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Membrane inhibitors of complement in experimental animals

Research Project

Project/Area Number 08044311
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research Field Immunology
Research InstitutionNagoya City University

Principal Investigator

OKADA Hidechika  Nagoya City Univ.Sch.Med., Dep.Mol.Biol., Prof., 医学部, 教授 (30160683)

Co-Investigator(Kenkyū-buntansha) LATZKOVITS Laszlo  A.St.-Gyorgyi, Med.Univ., Neuroscience, Assoc.Prof., 助教授
MORGAN B.Paul  Univ.Wales College Med., Dep.Med.Biochem., Prof., 医学部, 教授
LAJOS Baranyi  Choju Med.Inst., Res.Fellow, 長寿医学研究所, 主任研究員 (10244542)
AKATSU Hiroyasu  Choju Med.Inst., Res.Fellow, 長寿医学研究所, 研究員
OKADA Noriko  Nagoya City Univ.Sch.Med., Dep.Mol.Biol., Asist.Prof., 医学部, 助手 (20160682)
LIPOSITZ Sz  アルバートセントジョージ医科大学, 教授
PIDDLESDEN サラ  ウェールズ医科大学, 医学部, 研究員
MORGAN B. P  ウェールズ医科大学, 医学部, 教授
松尾 清一  名古屋大学, 医学部, 助手 (70190410)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥7,900,000 (Direct Cost: ¥7,900,000)
Fiscal Year 1997: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1996: ¥3,900,000 (Direct Cost: ¥3,900,000)
KeywordsComplement / Animals / DAF / MCP / CD59 / 512Ag / Crry / C5a receptor / モルモット / ラット / C5_aレセプター / オカダ酸 / 補体制御膜因子
Research Abstract

