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Molecular Science on the Specific Roles of Metal Ions in Biological Functions.

Research Project

Project/Area Number 08249105
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionOkazaki National Research Institutes

Principal Investigator

KITAGAWA Teizo  Okazaki National research Institutes Professor, 分子科学研究所, 教授 (40029955)

Co-Investigator(Kenkyū-buntansha) KIMURA Eiichi  Okazaki National Research Institutes, 医学部, 教授 (30034010)
NISHINO Takeshi  Nihonn Medical College Professor, 教授 (40094312)
MORISHIMA Isao  Kyoto University Professor, 工学部, 教授 (50026093)
井口 洋夫  岡崎国立共同研究機構, 分子科学研究所, 名誉教授 (00100826)
渡辺 芳人  岡崎国立共同研究機構, 分子科学研究所, 教授 (10201245)
伊藤 光男  岡崎国立共同研究機構, 分子科学研究所, 所長 (20013469)
Project Period (FY) 1996 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥43,100,000 (Direct Cost: ¥43,100,000)
Fiscal Year 2000: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥11,600,000 (Direct Cost: ¥11,600,000)
Fiscal Year 1998: ¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1997: ¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1996: ¥14,200,000 (Direct Cost: ¥14,200,000)
KeywordsMetallo-proteins / Metallo-nuclic acid / biometals / gene control / heme proteins / cytochromes / cytochrome oxidase / oxygen activation / 生体分子科学 / 構造生物学 / 分子生物学 / 金属タンパク質 / 金属核酸 / 人工変異タンパク質 / 分子認識 / 生体金属 / 生体エネルギー / 生体情報伝達 / 小分子活性化 / 生体無機化学
Research Abstract

The project members consisted of four teams focused on investigations of energy conversion, material transformation, signal transduction, and gene expression. Members were encouraged to participate in various kinds of collaborative networks of researchers having different expertise in physical chemistry, coordination chemistry, biochemistry, and molecular biology. Eleven major topics were extensively studied by the four teams including energy conversion in photosynthesis and respiration, activation mechanisms of small molecules by biometals, signal initiation, transduction and reception by metalloproteins, and regulation of gene expression by biometallic compounds. Each of the four teams consisted of the several core members, who were selected by the committee of leaders of the four teams, and the recruited members, who were selected from general applicants by a committee of the Ministry of Education, Science, Sports, and Culture. The total numbers of members were 67, 57, 58, and 50 for the 1^<st>, 2^<nd>, 3^<rd>, and 4^<th> years. The number and composition of members were changed every year. All members were requested to present new results as posters in a two-day open workshop once a year, to which a few distinguished foreign bioinorganic chemists were invited to evaluate the results and to deliver a lecture. The activity has been summarized as SBIC News in J.Biol.Inorg.Chem. 5, 410-415 (2000). The rich accomplishments of individual members are published as focused reviews and original papers in a special issue of J.Inorg.Biochem. 82, #1-4 (2000).

