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Pressure and Temperature Effects on Protein Solutions

Research Project

Project/Area Number 08045025
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research Field Physical chemistry
Research InstitutionRitsumeikan University

Principal Investigator

SAWAMURA Seiji  Ritsumeikan Univ., Chem., Asso.Prof., 理工学部, 助教授 (10167439)

Co-Investigator(Kenkyū-buntansha) ペィティ グレンフェル  ブリティッシュコロンビア大学, 理学部, 教授
マクギリブレイ ロス  ブリティッシュコロンビア大学, 理学部, 教授
マウク グラント  ブリティッシュコロンビア大学, 医学部, 教授
KATO Minoru  Ritsumeikan univ., Chem., Instructs, 理工学部, 講師 (00241258)
TANIGUCHI Yoshihiro  Ritsumeikan univ., Chem., Prof., 理工学部, 教授 (70066702)
MAUK Grant  Univ.of British Columbia, Biochem.Prof.
MACGILLIVRAY Ross J.A.  Univ.of British Columbia, Biochem.Prof.
PATEY Grenfell N.  Univ.of British Colimbia, Chem., Prof.
MAUK Grant  ブリティッシュコロンビア大学, 医学部, Professor
KOGA Yoshika  ブリティッシュコロンビア大学, 理学部, Senior Ins
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordsprotein / pressure / Lysozyme
Research Abstract

Excess partial molar enthalpy of lysozyme in aqueous alcohol was measured using isothermal titration calorimetry. It was suggested that occupancy by the alcohol of a binding site on the surface of the protein may not be necessary for the alcohol to affect the properties of the protein. Pressure dependences of the solubiity for lysozyme and glycine in water measured. The formaer increased and the latter decreased with increasing pressure though both solubilities increased with increasing temperature. It suggests that the pressrue is one of key parameter to consider the protein hydration in the stand point of the constituent amino acids.
High-pressure FT-IR of Myoglobin (Mb) was measured in the range -10-70゚C.Pressure dependence of the second structure of Mb follows two state model. The structure irreversibly changes to random coil forming intermolecular beta-sheet with increasing temperature though it becomes reversible at low temperature.
It was, succeeded in expressing yeast cytochrome c in E.coli through construction of a plasmid for the co-expression of cytochrome c heme lyase and cytochrome c. The yield of wild-type cytochrome c from this system is relatively high. Variants in which the crucial Met80 ligand to the heme iron is replaced with Ala and that do no support respiration can be expressed in E.coli demonstrated that Lys72 is not trimethyllated postsynthetically as it is when expressed in yeast. This finding is no surprising because presumably E.coli does not posses the transmethylase enzyme that catalyzes this modification reaction.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (105 results)

All Other

All Publications (105 results)

