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Molecular studies on liver cancer dovelopment in humans

Research Project

Project/Area Number 08044332
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionSaitama Cancer Center

Principal Investigator

FUJIKI Hirota  Saitama Cancer Center, Res.Inst., Director, 研究所, 所長兼部長 (60124426)

Co-Investigator(Kenkyū-buntansha) IN Kyoung Li  Ajou University School of Medicine, 所長
HAYASHI Shinichi  Researcher, Saitama Cancer Center, Res.Inst., 研究所・生化学部, 主任研究員 (60144862)
SUEOKA Eisaburo  Researcher, Saitama Cancer Center, Res.Inst., 研究所・血清ウイルス部, 研究員 (00270603)
SUGANUMA Masami  Researcher, Saitama Cancer Center, Res.Inst., 研究所・血清ウイルス部, 主任研究員 (20196695)
LIM In Kyoung  Professor, Ajou University, School of Medicine
Project Period (FY) 1996
Project Status Completed (Fiscal Year 1996)
Budget Amount *help
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsHepato-carcinogenesis / Nodularin / Liver tumor promoter / Androgen receptor / Estrogen receptor / Early response gene / TNF-alpha / Endogenous tumor promoter / Gene expression
Research Abstract

Molecular studies on liver cancer development in humans
1. Expression of TNF-alpha gene and early response genes in primary cultured rat hepatocytes Nodularin, a potent liver tumor promoter strongly induces TNF-alpha mRNA and mRNAs of jun and fos gene families in rat liver two-stage carcinogenesis experiment. We studied whether rat hepatocytes induce expression of TNF-alpha gene and early response genes. Treatment of primary cultured rat hepatocytes with nodularin significantly induced enhancement TNF-alpha gene and stabilization of TNF-alpha mRNA.The effects were similar on the expression of early response genes.
2. Reduction of TGF-beta receptor expression in liver tumor promotion
TGF-beta receptor type 1 and 2 (TGF-beta RI and RII) was diminished in preneoplastic nodules induced by nodularin, indicating that reduced response to TGF-beta in preneoplastic cells in rat liver might be involved in hepatocarcinogenesis by nodularin.
3. Expression of TIS 21 gene in mouse skin two-stage carcino … More genesis experiments
TIS 21 gene is one of the genes induced in Swiss 3T3 fibroblasts by treatment with TPA.TIS 21 gene is thought to be one possible tumor suppressor gene. TIS 21 gene was expressed in normal mouse skin. An initiator, dimethylbenz (a) anthracene (DMBA) alone suppressed the expression, and a tumor promoter, okadaic acid, induced TIS 21 expression. However, the level of the expression was not much changed in papilloma and carcinoma of mouse skin.
To achieve successful collaborations with Korean scientists, we organized the Second Scientific Meeting on mechanisms of carcinogenesis and cancer chemoprevention in January this year in Korea, where 7 Japanese scientists and 9 Korean scientists presented new results. As a result of fruitful discussions at this meeting, a new collaboration on the study of TIS21, a putative tumor suppressor gene, has been started, between Professor Yamamoto's laboratory at the Institute of Medical Science, Tokyo University and Dr.Lim's laboratory at Ajou University. Less

Report

(2 results)
  • 1996 Annual Research Report   Final Research Report Summary
  • Research Products

    (47 results)

All Other

All Publications (47 results)