Report

(6 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] T.Tomita et al.: "Resonance Raman investigation of Fe-N-O structure of nitrosylheme in myoglobin and its mutants."Journal of Physical Chemistry Part B. 103. 7044-7054 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Iwase et. al.: "Infrared evidence for Cu_<B-> ligation of photodissociated CO of cytochrome c oxidase at ambient temperatures and accompanied deprotonation of a carboxyl side chain of protein."Journal of American Chemical Society. 121. 1415-1416 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Iwasaki et al.: "Modulation of the remote heme site geometry of recombinant mouse neuronal nitric oxide synthase by the N-terminal hook region"Journal of Biological Chemistry. 274. 7705-7713 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] E.Kimura et al.: "Dynamic Enolate Recognition in Aqueous Solution by Zinc (II) in a Phenacyl-Pendant Cycle Complex : Implications for the Role of Zinc (II) in Class II Aldolases."Journal of American Chemical Society. 121. 1267-1274 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Goto et al.: "Mechanisms of Sulfoxidation Catalyzed by High-Valent Intermediates of Heme Enzymes : Electron Transfer vs Oxygen Transfer Mechanism."Journal of American Chemical Society. 121. 9497-9502 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kitagawa: "Progress report on molecular biometallics (1996-2000), a project of the priority areas for research under the auspices of the Japanese Government"Journal Biological Inorganic Chemistry. 5. 410-415 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Kitagawa: "Special Issue on Japanese Molecular Biometallics"Journal Inorganic Biochemistry. 82, issues. 1-4 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Tomita et al.: "Resonance Raman investigation of Fe-N-O structure of nitrosylheme in myoglobin and its mutants."Journal of Physical Chemistry Part B. 103. 7044-7054 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Iwase et.al.: "Infrared evidence for Cu_B ligation of photodissociated CO of cytochrome c oxidase at ambient temperatures and accompanied deprotonation of a carboxyl side chain of protein."Journal of American Chemical Society. 121. 1415-1416 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Iwasaki et al.: "Modulation of the remote heme site geometry of recombinant mouse neuronal nitric oxide synthase by the N-terminal hook region"Journal of Biological Chemistry. 274. 7705-7713 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] E.Kimura et al.: "Dynamic Enolate Recognition in Aqueous Solution by Zinc(II) in a Phenacyl-Pendant Cycle Complex : Implications for the Role of Zinc(II) in Class II Aldolases."Journal of American Chemical Society. 121. 1267-1274 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Goto et al.: "Mechanisms of Sulfoxidation Catalyzed by High-Valent Intermediates of Heme Enzymes : Electron Transfer vs Oxygen Transfer Mechanism."Journal of American Chemical Society. 121. 9497-9502 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] T. Tomita et al.: "Resonance Raman investigation of Fe-N-O structure of nitrosylheme in myoglobin and its mutants"Journal of Physical Chemistry Part B. 103. 7044-7054 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Iwase et al.: "Infrared evidence for Cu_B ligation of photodissoclated CO of cytochrome c oxidase at ambient temperatures and accompanied deprotonation of a carboxyl side chain of protein"Journal of American Chemical Society. 121. 1415-1416 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Shirai et al.: "Crystal Structure of a Protein with an Artificial Exon-Shuffling Module-Substituted Chimera Hemoglobin ba(F133V) at 2.5A Resolution"Journal of Molecular Biology. 287. 369-382 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Murakami et al.: "Effects of the Arrangement of a Distal Catalytic Residue on Regioselectivity and Reactivity in the Coupled Oxidation of Sperm Whale Myoglobin Mutants"Journal of American Chemical Society. 121. 2007-2011 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K. Ichimori et al.: "Inhibition of xanthine oxidase and xanthine dehydrogenase by nitric oxide: nitric oxide converts reduced xanthine oxidizing enzymes into the desulfo-type inactive form"Journal of Biological Chemistry. 274. 7763-7768 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] E. Kimura et al.: "Novel Recognition of Thymine Base in Double-Stranded DNA by Zinc(II)-Macrocyclic Tetraamine Complexes Appended with Aromatic Groups"Journal of American Chemical Society. 121. 5426-5436 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Kitagawa et al.: "Time-resolved resonance Raman investigation of oxygen reduction mechanism of bovine cytochrome c oxidase." J.Bioener.Biomemb.30. 71-79 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Kitagawa et al.: "Time-resolved resonance Raman study of dioxygen reduction by cytochrome c oxidase","Oxygen Homeostasis and its Dynamics"(Y.Ishimura,H.Shimada,and M.Suematsu eds.)" Springer-Verlag.Tokyo. 57-71 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] K.Inaba et al.: "CStructural and functional roles of heme binding module in globin proteins.Identification of the segment regulating the heme binding structure" J.Mol.Biol.282. 311-327 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Nishino et al.: "Conversion of the dehydrogenase type to the oxidase type of rat liver xanthine dehydrogenase by modification of cysteine residues with fluorodinitrobenzene." J.Biol.Chem.272. 29859-29864 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Aoki et al.: "The first selective and efficent transport of imide-containing nucleosides and nucleotides by lipophilic cyclen-zync(II)complexes" Journal of the American Chemical Society. 120. 10018-10026 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] S.Ozaki et al.: "Characterization of polyethylene glycolated horseradish perxidase in organic solvents" Journal of the American Chemical Society. 120. 8020-8025 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Kitagawa, et al.: "Oxygen Activation Mechanism at Binuclear Site of Heme-Copper Oxidase Superfamily Revealed by Time-Resolved Resonance Raman Spectroscopy" Progress in Inorganic Chemistry. 45. 431-479 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] X.Zhao, et al.: "Observation of Resonance Raman Spectra of the Semiquinones,Q_a.and Q_b.in Photosynthetic Reaction Center from Rb.Sphaeroides R26" Journal of the American Chemical Society. 119. 5263-5264 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] M.Tanaka, et al.: "Catalytic Activities and Structure Properties of Horseradish Peroxidase Distal His42 to Glu or Gln Mutant" Biochemistry. 36. 9889-9898 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Hori, et al.: "Calcium Dependent Inactivation of Neuronal Nitric Oxide Synthase : Evidence for the Existence of Stabilization/Activation Factor" Biochemical and Biophysical Research Communication. 234. 476-480 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.Kimura, et al.: "A Tris (Zn^<11>-1,4,7,10-tetraazacyclododecane) Complex as a New Receptor for Phosphate Dianions in Aqueous Solution" Journal of the American Chemical Society. 119. 3068-3076 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Ozaki, et al.: "Conversion of Myoglobin into a Peroxygenase : A Catalytic Intermediate of Sulfoxidation by the F43H/H64L Mutant" Journal of the American Chemical Society. 119. 6666-6667 (1997)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2019-02-15  

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