  • [Publications] T.Nakai: "Effects of Pressure and Temperature on the viscosity β coefficient for Potassium Chloride in Water" Mutter.Sei.Res.Int.,. 3. 204-209 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Takeda: "Pressure-inaucea structural rearrangemerts of bovine pancreatic trypsin inhibitor studied by FTIR spectroscopy" Biospectroscopy. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsumoto: "Effect of Pressure on the mechanism of hyarolysis of maltoteraose,maltopenthose,and maltohexose Catalyzei by porcine pancreatic α-amylase" Biochim,Bicphys,Acta,. 134・3. 243-250 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fumino: "NMR Studies on dynamic structure of hydrated water molecules in tetraalkylammonium bromide-D_2O solutions at 5-25℃" J.Mol.Liquids,. 72 (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fumino: "Concentration dependence of nuclear magnetic relaxation rates of alkalimetal ionic nucliein dilute solutions." Denki Kagaku. 65. 198-203 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Kogh: "“Intermolecular Interactions"in Agueous Solutions of Three Components including Lysozyme" Fluid Phase Equil,. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.J.F.Day: "Ion Solration in Binavy Mixtares" J.chem.Phys.106. 2782-2793 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Ayton: "Liquid Crystal Phases of Dipolar Discotic Particles" Phys.Rer.E. 55. 447-454 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Twitchett: "Intramolecular Electron Transfer Kinetics of a Synthetic Flavocytochrome C" J.Am.Chem.Soc.119. 435-436 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Bujons: "Charge,Reversal of a Critical Actire Site Residue of Cytochrome C Peroxidase:Characterization of the AFS.48 Glu Variant" Eur.J.Biochem. 243. 72-84 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.L.Hunter: "Role of Heme Propionates in the Interaction of Heme with Apomyoglobin and Apocytochrome b_5." Biochemistry. 36. 1010-1017 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.L.Hunter: "Dissociation of Heme from Myoglobin and Cytochrome b_5:Comparison of Behaviorin Solution and the Gas Phase" Biochemistry. 36. 1018-1025 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] P.Westh: "Intermolecular Interactions of Lysozyme and Small Alcohols:A Calorimetric Investigation" J.Phys.Chem.B101. 5755-5758 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Xu: "Light Scattering Study of Sol-Gel Transformation of SiO_2 Colloid at Extremely High Concentrations" Clays and Clay Minerals. (印刷中). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] D.R.Berard: "Structure of the Metal-Aqueous Electrolyte Solution Intertace" J.chem.phys.107. 4719-4728 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.C.Shelley: "Modeling and Structure of Mercury-Water Interfaces" J.chem.Phys.107. 2122-2141 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Ayton: "Ferroelectric and Dipolar Glass Phases of Non-Crystalline Systems" Phys.Rev.E. 56. 562-570 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.M.Cann: "An Investigation of the Influence of Solute Size and Insertion on solvation Thermodynamics" J.Chem.Phys.106. 8165-8195 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.M.Nocek: "Cyclic Electron Transfer within the[Zn-deuteremyoylobin,Cytochrome b_5]Complex:Redox-Linked changes at the Interface" J.Am,chem,Loi.119. 2146-2155 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.G.Mank: "Control of Metalloprotein Redox Potentials:What Does Site-Directed Mutagenesis of Hemoproteins Tell Us?" J.Biol.Inorg.Chem.2. 119-125 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Kishi: "Site-Directed Mutations of Phenylalanire-190 of Manganese Peroxidase:Effects on Stability,Function,and Coordination" Biochemistry. 36. 4268-4277 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] L.Konermann: "Acid-Induced Denaturation of Myoglobin Studied by Time-Resolved Electrospray Ionization Mass Spectrometry" Biochemistry. 36. 6448-6454 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Maurns: "A Myoglobin Variant with a polar Substitution in Conserved Hydrophobic Cluster in the Heme Binding Pocket" Biochin.Biophys,Actu.,. 1341. 1-13 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cote H.C.F: "A novel approach for the characterization and epitope determination of alupus anticoagulant-associated neutralizing anti-prothrombin antibody" Am.J.Clin.Putho.107. 197-205 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cote,H.C.F.: "Functional characterization of recombinant human meizothrombin and meizothrombin(DesF1)" J.Biol.Chem.272. 