  • [Publications] Verma,A.K. Fujiki,H.et al.: "Superinduction of mouse epidermal ornithine decarboxylase activity by repeated 12-O-tetradecanoylphorbol-13-acetate" Mol.Cell.Biochem.155. 139-151 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki,H.et al.: "Naturally-derived tumor promoters and inhibitors of carcinogenesis" J.Toxicol-Toxin Reviews. 15(2). 129-156 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suganuma,M.Fujiki,H.et al.: "A new process of cancer prevention mediated through inhibition of tumor necrosis factor α expression" Cancer Res.56. 3711-3715 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komori,A.Fujiki,H.et al.: "Anti-tumor promoting activity of canventol and its synthetic analogs through inhibition of protein isoprenylation" Jpn.J.Cancer Res.87. 875-881 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sueoka,N.Fujiki,H.et al.: "Anti-cancer effects of morphine through inhibition of tumour necrosis factor-α release and mRNA expression" Carcinogenesis. 17(11). 2337-2341 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki,H.et al.: "Japanese green tea as a cancer preventive in humans" Nutrition Reviews. 54.(11). S67-S70 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taylor,C,Fujiki,H.et al.: "Inhibition of protein phosphatase 2A by cyclic peptides modelled on the microcystin ring" BioMed.Chem.Lett.6. 2113-2116 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Quinn,R.J.Fujiki,H.et al.: "Inhibition of protein phosphatase 2A by cantharidin analogues" BioMed.Chem.Lett.6(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki,H.et al.: "Chemoprevention of cancer" Comprehensive Toxicology. (in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sueoka,E.Fujiki,H.et al.: "Expression of TNF-α gene and early response genes by nodularin a liver tumor promoter,in primary cultured rat hepatocytes" J.Cancer Res.Clin.Oncol.(in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hayashi,S-I.et al.: "Identification oa allelic variants of the human CYP1A1 gene" Methods in Enzymology. 272. 226-232 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hayashi,S-I.et al.: "Assay of Vitamin D derivatives and purification of their hydroxylases" Methods in Enzymology. 272(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki,H.et al.: "Toxic Microcystis" CRC Press, 203-232 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sueoka,E.Fujiki,H.et al.: "Harmful and Toxic Algal Blooms" Proceedings of the 7th International Conference on Toxic Phytoplnkton, 483-486 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suganuma,M.Fujiki,H.et al.: "Mechanisms of (-)-epigallocatechin gallate (EGCG) and green tea in inhibition of carcinogenesis" International Conference on Food Factors : Chemistry and Cancer Prevention (in press), (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Verma, A.K., Fujiki, H.et al.: "Superinduction of mouse epidermal ornithine decarboxylase activity by repeated 12-o-tetradecanoylphorbol-13-acetate treatments" Mol.Cell.Biochem.155. 139-151 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki, H.et al.: "Naturally-derived tumor promoters and inhibitors of carcinogenesis" J.Toxicol-Toxin Reviews. 15 (2). 129-156 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suganuma, M., Fijiki, H.et al.: "A new process of cancer prevention mediated through inhibition of tumor necrosis factor alpha expression" Cancer Res.56. 3711-3715 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Komori, A., Fujiki, H.et al.: "Anti-tumor promoting activity of canventol and its synthetic analogs through inhibition of protein isoprenylation" Jpan.J.Cancer Res.87. 875-881 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sueoka, N.Fujiki, H.et al.: "Anti-cancer effects of morphine through inhibition of tumour necrosis factor-alpha release and mRNA expression" Carcinogenesis. 17 (11). 2337-2341 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki, H.et al.: "Japanese green tea as a cancer preventive in humans" Nutrition Reviews. 54 (11). S67-S70 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Taylor, C., Fujiki, H.et al.: "Inhibition of protein phosphatase 2A by cyclic peptides modelled on the microcystin ring" BioMed.Chem.Lett.6. 2113-2116 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Quinn, R.J., Fujiki, H.et al.: "Inhibition of protein phosphatase 2A by cantharidin analogues" BioMed.Chem.Lett.6 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki, H.et al.: "Chemoprevention of cancer" Comprehensive Toxicology. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sueoka, E., Fujiki, H.et al.: "Expression of TNF-alpha gene and early response genes by nodularin, a liver tumor promoter, in primary cultured rat hepatocytes" Journal of Cancer Research and clinical Oncology. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hayashi, S-I.et al.: "Identification oa allelic variants of the human CYP1A1 gene" Methods in Enzymology. 272. 226-232 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hayashi, S-I.et al.: "Assay of Vitamin D derivatives and purification of their hydroxylases" Methods in Enzymology. 272 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Hayashi, S-I.et al.: "Two promoters in expression of estrogen receptor messenger RNA in human breast cancer" Carcinogenesis. 18 (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Fujiki, R.et al.: CRC Press. Toxic Microcystis, 203-232 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Sueoka, E., Fujiki, H.et al.: Proceedings of the 7th International Conference on Toxic Phytoplankton. Harmful and Toxic Algal Blooms, 483-486 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Suganuma, M., Fujiki, H.et al.: (in press). Mechanisms of (-)-epigallocatechin gallate (EGCG) and green tea in inhibition of carcinogenesis, (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1996 Final Research Report Summary
  • [Publications] Verma,A.K.Fujiki,H.et al.: "Superinduction of mouse epidermal ornithine decarboxylase activity by repeated 12-o-tetradecanoylphorbol-13-acetate treatments" Mol.Cell.Biochem.155. 139-151 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujiki,H.et al.: "Naturally-derived tumor promoters and inhibitors of carcinogenesis" J.Toxicol-Toxin Reviews. 15(2). 129-156 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suganuma,M.Fujiki,H.et al.: "A new process of cancer prevention mediated through inhibition of tumor necrosis factor α expression" Cancer Res.56. 3711-3715 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Komori,A.Fujiki,H.et al.: "Anti-tumor promoting activity of canventol and its synthetic analogs through inhibition of protein isoprenylation" Jpn.J.Cancer Res.87. 875-881 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sueoka,N.Fujiki,H.et al.: "Anti-cancer effects of morphine through inhibition of tumour necrosis factor-α release and mRNA expression" Carcinogenesis. 17(11). 2337-2341 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujiki,H.et al.: "Japanese green tea as a cancer preventive in humans" Nutrition Reviews. 54(11). S67-S70 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Taylor,C.Fujiki,H.et al.: "Inhibition of protein phosphatase 2A by cyclic peptides modelled on the microcystin ring" BioMed.Chem.Lett.6. 2113-2116 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Quinn,R.J.Fujiki,H.et al.: "Inhibition of protein phosphatase 2A by cantharidin analogues" BioMed.Chem.Lett.6(in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujiki,H.et al.: "Chemoprevention of cancer" Comprehensive Toxicology. (in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sueoka,E.Fujiki,H.et al.: "Expression of TNF-α gene and early response genes by nodularin,a liver tumor promoter,in primary cultured rat hepatocytes" Journal of Cancer Research and Clinical Oncology. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayashi,S-I.et al.: "Identification oa allelic variants of the human CYP1A1 gene" Methods in Enzymology. 272. 226-232 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayashi,S-I.et al.: "Assay of Vitamin D derivatives and purification of their hydroxylases" Methods in Enzymology. 272(in press). (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hayashi,S-I.et al.: "Two promoters in expression of estrogen receptor messenger RNA in human breast cancer" Carcinogenesis. 18(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Fujiki,H.et al.: "Toxic Microcystis" CRC Press, 203-232 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sueoka,E.Fujiki,H.et al.: "Harmful and Toxic Algal Blooms" Proceedings of the 7th International Conference on Toxic Phytoplankton, 483-486 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Suganuma,M.Fujiki,H.et al.: "Mechanisms of (-)-epigallocatechin gallate (EGCG) and green tea in inhibition of carcinogenesis" International Conference on Food Factors : Chemistry and Cancer Prevention (in press), (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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