks in japan rapid species idertification by 16SrRNA gene-targeted PCR analyais." Microbiol.Immunol.40・11. 877-881 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masahito Fukunaga et al.: "Phylogonetic analysis of Borrelia species based on flagellin gene sequences and its opplication for molecular typing of Lyme disease Borrelioe." Int. J. Syst. Bacteriol. 46・4. 898-905 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yukie Takahashi et al.: "Phyaical mapping of the Borrelia miyamotoi HT31 chromoosome in comparison with that of Borreliu turicatae, an etiological agent of tick-borne reltpsing feber." Clin. Diag. Lab. Immunol.3・5. 533-540 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akiko Hanase et al.: "Homology of variable major protein genes between Borrelia hermsii and Borrelia miyamotoi." FEMS Microbiol. Lett.140・2ー3. 131-137 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masahito Fukunaga et al.: "Characterization of spirocheter isolated from ticks(Ixodes tanuki,ixode,turdus and ixodes colunmae) and conparison of the sequence with thosed of Borrelia burg darferi senar lato strains" Appl. Environ. Microbiol.62・7. 2338-2344 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masahito Fukunaga et al: "A phyloagnetic analysis of Borrelia burgdater senou lato isolates associated with Jyme lisease in Japan by blagellin gene sequence determination." Int. J. Syst.Bacteriol.46・2. 416-421 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] T.Masuzawa, Y.Yanagihara(T.Talaska Ed.): "Borreliosen in Brandenburg -Die Erreger-Vorkommen und klinische Relevanz-" Bad Saarow(Germany)(印刷中), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 柳原保武,増澤俊幸(武田.林 編): "細菌学-ライム病ボレリア-" 朝倉書店(印刷中), (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] 柳原保武(天児,南嶋編): "戸田新細菌学(31版)-スピロヘーター" 南山堂, 990 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Yanagihara,T.Masuzawa(Tylewska-wierzbanowska Ed.): "Report of WHO Workshop on Lyme Borreliosis Diagnosis and Surveillance" World Health Ooganization, 220 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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