6194-6200 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.A.Brown: "Vectors for expressing proteins at the amino-terminus of an activation domain for use in the yeast two-hybrid system" Anal.Biochem.247. 451-452 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Q-Y.He: "Effects of mutations of aspartic acid63 on the metal-binding properties of the recombinant N-lobe of human serum transferrin" Biochemistry. 36. 5522-5528 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] L.A.Luck: "19F NMR studies of the recombinant human transferrin N-lobe and three singlepoint mutants" Mag.Reson.Chem,. 35. 477-481 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.B.Mason: "Receptor recognition sites reside in both lobes of humann serun transfenin" Biochem.J.326. 77-85 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] O.Zak: "A kinetically active site in the C-lobe of human tramsferrin" Biochemistry. 36. 11036-11043 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.M.Guarna: "On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia Pastoris" Biotechnol.Bioeng,. 56. 279-286 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Q-Y.He: "Inequivalence of the two tyrosine ligandsin the N-lobe of human serum transferrrin" Biochemistry. 36. 14853-14800 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 谷口 吉弘: "生物・環境産業のための非加熱プロセス事典" サイエンスフォーラム, 95-99 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nakai, S.Sawamura, Y.Taniguchi, and T.Kuboyama: "Effects of Pressure and Temperature on the Viscosity B Coefficient for Potassium Chloride in Water" Matter.Sci.Res.Int.3. 204-209 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Takeda, K.Nakano, M.Kato and Y.Taniguchi: "Pressure-induced Structural Rearrangements of Bovine Pancreatic Trypsin Inhibitor Studies by FTIR Spectroscopy" Biospectroscopy. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Matsumoto, S.Makimoto, and T.Taniguchi: "Effect of Pressure on the Mechanism of Hydrolysis of Maltoteraose, Maltopentaose, and Maltohexose Catalyzed by Porcine Pancreatic alpha-amylase" Biochim.Biophys.Acta. 1343. 243-250 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fumino, K.Yukiyasu, A.Shimizu, and Y.Taniguchi: "NMR Studies on Dynamic Structure of Hydrated Water Molecules in Tetraalkylammonium Bromide-D_2O solutions at 5-25゚C" J.Mol.Liquids. 72 (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Fumino, A.Shimizu, and Y.Taniguchi: "Concentration Dependence of Nuclear Mgnetic Relaxation Rates of Alkali Metal Ionic Nuclie in Dilute Solutions" Denki Kagaku. 65. 198-203 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Koga, P.Westh, Ch.Trandum, and C.A.Haynes: ""Intremolecular Interactions" in Aqueous Solutions of Three Components including Lysozyme" Fluid Phase Equil.(in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Taniguchi: "Property of Water and Aqueous Solution under High Pressure" Dictionary of Non-heating Process for Life-and Circumstances-Industry. 95-99 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.J.F.Day and G.N.Patey: "Ion Solvation in Binary Mixtures" J.Chem.Phys.106. 2782-2793 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Ayton, D.Q.Wei.and G.N.Patey: "liquid Crystal Phases of Dipolar Discotic Particles" Phys.Rev.E.55. 447-454 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Twitchett, J.C.Ferrer, P.Siddarth, and A.G.Mauk: "Intramolecular Electron Transfer Kinetics of a Synthetic Flavocytochrome c" J.Am.Chem.Soc.119. 435-436 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.Bujons, A.Dikiy, J.C.Ferrer, L.Banci, and A.G.Mauk: "Change Reversal of a Critical Active Site Residue of Cytochrome c Peroxidase : Characterization of the Arg48Glu Variant" Eur.J.Biochem.243. 72-84 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.L.Hunter, E.Lloyd, L.D.Eltis, S.P.Rafferty, H.Lee, M.Smith, and A.G.Mauk: "Role of Heme Propionates in the Interaction of Heme with Apomyoglobin and Apocytochrome b" Biochemistry. 36. 1010-1017 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] C.L.Hunter, A.G.Mauk, and D.J.Douglas: "Dissociation of Heme from Myoglobin and Cytochrome b_5 : Comparison of Behavior in Solution and the Gas Phase" Biochemistry. 36. 1018-1025 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] P.Westh and Y.Koga: "Intermolecular Interactions of Lysozyme and Small Alcohols : A Calorimetric Investigation" J.Phys.Chem.B. 101. 5755-5758 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Xu and Y.Koga: "Light Scattering Study of Sol-Gel Transformation of SiO_2 Colloid at Extremely High Concentrations" Clays and Clay Minerals. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] D.R.Berard, M.Kinoshita, N.M.Cann, and G.N.Patey: "Structure of the Metal-Aqueous Elctrolyte Solution Interface" J.Chem.Phys.107. 