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Induction Mechanisms of Cholangiocarcinoma by Liver Fluke

Research Project

Project/Area Number 08045064
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research Field Human pathology
Research InstitutionMie University

Principal Investigator

ANDO Katsuhiko (1998)  Mie Univ., Faculty of Medicine, Asso.Professor, 医学部, 助教授 (90024710)

矢谷 隆一 (1996-1997)  三重大学, 医学部, 教授 (80024636)

Co-Investigator(Kenkyū-buntansha) VANCHAI Vata  タイ, コンケーン大学・医学部, 教授
CHINZEI Yasuo  Mie Univ., Faculty of Medicine, Professor, 医学部, 教授 (60024709)
KAWARADA Yoshifumi  Mie Univ., Faculty of Medicine, Professor, 医学部, 教授 (40024814)
SAKAKURA Teruyo  Mie Univ., Faculty of Medicine, Professor, 医学部, 教授 (80073120)
YATANI Ryuichi  Mie Univ.President, 学長 (80024636)
VATANASAPT Vanchai  Khon Kaen Univ., Faculty of Medicine, Professor
安藤 勝彦  三重大学, 医学部, 助教授 (90024710)
TESANA Smarn  タイ, コンケーン大学・医学部, 助教授
PAIROJKUL Ch  タイ, コンケーン大学・医学部, 教授
SITHITHAWORN パイブーン  タイ, コンケーン大学・医学部, 助教授
VATANASAPT V  タイ, コンケーン大学・医学部附属病院, 教授
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1996: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsOpisthorchis viverrini / cholangiocarcinoma / epidemiology / animal experiment
Research Abstract

Infection rate of Opisthorchis viverrini (Ov) in snail and fish were surveyed, at 2 lakes located in Northeast Thailand. No Ov cercariae were detected in snails. Whereas Ov metacercariae were detected in cyprinoid fish caught at both lakes.
These metacercariae were given to hamster to induce cholangiocell carcinomas.
Chemical carcinogen (DMN) induced cholangiocell carcinomas in 30% of the animals at 23 weeks after the termination of the treatment. An infection with Ov promoted the carcinogenesis to 100%. The preimmunization of the animals by crude extracts of Ov influenced the cancer development variously, regulating and progressing. Ov infection alone resulted in a variety of pathological changes such as dilatation of bile duct, bile ductule prolifertation, and liver cirrhosis but not cancer. Oncofetal type of extracellular matrix tenascin was examined by immunohistochemistry. The positive staining was found in connective tissues surrounding the dilatated ducts and the stroma of cancers, and strongly in the stroma flame of necrotic cancer nodules.
Nineteen Thai cases of cholangiocellular carcinoma (CCC) and 23 Japanese cases of CCC were compared for clinicopathological and biological features. A review of histological type revealed poorly differentiated and mucinous adenocarcinoma to be somewhat more prevalent in the Thai cases, and peribiliary fibrosis and adenomatous hyperplasia were found to be much more frequent in the Thai cases in noncancerous area of the liver. No significant differences in expression of p53 protein or c-erbB.2 protein were found between the two series, but proliferative activity evaluated by mean MIB1 L.I.was significantly higher in the Thai cases.
Nucleotide sequence of ITS2 in Ov ribosomal DNA was determined. They were compared among Ov taken from three districts (Chonnabot, Chaiyaphum and Kalasin) in northeast Thailand but there was no difference among them.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] 鎮西康雄: "タイ肝吸虫と胆管癌" 病理と臨床. 15. 189 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 安藤勝彦: "希望と苦悩のアジア(分担執筆)" 三重大学出版会, 261 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yasuo Chinzei: "Opisthorchis viverrini and Cholangiocarcinoma" Byouri to Rinsho. Vol.15. 189 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Katsuhiko Ando: Kibou to kunou no Ajia.Mie Acadeic Publishing, 261 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 鎮西康雄: "タイ肝吸虫と胆管癌" 病理と臨床. 15. 189 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 安藤勝彦: "希望と苦悩のアジア(分担執筆)" 三重学術出版会, 261 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 鎮西 康雄: "タイ肝吸虫と胆管癌" 病理と臨床. 15. 189 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 安藤 勝彦: "希望と苦悩のアジア(分担執筆)" 三重学術出版会, 261 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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