Species specitic membrane inhibitors of complement such as DAF,MCP and HRF20 (CD59) were identified in experimental animals. We have previously purified guinea pig DAF and rat 512 Ag and cloned cDNA of the molecules. Since guinea pig DAF was found to have several isotypes due to alternative splicing of mRNA,cDNA of each isotypes were transfectedinto CHO cells and the function of those geneproducts on the transfected cells were analyzed. We found that the longer the S/T rich region, the stronger the inhibitory function was observed.
We have also cloned cDNa of guinea pib MCP,mouse DAF,mouse MCP,mouse CD59, rat MCP,and rat DAF.Mouse DAFcDNA was transfectedinto CHO cells and monoclonal antibodies to mouse DAF were generated by immunizing the transfected CHO cells into hamuster and making hybridoma cells fusing the spleen cells with mouse myeloma cells. Tissue distribution of guinea pig DAF,mouse DAF and rat DAF was determined by immunostaining with monoclonal antibodies. RT-PCR methodand Western blotting methodwere also succfessfully used to determine the tissues expressing guinea pig DAF and MCP,mouse DAF,MCP and CD59 as well as rat DAF,MCP and CD59. Furthermore we have also cloned cDNA of anaphylatoxin receptors of mouse and rat.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Akatsu,H. et al.: "Unipue expression of HRF20(CD59)in human nervous tissue." Microbiol.Immunol.41. 321-330 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akatsu,H. et al.: "cDNA cloning and characterization of rat C5a anaphylatoxin receptor." Microbiol.Immunol.41. 575-580 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akahori,T. et al.: "Role of sialyl Lewisx-like epitope selectively expressed on vascular endothelial cells in lokal skin inflammation of the rat." J.Immunol.158. 5384-5392 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawamoto,Y. et al.: "The serum resistance of malaria-infected erythrocytes." Immunology,. 91. 7-12 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tada,T. et al.: "Membrane attack complex of complement and 20kDa homologous resriction facter(CD59)in myocardial infarction." Virchows Arch.430. 327-332 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naito,A. et al.: "Stability of C3 convertase in the rat classical complement pathway." Microbiol.Immunol.412. 621-624 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nonaka,M. et al.: "A new repetitive sequence uniquely present in the decay-accelerating factor genes." Immunogenetics.47. 246-255 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Farkas,I. et al.: "Neuromal C5a receptor and an associated apoptotic signal transduction pathway." J.Physiol.507. 679-687 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wang,G. et al.: "Functional defferences among multiple isoforms of guinea pig decay accelerating facter(DAF)." J.Immunol.(in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akatsu, H., et.al.: "Unique expression of HRF20 (CD59) in human nervous tissue." Microbiol.Immunol.41. 321-330 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akatsu, H., et.al.: "cDNA cloning and characterizationb of rat C5a anaphylatoxin receptor." Microbiol.Immunol.41. 575-580 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akahori, T., et.al.: "Role of sialyl Lewisx-like epitope selectively expressed on vascular endothelial cells in local skin inflammation of the rat." J.Immunol.158. 5384-5392 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kawamoto, Y., et.al.: "The serum resistance of malaria-infected erythrocytes." Immunology. 91. 7-12 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tada, T., et.al.: "Membrane attack complex of complement and 20kDa homologous restriction factor (CD59) in myocardial infarction" Virchow Arch.430. 327-332 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Naito, A.et.al.: "Stability of C3 convertase in the rat classical complement pathway." Microbiol.Immunol.412. 621-624 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Nonaka, M., et.al.: "A new repetitive sequenceuniquely present in the decay-accelerating factor genes." Immunogenetics. 47. 264-255 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Farkas, I., et al.: "Neuronal C5a receptor and associatedapoptotic signal transduction pathway." J.Physiol.507. 679-687 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Wang, G., et.al.: "Functional differences among multiple isoforms of guinea pig decay accelerating factor (DAF)." J.Immunol.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akatsu, H.et al.: "Unipue expression of HRF20 (CD59) in human nervous tissue." Microbiol.Immunol.41. 321-330 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akatsu, H.et al.: "cDNA cloning and characterization of rat C5a anaphylatoxin receptor." Microbiol.Immunol.41. 575-580 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Akahori, T.et al.: "Role of sialyl Lewisx-like epitope selectively expressed on vascular endothelial cells in lokal skin inflammation of the rat." J.Immunol.158. 5384-5392 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kawamoto, Y.et al.: "The serum resistance of malaria-infected erythrocytes." Immunology,. 91. 7-12 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tada, T.et al.: "Membrane attack complex of complement and 20kDa homologous restriction factor (CD59) in myocardial infarction." Virchows Arch.430. 327-332 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Naito, A.et al.: "Stability of C3 convertase in the rat classical complement pathway." Microbiol.Immunol.412. 621-624 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nonaka, M.et al.: "A new repetitive sequece uniquely present in the decay-accelerating factor genes." Immunogenetics.47. 246-255 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Farkas, I.et al.: "Neuromal C5a receptor and an associated apoptotic signal transduction pathway." J.Physiol.507. 679-687 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Wang, G.et al.: "Functional defferences among multiple isoforms of guinea pig decay accelerating factor (DAF)." J.Immunol.(in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Koike, C., Okada, H. et al.: "Induction of α(1,2)-fucosyltransferase and its effect on α-Gal epitopes in transgenic pig." Xenotransplantation. 3. 81-86 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Wolf-Levin, Okada., H. et al.: "Human pregnancy serum suppress the proliferative response of lymphocytes to autologous PHA-activated T lymphoblasts." Am. J. Reprod. Immunol.35. 63-69 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nishikawa, K., Matsuo, S., Okada, N., Morgan, B.P. and Okada, H.: "Local linflammation caused by a monoclonal antibody which blocks the function of the rat membrane inhibitor of C3 convertase." J. Immunol.156. 1182-1188 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fukuoka, Y., Okada, N. and Okada, H.: "Molecular cloning of murine decay accelerating factor(DAF)by immunoscreening." Int. Immunol.8. 379-385 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Takahashi, M., Okada, H. et al.: "Insulin receptor mRNA in the substantia nigra in Parkinson‘s disease." Neurosci. Letters,. 204. 201-204 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayashi, S., Okada, H., Okada, N., et al.: "Protection of guinea pig cells from rat complement-mediated lysis by the transfection of rat complement regulatory factor 5I2 gene." Xenotransplanmtation,. 3. 217-221 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayashi, S., Okada, H. et al.: "Inhibitory effect of double transfection of xenoendothelial cells using both decay accelerating factor and homologous restriction factor 20 genes on complement dependent cytolysis." J. Surgical Res.61. 165-169 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakamura, M., Okada, H., Okada, N., et al.: "Quantification of the CD55 and CD59, membrane inhibitors of complement on HIV-1 particles as a function of complement-mediated virolysis." Microbiol Immunol.40. 561-567 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Iwata, M., Okada, N and Okada, H. et al.: "Complement plays an essential role in shock following interstinal ischaemia in rats." Clin. Exp. Immunol.106. 156-159 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Baranyi, L., Campbell, W. and Okada, H.: "Antisense homology boxes in C5a receptor and C5a anaphylatoxin. A new method for identification of potentially active peptides." J. Immunol.157. 4591-4601 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hosokawa, M., Nonaka, M., Okada, N., Nonaka, M. and Okada, H.: "Molecular cloning of guinea pig membrane cofactor protein(MCP): Preferential expression in testis." J. Immunol.157. 4946-4952 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koike, C., Okada, H., et al.: "Establishment of a human DAF/HRF20 double transgemic mouse line is not sufficient to suppress hyperacute rejection." Surg. Today, Jpn J Surg.26. 993-998 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Koike, C., Okada, H., et al.: "Establishment of a human DAF/HRF20 double transgemic mouse line is not sufficient to suppress hyperacute rejection." Surg. Today, Jpn J Surg.26. 993-998 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Okada, N., Wu, X. And Okada, H.: "Presence of IgM antibodies which sensitize HIV-1 infected cells to cytolysis by homologous complement in long term survivors of HIV-infection." Microbiol. Immunol.41(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akatsu, H., Okada, N., Okada, H. et al.: "Unique expression of HRF20(CD59)in human nervous tissue." Microbiol. Immunol.41(in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2021-04-07  

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