4719-4728 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.C.Shelley, G.N.Patey, D.R.Berard, and G.M.Torrie: "Modeling and Structure of Mercury-Water Interfaces" J.Chem.Phys.107. 2122-2141 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] G.Ayton, M.J.P.Gingras, and G.N.Patey: "Ferroelectric and Dipolar Glass Phases of Non-Crystalline Systems" Phys.Rev.E. 56. 562-570 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.M.Cann and G.N.Patey: "An Investigation of the Influence of Solute Size and Insertion on Solvation Thermodynamics" J.chem.Phys.106. 8165-8195 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.M.Nocek, B.P.Sishta, J.C.Cameron, A.G.Mauk, and B.M.Hoffman: "Cyclic Electron Transfer within the [Zn-deuteromyoglobin, Cytochrome b_5] Complex : Redox-Linked Changes at the Interface" J.Am.Chem.Soc.119. 2146-2155 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.G.Mauk and G.R.Moore: "Control of Metalloprotein Redox Potentials : What Does Site-Directed Mutagenesis of Hemoproteins Tell Us?" J.Biol.Inorg.Chem.2. 119-125 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Kishi, D.P.Hildebrand, M.Kusters-van Someren, J.Gettemy, A.G.Mauk, and M.H.Gold: "Site-Directed Mutations of Phenylalanin-190 of Manganese Peroxidase : Effects on stability, Function, and Coordination" Biochemistry. 36. 4268-4277 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] L.Konermann, F.I.Rosell, A.G.Mauk, and D.J.Douglas: "Acid-Induced Denaturation of Myoglobin Studies by Time-Resolved Electrospray Ionization Mass Spectrometry" Biochemistry. 36. 6448-6454 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] R.Maurus, C.M.Overall, R.Bogunil, Y.Luo, A.G.Mauk, M.Smith, and G.D.Brayr: "A Myoglobin Variant with a Polar Substitution in a Conserved Hydrophobic Cluster in the Heme Binding Pocket" Biochem.Biophys.Acta. 1341. 1-13 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.C.F.Cote, D.G.Huntsman, J.Wu, L.D.Wadsworth, and R.T.A.MacGillivray: "A Novel Approach for the Characterization and Epitope Determination of a Lupus Anticoagulant-associated Neutralizing Anti-prothrombin Antibody" Am.J.Clin.Pathol.107. 197-205 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.C.F.Cote, L.Bajzar, W.K.Stevens, J.A.Samis, J.Morser, R.T.A.MacGillivray, and M.E.Nesheim: "Functional Characterization of Recombinant Human Meizothrombin and Meizothrombin (DesF1). Thrombomodulin-dependent Activation of Protein C and Thrombin-activatable Fibrinolysis Inhibitor (TAFI), Platelet Aggregation, Antithrombin-III Inhibition" J.Biol.Chem.272. 6194-6200 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.A.Brown and R.T.A.MacGillivray: "Vectors for Expressing Proteins at the Amino-terminus of an Activation Domain for Use in the Yeast Two-hybrid System" Anal.Biochem.247. 451-452 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Q.-Y.He, A.B.Mason, R.C.Woodworth, B.M.Tam, T.Wadsworth, and R.T.A.MacGillivray: "Effects of Mutations of Aspartic Acid 63 on the Metalbinding Properties of the Recombinant N-lobe of Human Serum Transferrin" Biochemistry. 36. 5522-5528 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] L.A.Luck, A.B.Mason, K.J.Savage, R.T.A.MacGillivray, and R.C.Woodworth: "19F NMR Studies of the Recombinant Human Transferrin N-lobe and Three Single Point Mutants" Mag.Reson.Chem.35. 477-481 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.B.Mason, R.C.Woodworth, R.W.A.Oliver, B.N.Green, L.-N.Lin, J.F.Brandts, B.M.Tam, K.J.Savage, J.A.Lineback, and R.T.A.MacGillivray: "Receptor Recognition Sites Reside in Both Lobes of Human Serum Transferrin" Biochem.J.326. 77-85 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] O.Zak, B.Tam, R.T.A.MacGillivray, and P.Aisen: "A Kinetically Active Site in the C-lobe of Human Transferrin" Biochemistry. 36. 11036-11043 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.M.Guama, G.L.Lesnicki, B.Tam.J.Robinson, C.Z.Radziminski, D.Haseniwinkle, A.Boraston, E.Jervis, R.T.A.MacGillivray, R.F.B.Turner, and D.G.Kilburn: "On-line Monitoring and Control of Methanol Concentration in Shake-flask Cultures of Pichia pastoris" Biotechnol.Bioeng.56. 279-286 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Q.-Y.He, A.B.Mason, R.C.Woodworth, B.M.Tam, R.T.A.MacGillivray, J.K.Grady, and N.D.Chasteen: "Inequivalence of the Two Tyrosine Ligands in the N-lobe of Human Serum transferrin" Biochemistry. 36. 14853-14860 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T.Nakai: "Effects of Pressure and Temperatur on the viscosity 13 coefficiant for Potassium Chloride in Water" Matter.Sci.Res.Int.,. 3. 204-209 (1997)

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      1997 Annual Research Report
  • [Publications] N.Takeda: "Pressure-induced Structural rearrangements of bovine pancreatic trypsin inhibitor studied by FTIR Spectroscopy" Biospectroscopy. 印刷中. (1998)

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      1997 Annual Research Report
  • [Publications] T.Matsumoto: "Effect of Pressure on the mechanism of hydrolysis of malcternose,maltopcrvtaose,and maltohexose catalyzed by porcine paticieatic α-amylase" Biochim.Biophys.Acta.1343. 243-250 (1997)

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  • [Publications] K.Fumino: "NMR Studies on dynamic structure of hydrated water molecules in tetraalkylammonium bromide-D_2O Solutions at5-25℃" J.Mol.Liquids,. 72、(印刷中).

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      1997 Annual Research Report
  • [Publications] K.Fumino: "Concontration dependence of nuclear magnetic relaxation vntes of alkalimetal ionic nucliein dilute solutions" Denki Kagaku. 65. 198-203 (1997)

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  • [Publications] Y.Koga: ""Intermolecular Interactions"in Aqueous Solutions of Three Components including Lysozyme" Fluid Phase Egnil.(印刷中). (1998)

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      1997 Annual Research Report
  • [Publications] I.J.F.Day: "Ion Solration in Binavy Mixtares" J.Chem.Phys.106. 2782-2793 (1997)

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      1997 Annual Research Report
  • [Publications] G.Ayton: "Liquid Crystal Phases of Dipolar Discotic Particles" Phys.Rer.E. 55. 447-454 (1997)

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      1997 Annual Research Report
  • [Publications] M.Twitchett: "Intramolecular Electron Transfer Kinetics of a Synthetin Flavocytochrome C" J.Am.Chem.Soc.119. 435-436 (1997)

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      1997 Annual Research Report
  • [Publications] J.Bujons: "Charge Reversal of a Critical Active Site Residue of Cytochrome C Peroxidase:Cfaracterixation of the Afs 48Glu Variant" Eur.J.Biochem. 243. 72-84 (1997)

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      1997 Annual Research Report
  • [Publications] C.L.Hunter: "Role of Heme Propionates in the Interaction of Heme with Apomyoglobin and Apocytochrome b5." Biochemistry. 36. 1010-1017 (1997)

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      1997 Annual Research Report
  • [Publications] C.L.Hunter: "Dissociation of Heme from Myoglobin and Cytochrome b5:Comparison of Behavior in Solution and the Gas Phase" Biochemistry. 36. 1018-1025 (1997)

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      1997 Annual Research Report
  • [Publications] J.M.Nocek: "Cyclic Electron Transfer with in the [Zn-Deuteremyoglobin,Cytochromb5]Complex:Redox-Linked Changes at theInterface" J.Am.Chem.Lec.119. 2146-2155 (1997)

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  • [Publications] A.G.Mank: "Control of Metalloprotein Redox Potentials:What Does Site-Directed Mutagenesis of Hemoproteins Tell Us ?" J.Biol.Inors.Chem.2. 119-125 (1997)

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      1997 Annual Research Report
  • [Publications] K.Kishi: "Site-Directed Mutations of Phonylalanire-190 of Manganese Peroxidase:Effects on Stability,Function and Coordination" Biochemistry. 36. 4268-4277 (1997)

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  • [Publications] L.Konermann: "Acid-Induced Donaturation of Myoglobin Studied by Time-Resolved Electrospray Ionization Mass Spectrometry" Biochemistry. 36. 6448-6454 (1997)

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  • [Publications] R.Maurus: "A Myoglobin Variant witha Polar Substitution in Conserved Hydrophobic Cluster in the Heme Binding Pocket" Biochim.Biophys.Acta.1341. 1-13 (1997)

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  • [Publications] Cote.H.C.F: "A Novel approach for the characterization and epitope determination of alupus anticoagnlant-associatedneutralizing anti-prothrombin antibody" Am.J.Clin.Phthol.107. 197-205 (1997)

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  • [Publications] P.Westh: "Intermolecular Interactions of Lysozyme and Small Alcohols:A Calorimetric Investigation" J.Phys.Chem. B101. 5755-5758 (1997)

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  • [Publications] Y.Xu: "Light Scattering Study of Sol-Gel Trsnsformation of Sio_<> Colloid at Extremely High Concentrations" Clays and Clay Minerals. (印刷中). (1998)

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  • [Publications] D.R.Berard: "Structive of the Metal-Aqueous Electrolyte Solution Interface" J.Chem.Phys.107. 4719-4728 (1997)

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  • [Publications] J.C.Shelley: "Modeling and Structure of Mercury-Water Interface" J.Chem.Phys.107. 2122-2141 (1997)

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  • [Publications] G.Ayton: "Ferroelectic and Dipolar Glass Phases of Non-Crystalline Systems" Phys.Rev.E. 56. 562-570 (1997)

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  • [Publications] N.M.Cann: "An Investigation of the Infulence of Solute Size and Insertion on Solvation Thermodynamics" J.Chem.Phys.106. 8165-8195 (1997)

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  • [Publications] Cote.H.C.F.: "Functional Characterization of recombinant human meizo-thrombin and meizothrombin(DesF1)" J.Biol.Chem.272. 6194-6200 (1997)

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  • [Publications] M.A.Brown: "Vectors for expressing proteinsat the amino-terminus of an activationdomain for use in the yeast two-hybridsystem" Anal.Biochem.247. 451-452 (1997)

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  • [Publications] Q.Y.He: "Effects of mutations of aspartic acid63 on the metal-binding properties of the vecanbinant N-lobe of humanserum transferrin" Biochemistry. 36. 5522-5528 (1997)

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  • [Publications] L.A.Luck: "19F NMR Studies of the recombinant human transferrin N-lobe and three singlepoint mutants" Mag.Reson.Chem.35. 477-481 (1997)

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  • [Publications] A.B.Mason: "Receptor recognition Sites reside in both lobes of Humann Serum transferrin" Biochem.J.326. 77-85 (1997)

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      1997 Annual Research Report
  • [Publications] O.Zak: "A kinetically active site in the C-lobe of human transferrin" Biochemistry. 36. 11036-11043 (1997)

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  • [Publications] M.M.Guarna: "On-line monitoring and control of methanol concentration in Shake-flask cultures of Pichia pastoris" Biotechnol.Bioerg.56. 279-286 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Q-Y.He: "Inequivalence of the two tyrosine ligandsin the N-lobe of human serumtransferrin" Biochemistry. 36. 14853-14860 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷口 吉弘: "生物・環境産学のための非加熱プロセス事典" サイエンスフォーラム, 95-99 (1997)

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  • [Publications] N.Takeda,M.Kato,Y.Taniguchi: "Pressure-and Thermally-Induced Reversible Changes in the Secondary Structure of Ribonuclease A Studied by FT-IR Spectroscopy" Biochemistry. 34. 5980-5987 (1996)

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  • [Publications] K.Koga,P.Westh,S.Sawamura and Y.Tanigucgi: "Normalized Fluctuations,H_2O vsn-Hexane Site-Correlated Percolation" J.Chem.Phys.105. 2028-2033 (1996)

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  • [Publications] Y.Koga: "Mixing Schemes in Agaeons Solutions of Non electrolytes : A Thermodynamic Approach" J.Phys.Chem.100. 5172-5181 (1996)

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      1996 Annual Research Report
  • [Publications] E.Lloyd,D.L.Burk,J.C.Ferrer,R.Maurus,J.Doran,P.R.Carey,G.D.Brayer,A.G.Mauk: "Electrostatic Modification of the Active Site of Myoglobin : Characterization of the Proxinal Ser92 ASP Variant" Biochemistry. 35. 11901-11912 (1996)

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  • [Publications] S.P.Rafferty,J.G.Guillemette,A.M.Berghuis,M.Smith.G.D.Brayer,A.G.Mauk: "Mechanistic and Stractural Contributions of Gitical Surface and Internal Residues to Cytochrone C Electron Transfer Reactivity" J.Biomem.Bioenerg.(発表予定).

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  • [Publications] P.Westh,Y.Koga: "Intermolecular Interactions of Lysozyme and Small Alcohols : A Calorimetric Investisation" J.Phys.Chem.(発表予定).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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